Cloning, expression and purification of recombinant streptokinase: Partial characterization of the protein expressed in Escherichia coli

Brazilian Journal of Medical and Biological Research

Volumn 30, Issue 12, 1997, Pages 1427-1430

  Avilan L ^a   Yarzabal A ^a,b   Jurgensen C ^a   Bastidas, M ^a   Cruz, J ^a   Puig, J ^a  

^a UNIVERSIDAD DE LOS ANDES   (Venezuela)

^b FACULTAD DE CIENCIAS   (Venezuela)

Author keywords

Plasminogen activators; Protein purification; Recombinant streptokinase

Indexed keywords

ESCHERICHIA COLI; STREPTOCOCCUS; STREPTOCOCCUS DYSGALACTIAE SUBSP. EQUISIMILIS;

RECOMBINANT PROTEIN; STREPTOKINASE;

ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; STREPTOCOCCUS;

CLONING, MOLECULAR; ESCHERICHIA COLI; RECOMBINANT PROTEINS; STREPTOCOCCUS; STREPTOKINASE;

EID: 0031300451     PISSN: 0100879X     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0100-879X1997001200007     Document Type: Article

References (12)

1. Castellino F & Sodetz J (1976). Streptokinase. Methods in Enzymology, 45: 244-257.
2. Tang J & Jackson W (1982). Complete amino acid sequence of streptokinase and its homology with serine proteases. Biochemistry, 21: 6625-6628.
3. Castellino FJ (1979). A unique enzyme-protein substrate modifier reaction: plasmin/streptokinase interactions. Transactions of the International Biochemistry Society, 1: 1-5.
4. Rutherford JD & Braunwald E (1990). Thrombolytic therapy in acute myocardial infarction. Chest, 97: 136-145.
5. Talmadge K, Stahl S & Gilbert W (1980). Eukaryotic signal sequence transports in insulin antigen in Escherichia coli. Proceedings of the National Academy of Sciences, USA, 77: 3369-3373.
6. Malke H & Ferreti J (1984). Streptokinase: cloning, expression and excretion by Escherichia coli. Proceedings of the National Academy of Sciences, USA, 81: 3557-3561.
7. Maniatis T, Fritsch EF & Sambrook J (1989). Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York.
8. Müller J, Reinert H & Malke H (1989). Streptokinase mutations relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild-type skc gene. Journal of Bacteriology, 171: 2202-2208.
9. Jackson K, Malke H, Gerlach D, Ferreti J & Tang J (1986). Active streptokinase from the cloned gene in Streptococcus sanguis is without the carboxyl-terminal 32 residues. Biochemistry, 25: 108-114.
10. Beckwith J & Ferro-Novick S (1986). Genetic studies on protein export in bacteria. Current Topics in Microbiology and Immunology, 125: 5-27.
11. Yarzábal A, Bastidas M, Avilán L, Cruz J & Puig J (1997). Induction conditions for maximizing recombinant staphylokinase expression in Escherichia coli. Biotechnology Letters, 19: 633-637.
12. Rodríguez P, Fuentes P, Barro M, Alvarez J, Munõz E, Collen D & Lijnen R (1995). Structural domains of straptokinase involved in the interaction with plasminogen. European Journal of Biochemistry, 229: 83-90.