Precursor-directed production of erythromycin analogs by Saccharopolyspora erythraea

Biotechnology and Bioengineering

Volumn 76, Issue 4, 2001, Pages 303-310

  Frykman, Scott ^a   Leaf, Timothy ^a   Carreras, Chris ^a   Licari, Peter ^a  

^a KOSAN BIOSCIENCES INC   (United States)

Author keywords

Chemobiosynthesis; Diketide; Erythraea; Erythromycin; Polyketide; Thioester

Indexed keywords

AMINES; BIOSYNTHESIS; DEGRADATION; SUBSTITUTION REACTIONS;

RECOMBINANT STRAIN;

ENZYMES;

CYSTEAMINE DERIVATIVE; DIKETIDE N ACETYLCYSTEAMINE; ERYTHROMYCIN DERIVATIVE; ERYTHRONOLIDE DERIVATIVE; KETONE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOSYNTHESIS; DRUG SYNTHESIS; ENANTIOMER; FATTY ACID OXIDATION; NONHUMAN; PRECURSOR; SACCHAROPOLYSPORA; STREPTOMYCES ERYTHRAEUS;

ANTI-BACTERIAL AGENTS; CELL DIVISION; DOSE-RESPONSE RELATIONSHIP, DRUG; ERYTHROMYCIN; ESTERS; KINETICS; MODELS, CHEMICAL; OXYGEN; PROTEIN BINDING; SACCHAROPOLYSPORA; STREPTOMYCES; TIME FACTORS;

SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA; STREPTOMYCES; STREPTOMYCES COELICOLOR;

EID: 0035829834     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10086     Document Type: Article

References (12)

1. Production of 6-deoxy-13-cyclopropyl-erythromycin B by Saccharopolyspora erythraea NRRL 18643
2. Precursor-directed biosynthesis of erythromycin analogs by an engineered polyketide synthase
3. Evidence for two catalytically independent clusters of active sites in a functional modular polyketide synthase
4. Identification of novel erythromycin derivatives in mother liquor concentrations of Streptomyces erythraeus
5. Precursor-directed biosynthesis of 6-deoxyerythronolide B analogs in Streptomyces coelicolor: Understanding precursor effects
6. Biosynthetic incorporation of labeled tetraketide intermediates into dehydrocurvularin, a phytotoxin from Alternaria cinerariae, with assistance of β-oxidation inhibitors
7. Engineering broader specificity into an antibiotic-producing polyketide synthase
8. Remarkably broad substrate specificity of a modular polyketide synthase in a cell-free system
9. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): Inhibition of fatty acid β-oxidation by acrylic acid
10. Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis
11. Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production
12. Evaluating precursor-directed biosynthesis towards novel erythromycins through in vitro studies on a bimodular polyketide synthase