Process and metabolic strategies for improved production of Escherichia coli-derived 6-deoxyerythronolide B

Applied and Environmental Microbiology

Volumn 68, Issue 7, 2002, Pages 3287-3292

  Pfeifer, Blaine ^a   Hu, Zhihao ^a   Licari, Peter ^a   Khosla, Chaitan ^a  

^a Stanford University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; FERMENTATION; METABOLITES; ROBUSTNESS (CONTROL SYSTEMS);

FEASIBILITY;

ESCHERICHIA COLI;

6 DEOXYERYTHRONOLIDE B; ERYTHROMYCIN; ERYTHRONOLIDE B; POLYKETIDE; RECOMBINANT PROTEIN; THIOL ESTER HYDROLASE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOSYNTHESIS; CELL DENSITY; ESCHERICHIA COLI; FED BATCH FERMENTATION; METABOLIC ENGINEERING; NONHUMAN; PROTEIN SYNTHESIS; SACCHAROPOLYSPORA;

ERYTHROMYCIN; ESCHERICHIA COLI; FERMENTATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA;

EID: 0036306047     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.7.3287-3292.2002     Document Type: Article

References (14)

1. Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae
2. Harnessing the biosynthetic code: Combinations, permutations, and mutations
3. Mechanism and specificity of the terminal thioesterase domain from the erythromycin polyketide synthase
4. Role of type II thioesterases: Evidence for removal of short acyl chains produced by aberrant decarboxylation of chain extender units
5. Dissection of IncP conjugative plasmid transfer: Definition of the transfer region Tra2 by mobilization of the Tra1 region in trans
6. Enhancing the atom economy of polyketide biosynthetic processes through metabolic engineering
7. Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products
8. Improved erythromycin production in a genetically engineered industrial strain of Saccharopolyspora erythraea
9. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli
10. Biosynthesis of polyketides in heterologous hosts
11. Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases
12. Elucidating the mechanism of chain termination switching in the picromycin/methymycin polyketide synthase
13. The effect of organic nitrogen and glucose on the production of recombinant human insulin-like growth factor in high cell density Escherichia coli fermentations
14. Organization of a cluster of erythromycin genes in Saccharopolyspora erythraea