Expression and purification of BmrI restriction endonuclease and its N-terminal cleavage domain variants

Protein Expression and Purification

Volumn 58, Issue 1, 2008, Pages 42-52

  Bao, Yongming ^a   Higgins, Lauren ^a   Zhang, Penghua ^a   Chan, Siu hong ^a   Laget, Sophie ^a   Sweeney, Suzanne ^a   Lunnen, Keith ^a   Xu, Shuang yong ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

BmrI; Chimeric endonuclease; Cleavage domain; EDTA resistant nuclease; Enzyme purification

Indexed keywords

HYBRID PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS MEGATERIUM; CHEMISTRY; DNA CLEAVAGE; DNA REPAIR; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

AMINO ACID SEQUENCE; BACILLUS MEGATERIUM; CLONING, MOLECULAR; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA CLEAVAGE; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOS RESPONSE (GENETICS); TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MESORHIZOBIUM;

EID: 38349121904     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.11.002     Document Type: Article

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