Expression, purification, and characterization of a structurally disordered and functional C-terminal autoinhibitory domain (AID) of the 70 kDa 40S ribosomal protein S6 kinase-1 (S6K1)

Protein Expression and Purification

Volumn 57, Issue 2, 2008, Pages 271-279

  Ragan, Timothy J ^a   Ross, Duncan B ^a   Keshwani, Malik M ^b   Harris, Thomas K ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b Department of Biology   (United States)

Author keywords

Autoinhibition; Custom gene synthesis; Disordered proteins; Intrinsic disorder; Minimal media; PDK1; Phosphoinositide dependent protein kinase 1; Proteolysis inhibition

Indexed keywords

MUTANT PROTEIN; PHOSPHOTHREONINE; PROTEIN SERINE THREONINE KINASE; PYRUVATE DEHYDROGENASE (ACETYL TRANSFERRING) KINASE; PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING) KINASE; RECEPTOR LIKE PROTEIN TYROSINE PHOSPHATASE; S6 KINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEOTIDE SEQUENCE; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYSIS; ESCHERICHIA COLI; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTANT PROTEINS; PHOSPHOTHREONINE; PROTEIN STRUCTURE, TERTIARY; PROTEIN-SERINE-THREONINE KINASES; RECEPTOR-LIKE PROTEIN TYROSINE PHOSPHATASES, CLASS 2; RIBOSOMAL PROTEIN S6 KINASES, 70-KDA;

EID: 37349113787     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.09.014     Document Type: Article

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