6-Deoxyerythronolide B production through chromosomal localization of the deoxyerythronolide B synthase genes in E. coli

Metabolic Engineering

Volumn 10, Issue 1, 2008, Pages 33-38

  Wang, Yong ^a   Pfeifer, Blaine A ^a  

^a Tufts University   (United States)

Author keywords

6 Deoxyerythronolide B; Chromosomal engineering; Deoxyerythronolide B synthase; E. coli

Indexed keywords

ANTIBIOTIC ERYTHROMYCIN; CHROMOSOMAL ENGINEERING; DEOXYERYTHRONOLIDE;

ANTIBIOTICS; BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; METABOLISM;

GENES;

6 DEOXYERYTHRONOLIDE B; DEOXYERYTHRONOLIDE B; DEOXYERYTHRONOLIDE B SYNTHASE; ERYTHROMYCIN DERIVATIVE; ERYTHRONOLIDE B; POLYKETIDE; PROPIONYL COENZYME A CARBOXYLASE; UNCLASSIFIED DRUG;

ARTICLE; BAP1 GENE; CHROMOSOMAL LOCALIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI YW9; GENE; GENE EXPRESSION REGULATION; GENE LOCATION; GENETIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE MEASUREMENT;

CHROMOSOMES, BACTERIAL; ERYTHROMYCIN; ESCHERICHIA COLI; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; INDICATORS AND REAGENTS; PLASMIDS; POLYKETIDE SYNTHASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 37349092706     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2007.09.002     Document Type: Article

References (20)

1. Datsenko K.A., and Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97 12 (2000) 6640-6645
2. Eppelmann K., Doekel S., and Marahiel M.A. Engineered biosynthesis of the peptide antibiotic bacitracin in the surrogate host Bacillus subtilis. J. Biol. Chem. 276 37 (2001) 34824-34831
3. Frykman S., Leaf T., Carreras C., and Licari P. Precursor-directed production of erythromycin analogs by Saccharopolyspora erythraea. Biotechnol. Bioeng. 76 4 (2001) 303-310
4. Gross F., Ring M.W., Perlova O., Fu J., Schneider S., Gerth K., Kuhlmann S., Stewart A.F., Zhang Y., and Muller R. Metabolic engineering of Pseudomonas putida for methylmalonyl-CoA biosynthesis to enable complex heterologous secondary metabolite formation. Chem. Biol. 13 12 (2006) 1253-1264
5. Hamilton C.M., Aldea M., Washburn B.K., Babitzke P., and Kushner S.R. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171 9 (1989) 4617-4622
6. Kao C.M., Katz L., and Khosla C. Engineered biosynthesis of a complete macrolactone in a heterologous host. Science 265 5171 (1994) 509-512
7. Lambalot R.H., Gehring A.M., Flugel R.S., Zuber P., LaCelle M., Marahiel M.A., Reid R., Khosla C., and Walsh C.T. A new enzyme superfamily-the phosphopantetheinyl transferases. Chem. Biol. 3 11 (1996) 923-936
8. Lau J., Tran C., Licari P., and Galazzo J. Development of a high cell-density fed-batch bioprocess for the heterologous production of 6-deoxyerythronolide B in Escherichia coli. J. Biotechnol. 110 1 (2004) 95-103
9. Link A.J., Phillips D., and Church G.M. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179 20 (1997) 6228-6237
10. Menzella H.G., Reisinger S.J., Welch M., Kealey J.T., Kennedy J., Reid R., Tran C.Q., and Santi D.V. Redesign, synthesis and functional expression of the 6-deoxyerythronolide B polyketide synthase gene cluster. J. Ind. Microbiol. Biotechnol. 33 1 (2006) 22-28
11. Murli S., Kennedy J., Dayem L.C., Carney J.R., and Kealey J.T. Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production. J. Ind. Microbiol. Biotechnol. 30 8 (2003) 500-509
12. Mutka S.C., Carney J.R., Liu Y., and Kennedy J. Heterologous production of epothilone C and D in Escherichia coli. Biochemistry 45 4 (2006) 1321-1330
13. Peiru S., Menzella H.G., Rodriguez E., Carney J., and Gramajo H. Production of the potent antibacterial polyketide erythromycin C in Escherichia coli. Appl. Environ. Microbiol. 71 5 (2005) 2539-2547
14. Pfeifer B.A., and Khosla C. Biosynthesis of polyketides in heterologous hosts. Microbiol. Mol. Biol. Rev. 65 1 (2001) 106-118
15. Pfeifer B.A., Admiraal S.J., Gramajo H., Cane D.E., and Khosla C. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli. Science 291 5509 (2001) 1790-1792
16. Pfeifer B., Hu Z., Licari P., and Khosla C. Process and metabolic strategies for improved production of Escherichia coli-derived 6-deoxyerythronolide B. Appl. Environ. Microbiol. 68 7 (2002) 3287-3292
17. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
18. Sosio M., Giusino F., Cappellano C., Bossi E., Puglia A.M., and Donadio S. Artificial chromosomes for antibiotic-producing actinomycetes. Nat. Biotechnol. 18 3 (2000) 343-345
19. Studier F.W., and Moffatt B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189 1 (1986) 113-130
20. Yu D., Ellis H.M., Lee E.C., Jenkins N.A., Copeland N.G., and Court D.L. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97 11 (2000) 5978-5983