Purification and characterization of an acid deoxyribonuclease from the cultured mycelia of Cordyceps sinensis

Journal of Biochemistry and Molecular Biology

Volumn 37, Issue 4, 2004, Pages 466-473

  Ye, MaoQing ^a   Hu, Zheng ^b   Fan, Ying ^a   He, Ling ^a   Xia, FuBao ^b   Zou, GuoLin ^a  

^a WUHAN UNIVERSITY   (China)

^b Hubei Industry University   (China)

Author keywords

Acid DNase; Cordyceps sinensis; Endonuclease

Indexed keywords

CATION; DEOXYRIBONUCLEASE II; DOUBLE STRANDED DNA; MAGNESIUM CHLORIDE; POTASSIUM CHLORIDE; SINGLE STRANDED DNA; ZINC CHLORIDE;

ANION EXCHANGE; ARTICLE; CORDYCEPS; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL FILTRATION; HYDROLYSIS; ISOELECTRIC POINT; MYCELIUM; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; TEMPERATURE DEPENDENCE;

CORDYCEPS; CORDYCEPS SINENSIS; FUNGI;

EID: 4444248383     PISSN: 12258687     EISSN: None     Source Type: Journal    
DOI: 10.5483/bmbrep.2004.37.4.466     Document Type: Article

References (36)

1. Anai, M., Muta, A. and Umeno, M. (1983) Purification and properties of an acid deoxyribonuclease from small intestinal mucosa. J. Biochem. 94, 339-344.
2. Appierto, V., Bardella, L., Vijayasarathy, C., Avadhani, N., Sgaramella, V. and Biunno, I. (1997) Functional characterization of human DNase-like protein encoded by a gene positioned in Xq28. Gene 188, 119-122.
3. Baker, K.P., Baron, W.F., Henzel, W.J. and Spencer, S.A. (1998) Molecular cloning and characterization of human and murine DNase II. Gene 215, 281-289.
4. Barry, M. A. and Eastman, A. (1993) Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch. Biochem. Biophys. 300, 440-450.
5. Bernardi, G. (1971) Spleen acid deoxyribonuclease. in The Enzymes, Boyer, P. D. (ed.), pp. 271-287, Academic Press, New York, USA.
6. Bernardi, G., Appella, E. and Zito, R. (1965) Studies on acid deoxyribonuclease. III. Physical and chemical properties of hog spleen acid deoxyribonuclease. Biochemisty 4, 1725-1729.
7. Blank, A., Sugiyama, R. H. and Dekker, C. A. (1982) Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of aqueous isopropanol to remove detergent from gels. Anal. Biochem. 120, 267-275.
8. Campbell, V. W. and Jackson, D. A. (1980) The effect of divalent cations on the mode of action of DNase I: The initial reaction products produced from covalently closed circular DNA. J. Biol. Chem. 255, 3726-3735.
9. Catcheside, D.G. and Holmes, B. (1947) The action of enzymes on chromosomes. Symp. Soc. Exp. Biol. 1, 225-231.
10. Chen, L.Y., Ho, H. C., Tsai, Y. C. and Liao, T. H. (1993) Deoxyribonuclease of Syncephalastrum racemosum enzymatic properties and molecular structure. Arch. Biochem. Biophys. 303, 51-56.
11. Conneely, A.M., Campbell, G.R., and Winder, F.G. (1976) Purification of a deoxyribonuclease from Aspergillus nidulans. Biochem. Soc. Trans. 4, 879-881.
12. Cunningham, L. and Laskowski, M. (1953) Presence of two different desoxyribonucleodepolymerases in veal kidney. Biochem. Biophys. Acta. 11, 590-591.
13. Deshpande, R. A., Kumar, A. R., Khan, M. I. and Shankar, V. (2001) Ribonuclease rs from Rhizopus stolonifer: lowering of optimum temperature in the presence of urea. Biochim. Biophys. Acta 1545, 13-19.
14. Dulaney, J. T. and Touster, O. (1972) Isolation of deoxyribonuclease II of rat liver lysosomes. J. Biol. Chem. 241, 1424-1432.
15. Harosh, I., Binninger, D. M., Harris, P. V., Mezzina, M. and Boyd, J. B. (1991) Mechanism of action of deoxyribonuclease II from human lymphoblasts. Eur. J. Biochem. 202, 479-484.
16. Hsu, T. H., Shiao, L. H., Hsieh, C. Y. and Chang, D. M. (2002) A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom DongChongXiaCao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis. Food Chemistry 78, 463-469.
17. Ikeda, S. and Takata N. (2002) Deoxyribonuclease II purified from Euglena gracilis SM-ZK, a chloroplast-lacking mutant: comparison with porcine with porcine spleen deoxyribonuclease II. Comp. Biochem. and physiol. 131, 519-525.
18. Ito, K., Minamiura, N. and Yamamoto, T. (1984) Human Urine DNase I: Immunological identity with human pancreatic DNase I, and enzymic and proteochemical properties of the enzyme. J. Biochem. 95, 1399-1406.
19. Kevin, P. B., Will, F. B., William, J. H. and Steven A. S. (1998) Molecular cloning and characterization of human and murine DNase II. Gene 215, 281-289.
20. Kitamura, A., Kouroku, Y., Onoue, M., Kimura, S., Takenouchi, M. and Sakaguchi, K. (1997) A new meiotic endonuclease from Coprinus meiocytes. Biochim. Biophys. Acta 1342, 205-216.
21. Kunitz, M. (1950) Crystalline desoxyribonuclease: Isolation and general properties; a spectrophotometric method for the measurement of desoxyribonuclease activity. J. Gen. Physiol. 33, 349-362.
22. Laemmli, U. K. and Favre, M. (1973) Gel electrophoresis of protein. J. Mol. Biol. 80, 575-599.
23. Lesca, P. (1975) Protein inhibitor of acid deoxyribonucleases; Improved purification procedure and properties. J. Biol. Chem. 254, 116-123.
24. Liao, T. H. (1985) The subunit structure and active site sequence of porcine spleen deoxyribonuclease. J. Biol. Chem. 260, 10708-10713.
25. Liao, T. H., Liao, W. C., Chang, H. C. and Lu, K. S. (1989) Deoxyribonuclease II purified from the isolated lysosomes of porcine spleen and from porcine liver homogenates. Comparison with deoxyribonuclease II purified from porcine spleen homogenates. Biochim. Biophys. Acta 1007, 15-22.
26. Lu, B. C., Wong, W., Fannibg, L. and Sakaguchi, K. (1988) Purification and characterization of an endonuclease from fruiting caps of basidiomycete Coprinus cinereus. Eur. J. Biochem. 174(4), 725-732.
27. Moore, S. (1981) Pancreatic DNase. in The Enzymes, Vol. 14, Boyer, P. D. (ed.), pp. 281-296, Academic Press, New York, USA.
28. Schomburg, D. and Salzmann, M. 1991. DNase II. in Enzyme Handbook, vol. 3. Class 3: Hydrolases, pp. 1-6, Springer, Heidelberg, Denmark.
29. SteinKraus, D.C. and Whitfield, J.B. (1994) Chinese caterpillar fungus and world record runners. American Entomologist 40, 235.
30. Sun, H., Zhao, C. G., Tong, X. and Qi, Y. P. (2003) A lectin with mycelia differentiation and antiphytovirus activities from the edible mushroom Agrocybe aegerita. J. Biochem. Mol. Biol 36, 214-222.
31. Taskeshita, H., Yasuda, T., Iida, R., Nakajima, T., Hosomi, O., Nakashima, Y., Mori, S., Nomoto, H. and Kishi, K. (1998) Identification of the three non-identical subunits constituting human deoxyribonuclease II. FEBS lett. 440, 239-242.
32. Wang, H. and Ng, T. B. (2001) Isolation of a novel deoxyribonuclease with antifungal activity from Asparagus officinalis seeds. Biochem. Biophys. Res. Commun. 289, 120-124.
33. Yamanaka, M., Tsubota, Y., Anai, M., Ishimatsu, K., Okumura, M., Katsuki, S. and Takagi, Y. (1974) Purification and properties nof acid deoxyribonuclease of human gastric mucosa and cervix uteri. J. Biol. Chem. 249, 3884-3889.
34. Yasuda, T., Nadano, D., Awazu, S. and Kishi, K. (1992) Human urine deoxyribonuclease II (DNase II) isoenzymes: a novel immunoaffinity purification, biochemical multiplicity, genetic heterogeneity and broad distribution among tissues and body fluids. Biochim. Biophys. Acta 1119, 185-193.
35. Zhu, J. S., Halpern, G. M. and Jones, K. (1998a) The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis: Part I. J. Altern Complement Med. 4, 289-303.
36. Zhu, J. S., Halpern, G. M. and Jones, K. (1998b) The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis: Part II. J. Altern Complement Med. 4, 429-457.