Evaluation of the Staphylococcus aureus class C nonspecific acid phosphatase (SapS) as a reporter for gene expression and protein secretion in gram-negative and gram-positive bacteria

Applied and Environmental Microbiology

Volumn 73, Issue 22, 2007, Pages 7232-7239

  Du Plessis, Erika ^a   Theron, Jacques ^b   Berger, Eldie ^a   Louw, Maureen ^a  

^a DSI CSIR Nanotechnology Innovation Centre   (South Africa)

^b UNIVERSITY OF PRETORIA   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PROTEASES; HETEROLOGOUS PROTEINS; REPORTER GENES; TRANSLATIONAL FUSIONS;

ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; PHOSPHATES; PROTEINS;

BACTERIA;

ACID PHOSPHATASE; ALKALINE PROTEINASE; PROTEIN SAPS; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

BACTERIUM; BIOASSAY; ENZYME ACTIVITY; GENE EXPRESSION; PROTEIN;

ARTICLE; BACILLUS; BACILLUS HALODURANS; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; COST EFFECTIVENESS ANALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; LACZ GENE; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; QUANTITATIVE ASSAY; REGULATORY SEQUENCE; REPORTER GENE; SAPS GENE; STAPHYLOCOCCUS AUREUS; ZYMOGRAPHY;

ACID PHOSPHATASE; BACILLUS SUBTILIS; BACTERIOLOGICAL TECHNIQUES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENETIC TECHNIQUES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; STAPHYLOCOCCUS AUREUS;

BACILLUS HALODURANS; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

EID: 36648999378     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01030-07     Document Type: Article

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