The stage of native biofilm formation determines the gene expression of human β-defensin-2, psoriasin, ribonuclease 7 and inflammatory mediators: A novel approach for stimulation of keratinocytes with in situ formed biofilms

Oral Microbiology and Immunology

Volumn 23, Issue 1, 2008, Pages 21-28

  Eberhard, J ^a   Menzel, N ^a   Dommisch, H ^b,c   Winter, J ^b   Jepsen, S ^b   Mutters, R ^d  

^a UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

^b UNIVERSITY HOSPITAL BONN   (Germany)

^c UNIVERSITY OF WASHINGTON   (United States)

^d UNIVERSITY HOSPITAL MARBURG   (Germany)

Author keywords

Defensins; Inflammatory mediator; Innate immunity; Keratinocytes; Oral biofilm

Indexed keywords

ARACHIDONATE 5 LIPOXYGENASE; BETA DEFENSIN 2; BIOLOGICAL MARKER; INTERLEUKIN 8; MESSENGER RNA; POLYMER; PSORIASIN; RIBONUCLEASE; RIBONUCLEASE 7; UNCLASSIFIED DRUG;

ARTICLE; BIOFILM; CELL STIMULATION; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; EPITHELIUM CELL; GENE EXPRESSION REGULATION; GINGIVAL BIOPSY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; HYPOTHESIS; IMMUNE RESPONSE; INFLAMMATION; INNATE IMMUNITY; KERATINOCYTE; MICROARRAY ANALYSIS; MOUTH MUCOSA; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECIES; UPREGULATION;

ARACHIDONATE 5-LIPOXYGENASE; BACTERIA; BETA-DEFENSINS; BIOFILMS; CALCIUM-BINDING PROTEINS; COLONY COUNT, MICROBIAL; DENTAL PLAQUE; EPITHELIAL CELLS; GENE EXPRESSION REGULATION, BACTERIAL; GINGIVA; HUMANS; IMMUNITY, NATURAL; INFLAMMATION MEDIATORS; INTERLEUKIN-8; KERATINOCYTES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBONUCLEASES; UP-REGULATION;

EID: 37349131674     PISSN: 09020055     EISSN: 1399302X     Source Type: Journal    
DOI: 10.1111/j.1399-302X.2007.00385.x     Document Type: Article

References (35)

1. Chadebech P, Goidin D, Jacquet C, Viac J, Schmitt D, Staquet MJ. Use of human reconstructed epidermis to analyze the regulation of beta-defensin hBD-1, hBD-2, and hBD-3 expression in response to LPS. Cell Biol Toxicol 2003 : 19 : 313 324.
2. Dale BA. Periodontal epithelium: a newly recognized role in health and disease. Periodontol 2000 2002 : 30 : 70 78.
3. Dale BA, Kimball JR, Krisanaprakornkit S et al. Localized antimicrobial peptide expression in human gingiva. J Periodontal Res 2001 : 36 : 285 294.
4. Dinulos JG, Mentele L, Fredericks LP, Dale BA, Darmstadt GL. Keratinocyte expression of human beta defensin 2 following bacterial infection: role in cutaneous host defense. Clin Diagn Lab Immunol 2003 : 10 : 161 166.
5. Dommisch H, Acil Y, Dunsche A, Winter J, Jepsen S. Differential gene expression of human beta-defensins (hBD-1, -2, -3) in inflammatory gingival diseases. Oral Microbiol Immunol 2005 : 20 : 186 190.
6. Duits LA, Rademaker M, Ravensbergen B et al. Inhibition of hBD-3, but not hBD-1 and hBD-2, mRNA expression by corticosteroids. Biochem Biophys Res Commun 2001 : 280 : 522 525.
7. Dunsche A, Acil Y, Siebert R, Harder J, Schroder JM, Jepsen S. Expression profile of human defensins and antimicrobial proteins in oral tissues. J Oral Pathol Med 2001 : 30 : 154 158.
8. Eberhard J, Jepsen S, Pohl L, Albers HK, Acil Y. Bacterial challenge stimulates formation of arachidonic acid metabolites by human keratinocytes and neutrophils in vitro. Clin Diagn Lab Immunol 2002 : 9 : 132 137.
9. Fellermann K, Stange DE, Schaeffeler E et al. A chromosome 8 gene-cluster polymorphism with low human beta-defensin 2 gene copy number predisposes to Crohn disease of the colon. Am J Hum Genet 2006 : 79 : 439 448.
10. Feucht EC, DeSanti CL, Weinberg A. Selective induction of human beta-defensin mRNAs by Actinobacillus actinomycetemcomitans in primary and immortalized oral epithelial cells. Oral Microbiol Immunol 2003 : 18 : 359 363.
11. Ganz T. Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 2003 : 3 : 710 720.
12. Glaser R, Harder J, Lange H, Bartels J, Christophers E, Schroder JM. Antimicrobial psoriasin (S100A7) protects human skin from Escherichia coli infection. Nat Immunol 2005 : 6 : 57 64.
13. Harder J, Meyer-Hoffert U, Teran LM, et al. Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 2000 : 22 : 714 721.
14. Harder J, Schroder JM. RNase 7, a novel innate immune defense antimicrobial protein of healthy human skin. J Biol Chem 2002 : 277 : 46779 46784.
15. Harder J, Schroder JM. Psoriatic scales: a promising source for the isolation of human skin-derived antimicrobial proteins. J Leukoc Biol 2005 : 77 : 476 486.
16. Hollox EJ, Armour JA, Barber JC. Extensive normal copy number variation of a beta-defensin antimicrobial-gene cluster. Am J Hum Genet 2003 : 73 : 591 600.
17. Joly S, Maze C, McCray PB Jr., Guthmiller JM. Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. J Clin Microbiol 2004 : 42 : 1024 1029.
18. Joly S, Organ CC, Johnson GK, McCray PB Jr., Guthmiller JM. Correlation between beta-defensin expression and induction profiles in gingival keratinocytes. Mol Immunol 2005 : 42 : 1073 1084.
19. Jurevic RJ, Bai M, Chadwick RB, White TC, Dale BA. Single-nucleotide polymorphisms (SNPs) in human beta-defensin 1: high-throughput SNP assays and association with Candida carriage in type I diabetics and nondiabetic controls. J Clin Microbiol 2003 : 41 : 90 96.
20. Kimball JR, Nittayananta W, Klausner M, Chung WO, Dale BA. Antimicrobial barrier of an in vitro oral epithelial model. Arch Oral Biol 2006 : 51 : 775 783.
21. Krisanaprakornkit S, Kimball JR, Weinberg A, Darveau RP, Bainbridge BW, Dale BA. Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect Immun 2000 : 68 : 2907 2915.
22. Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA. Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun 1998 : 66 : 4222 4228.
23. Linzmeier RM, Ganz T. Human defensin gene copy number polymorphisms: comprehensive analysis of independent variation in alpha- and beta-defensin regions at 8p22-p23. Genomics 2005 : 86 : 423 430.
24. Liu AY, Destoumieux D, Wong AV, et al. Human beta-defensin-2 production in keratinocytes is regulated by interleukin-1, bacteria, and the state of differentiation. J Invest Dermatol 2002 : 118 : 275 281.
25. Marone M, Mozzetti S, De Ritis D, Pierelli L, Scambia G. Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 2001 : 3 : 19 25.
26. Marshall RI. Gingival defensins: linking the innate and adaptive immune responses to dental plaque. Periodontol 2000 2004 : 35 : 14 20.
27. Meyer-Hoffert U, Wichmann N, Schwichtenberg L, White PC, Wiedow O. Supernatants of Pseudomonas aeruginosa induce the Pseudomonas-specific antibiotic elafin in human keratinocytes. Exp Dermatol 2003 : 12 : 418 425.
28. Nishimura E, Eto A, Kato M et al. Oral streptococci exhibit diverse susceptibility to human beta-defensin-2: antimicrobial effects of hBD-2 on oral streptococci. Curr Microbiol 2004 : 48 : 85 87.
29. Noguchi T, Shiba H, Komatsuzawa H et al. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans. Inflammation 2003 : 27 : 341 349.
30. Quirynen M, Mongardini C, Lambrechts P et al. A long-term evaluation of composite-bonded natural/resin teeth as replacement of lower incisors with terminal periodontitis. J Periodontol 1999 : 70 : 205 212.
31. Ramberg P, Sekino S, Uzel NG, Socransky S, Lindhe J. Bacterial colonization during de novo plaque formation. J Clin Periodontol 2003 : 30 : 990 995.
32. Socransky SS, Manganiello AD, Propas D, Oram V, van Houte J. Bacteriological studies of developing supragingival dental plaque. J Periodontal Res 1977 : 12 : 90 106.
33. Takahashi A, Wada A, Ogushi K et al. Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein. FEBS Lett 2001 : 508 : 484 488.
34. Tanner J, Robinson C, Soderling E, Vallittu P. Early plaque formation on fibre-reinforced composites in vivo. Clin Oral Invest 2005 : 9 : 154 160.
35. Vankeerberghen A, Nuytten H, Dierickx K, Quirynen M, Cassiman JJ, Cuppens H. Differential induction of human beta-defensin expression by periodontal commensals and pathogens in periodontal pocket epithelial cells. J Periodontol 2005 : 76 : 1293 1303.