Two streptokinase genes are expressed with different solubility in Escherichia coli W3110

Biotechnology Letters

Volumn 21, Issue 12, 1999, Pages 1119-1123

  Pupo, Elder ^a   Baghbaderani, Behnam A ^b   Lugo, Victoria ^a   Fernández, Julio ^a   Páez, Rolando ^a   Torréns, Isis ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Escherichia coli; Purification; Solubility; Streptokinase

Indexed keywords

CELL PROTEIN; STREPTOKINASE;

ARTICLE; CELL GROWTH; CELL INCLUSION; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; PURIFICATION; SOLUBILITY;

ESCHERICHIA COLI; STREPTOCOCCUS EQUISIMILIS;

EID: 0033369368     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005661724123     Document Type: Article

References (13)

1. Avilan L, Yarzabal A, Jurgensen C, Bastidas M, Cruz J, Puig J (1997) Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli. Braz. J. Med. Biol. Res. 30: 1427-1430.
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Biochemistry 72: 248-254.
3. Campos M, Ortega M, Padron G, Estrada MP, de la Fuente J, Herrera L (1991) Cloning of coliphage T4 gene pseT and high level synthesis of polynucleotide kinase in Escherichia coli. Gene 101: 127-131.
4. Collen D, Lijnen HR (1991) Basic and clinical aspects of fibrinolysis and thrombolysis. Blood 78: 3114-3124.
5. Estrada MP, Hernández L, Pérez A, Rodríguez P, Serrano R, Rubiera R, Pedraza A, Padrón G, Antuch W, de la Fuente J, Herrera L (1992) High level expression of streptokinase in Escherichia coli. Biotechnology 10: 1138-1142.
6. Hernández L, Rodríguez P, Castro A, Serrano R, Rodríguez MP, Ruviera R, Estrada MP, de la Fuente J, Herrera L (1990) Determination of streptokinase activity by quantitative assay. Biotecnología Aplicada 7: 153-160.
7. Lee SH, Kim IC, Bae KH, Byun SM (1997) Enhanced production and secretion of streptokinase into extracellular medium in Escherichia coli by removal of 13 N-terminal amino acids. Biotechnol. Lett. 19: 151-154.
8. Malke H, Roe B, Ferreti JJ (1985) Nucleotide sequence of the streptokinase gene from Streptococcus equisimilis H46A. Gene 34: 357-362.
9. Müller J, Reinert H, Malke H (1989) Streptokinase mutation relieving Escherichia coli K-12 (prlA4) of detriments caused by the wild type skc gene. J. Bacteriol. 171: 2202-2208.
10. Reed GL, Kussie P, Parhami-Seren B (1993) A functional analysis of the antigenicity of streptokinase using monoclonal antibody and recombinant streptokinase fragments. J. Immunol. 150: 4407-4415.
11. Reddy KNN et al. (1980) Mechanism of activation of human plasminogen by streptokinase. In: Kline DL, Reddy KNN, eds. Fibrinolysis. Boca Raton, FL: CRC, p. 71.
12. Towbin HT, Stahelin T, Gordon J (1979) Electrophoretic transfer of rpoteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
13. Young KC, Shi GY, Chang YF, Chang BI, Chang LC, Lai MD, Chuang WJ, Wu HL (1995) Interaction of streptokinase and plasminogen. J. Biol. Chem. 270: 29601-29606.