Lysyl-tRNA synthetase interacts with EF1α, aspartyl-tRNA synthetase and p38 in vitro

Biochemical and Biophysical Research Communications

Volumn 365, Issue 4, 2008, Pages 718-723

  Guzzo, Catherine M ^a   Yang, David C H ^a  

^a GEORGETOWN UNIVERSITY   (United States)

Author keywords

Amino terminal extensions; Aminoacylation; EF1 ; Enzyme interactions; Lysyl tRNA synthetase; Multi enzyme complex; p38; Supramolecular assembly

Indexed keywords

AMINO ACID TRANSFER RNA LIGASE; ASPARTIC ACID TRANSFER RNA; CYTOKINE; ELONGATION FACTOR 1ALPHA; GUANOSINE TRIPHOSPHATE; LYSINE TRANSFER RNA; LYSINE TRANSFER RNA LIGASE; PARKIN; SCAFFOLD PROTEIN; SYNAPTOPHYSIN; SYNTHETASE;

AMINOACYLATION; ARTICLE; BINDING AFFINITY; IN VITRO STUDY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; STOICHIOMETRY;

LYSINE-TRNA LIGASE; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PEPTIDE ELONGATION FACTOR 1; PROTEIN INTERACTION MAPPING; RNA, TRANSFER, AMINO ACYL; TWO-HYBRID SYSTEM TECHNIQUES;

MAMMALIA;

EID: 36849031896     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.11.028     Document Type: Article

References (24)

1. Ray P.S., Arif A., and Fox P.L. Macromolecular complexes as depots for releasable regulatory proteins. Trends Biochem. Sci. 32 (2007) 158-164
2. Crane-Robinson C., Dragan A.I., and Privalov P.L. The extended arms of DNA-binding domains: a tale of tails. Trends Biochem. Sci. 31 (2006) 547-552
3. Mirande M., Kellermann O., and Waller J.P. Macromolecular complexes from sheep and rabbit containing seven aminoacyl-tRNA synthetases. J. Biol. Chem. 257 (1982) 11049-11055
4. Rho S.B., Lee K.H., Kim J.W., Shiba K., Jo Y.J., and Kim S. Interaction between human tRNA synthetases involves repeated sequence elements. Proc. Natl. Acad. Sci. USA 93 (1996) 10128-10133
5. Robinson J.C., Kerjan P., and Mirande M. Macromolecular assemblage of aminoacyl-tRNA synthetases. J. Mol. Biol. 304 (2000) 983-994
6. Wolfe C.L., Warrington J.A., Treadwell L., and Norcum M.T. A three-dimensional working model of the multienzyme complex of aminoacyl-tRNA synthetases based on electron microscopic placements of tRNA and proteins. J. Biol. Chem. 280 (2005) 38870-38878
7. Vellekamp G., and Deutscher M.P. A basic NH2-terminal extension of rat liver arginyl-tRNA synthetase required for its association with high molecular weight complexes. J. Biol. Chem. 262 (1987) 9927-9930
8. Cirakoglu B., Mirande M., and Waller J.P. A model for the structural organization of aminoacyl-tRNA synthetases in mammalian cells. FEBS Lett. 183 (1985) 185-190
9. Jacobo-Molina A., Peterson R., and Yang D.C. cDNA sequence, predicted primary structure, and evolving amphiphilic helix of human aspartyl-tRNA synthetase. J. Biol. Chem. 264 (1989) 16608-16612
10. Shiba K., Stello T., Motegi H., Noda T., Musier-Forsyth K., and Schimmel P. Human lysyl-tRNA synthetase accepts nucleotide 73 variants and rescues Escherichia coli double-defective mutant. J. Biol. Chem. 272 (1997) 22809-22816
11. Kim J.Y., Kang Y.S., Lee J.W., Kim H.J., Ahn Y.H., Park H., Ko Y.G., and Kim S. p38 is essential for the assembly and stability of macromolecular tRNA synthetase complex: implications for its physiological significance. Proc. Natl. Acad. Sci. USA 99 (2002) 7912-7916
12. Quevillon S., Agou F., Robinson J.C., and Mirande M. The p43 component of the mammalian multi-synthetase complex is likely to be the precursor of the endothelial monocyte-activating polypeptide II cytokine. J. Biol. Chem. 272 (1997) 32573-32579
13. Kim M.J., Park B.J., Kang Y.S., Kim H.J., Park J.H., Kang J.W., Lee S.W., Han J.M., Lee H.W., and Kim S. Downregulation of FUSE-binding protein and c-myc by tRNA synthetase cofactor p38 is required for lung cell differentiation. Nat. Genet. 34 (2003) 330-336
14. Corti O., Hampe C., Koutnikova H., Darios F., Jacquier S., Prigent A., Robinson J.C., Pradier L., Ruberg M., Mirande M., Hirsch E., Rooney T., Fournier A., and Brice A. The p38 subunit of the aminoacyl-tRNA synthetase complex is a Parkin substrate: linking protein biosynthesis and neurodegeneration. Hum. Mol. Genet. 12 (2003) 1427-1437
15. Dang C.V., and Yang D.C.H. Disassembly and gross structure of particulate aminoacyl-tRNA synthetases from rat liver. J. Biol. Chem. 254 (1979) 5350-5356
16. Francin M., Kaminska M., Kerjan P., and Mirande M. The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain. J. Biol. Chem. 277 (2002) 1762-1769
17. Francin M., and Mirande M. Identity elements for specific aminoacylation of a tRNA by mammalian lysyl-tRNA synthetase bearing a non-specific tRNA-interacting factor. Biochemistry 45 (2006) 10153-10160
18. Reed V.S., Wastney M., and Yang D.C.H. Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1α. J. Biol. Chem. 269 (1994) 32932-32936
19. Agou F., and Mirande M. Aspartyl-tRNA synthetase from rat: in vitro functional analysis of its assembly into the multisynthetase complex. Eur. J. Biochem. 243 (1997) 259-267
20. Wang T., Evdokimov E., Yiadom K., Yan Z., Chock P.B., and Yang D.C. Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli. Protein Expr. Purif. 30 (2003) 140-149
21. Guzzo C.M., and Yang D.C. Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E. coli. Protein Expr. Purif. 54 (2007) 166-175
22. Escalante C., and Yang D.C. Expression of human aspartyl-tRNA synthetase in Escherichia coli: functional analysis of the N-terminal putative amphiphilic helix. J. Biol. Chem. 268 (1993) 6014-6023
23. Venema R.C., and Traugh J.A. Protein kinase C phosphorylates glutamyl-tRNA synthetase in rabbit reticulocytes stimulated by tumor promoting phorbol esters. J. Biol. Chem. 266 (1991) 5298-5302
24. Pendergast A.M., and Traugh J. Alteration of aminoacyl-tRNA synthetase activities by phosphorylation with casein kinase I. J. Biol. Chem. 260 (1985) 11769-11774