Visualization of differential gene expression by improved cyan fluorescent protein and yellow fluorescent protein production in Bacillus subtilis

Applied and Environmental Microbiology

Volumn 70, Issue 11, 2004, Pages 6809-6815

  Veening, Jan Willem ^a   Smits, Wiep Klaas ^a   Hamoen, Leendert W ^a,b   Jongbloed, Jan D H ^a   Kuipers, Oscar P ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CELL CULTURE; FLUORESCENCE; PROTEINS;

CYAN FLUORESCENT PROTEINS (CFP); EUKARYOTIC CONDON BIAS; YELLOW FLUORESCENT PROTEINS (YFP);

GENES;

AMINO ACID; BACTERIAL PROTEIN; CYAN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN COMGA; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

BACTERIUM;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; CLONING VECTOR; CODON; EUKARYOTIC CELL; GENE EXPRESSION; GENE EXPRESSION REGULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; SPOIIA GENE; SPOROGENESIS; TRANSLATION INITIATION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CULTURE MEDIA; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION FACTORS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); EUKARYOTA; POSIBACTERIA; PROKARYOTA; UNIDENTIFIED CLONING VECTOR; VECTORS;

EID: 8744302067     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.11.6809-6815.2004     Document Type: Article

References (36)

1. Albano, M., R. Breitling, and D. A. Dubnau. 1989. Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon. J. Bacteriol. 171: 5386-5404.
2. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
3. Chung, J. D., G. Stephanopoulos, K. Ireton, and A. D. Grossraan. 1994. Gene expression in single cells of Bacillus subtilis: evidence that a threshold mechanism controls the initiation of sporulation. J. Bacteriol. 176:1977-1984.
4. Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
5. Dubnau, D. 1991. The regulation of genetic competence in Bacillus subtilis. Mol. Microbiol. 5:11-18.
6. Elowitz, M. B., A. J. Levine, E. D. Siggia, and P. S. Swain. 2002. Stochastic gene expression in a single cell. Science 297:1183-1186.
7. Errington, J. 1993. Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis. Microbiol. Rev. 57:1-33.
8. Feucht, A., and P. J. Lewis. 2001. Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis. Gene 264: 289-297.
9. Glaser, P., M. E. Sharpe, B. Raether, M. Perego, K. Ohlsen, and J. Errington. 1997. Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning. Genes Dev. 11:1160-1168.
10. Gonzalez-Pastor, J. E., E. C. Hobbs, and R. Losick. 2003. Cannibalism by sporulating bacteria. Science 301:510-513.
11. Haas, J., E. C. Park, and B. Seed. 1996. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6:315-324.
12. Haijema, B. J., J. Hahn, J. Haynes, and D. Dubnau. 2001. A ComGA-dependent checkpoint limits growth during the escape from competence. Mol. Microbiol. 40:52-64.
13. Hamoen, L. W., D. Kausche, M. A. Marahiel, D. van Sinderen, G. Venema, and P. Serror. 2003. The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK. FEMS Microbiol. Lett. 218:299-304.
14. Heim, R., and R. Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:178-182.
15. Kaltwasser, M., T. Wiegert, and W. Schumann. 2002. Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis. Appl. Environ. Microbiol. 68:2624-2628.
16. Kunst, F., et al. 1997. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390:249-256.
17. Leskela, S., V. P. Kontinen, and M. Sarvas. 1996. Molecular analysis of an operon in Bacillus subtilis encoding a novel ABC transporter with a role in exoprotein production, sporulation and competence. Microbiology 142:71-77.
18. Lewis, P. J., and A. L. Marston. 1999. GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene 227:101-110.
19. Losick, R., and P. Stragier. 1992. Crisscross regulation of cell-type-specific gene expression during development in B. subtilis. Nature 355:601-604.
20. Mascarenhas, J., J. Soppa, A. V. Strunnikov, and P. L. Graumann. 2002. Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein. EMBO J. 21:3108-3118.
21. Molle, V., M. Fujita, S. T. Jensen, P. Eichenberger, J. E. Gonzalez-Pastor, J. S. Liu, and R. Losick. 2003. The Spo0A regulon of Bacillus subtilis. Mol. Microbiol. 50:1683-1701.
22. Moszer, I., E. P. Rocha, and A. Danchin. 1999. Codon usage and lateral gene transfer in Bacillus subtilis. Curr. Opin. Microbiol. 2:524-528.
23. O'Reilly, M., and K. M. Devine. 1997. Expression of AbrB, a transition state regulator from Bacillus subtilis, is growth phase dependent in a manner resembling that of Fis, the nucleoid binding protein from Escherichia coli. J. Bacteriol. 179:522-529.
24. Ozhudak, E. M., M. Thattai, I. Kurtser, A. D. Grossman, and A. van Oudenaarden. 2002. Regulation of noise in the expression of a single gene. Nat. Genet. 31:69-73.
25. Perego, M., C. Hanstein, K. M. Welsh, T. Djavakhishvili, P. Glaser, and J. A. Hoch. 1994. Multiple protein-aspartate phosphatases provide a mechanism for the integration of diverse signals in the control of development in B. subtilis. Cell 79:1047-1055.
26. Robertson, J. B., M. Gocht, M. A. Marahiel, and P. Zuber. 1989. AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene. Proc. Natl. Acad. Sci. USA 86:8457-8461.
27. Robinson, M., R. Lilley, S. Little, J. S. Emtage, G. Yarranton, P. Stephens, A. Millican, M. Eaton, and G. Humphreys. 1984. Codon usage can affect efficiency of translation of genes in Escherichia coli. Nucleic Acids Res. 12:6663-6671.
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
29. Schmeisser, F., J. A. Brannigan, R. J. Lewis, A. J. Wilkinson, P. Youngman, and I. Barak. 2000. A new mutation in spo0A with intragenic suppressors in the effector domain. FEMS Microbiol. Lett. 185:123-128.
30. Sharp, P. M., E. Cowe, D. G. Higgins, D. C. Shields, K. H. Wolfe, and F. Wright. 1988. Codon usage patterns in Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster and Homo sapiens; a review of the considerable within-species diversity. Nucleic Acids Res. 16:8207-8211.
31. Sonenshein, A. L. 2000. Control of sporulation initiation in Bacillus subtilis. Curr. Opin. Microbiol. 3:561-566.
32. Sorensen, M. A., C. G. Kurland, and S. Pedersen. 1989. Codon usage determines translation rate in Escherichia coli. J. Mol. Biol. 207:365-377.
33. Strauch, M. A., G. B. Spiegelman, M. Perego, W. C. Johnson, D. Burbulys, and J. A. Hoch. 1989. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 8:1615-1621.
34. van Dijl, J. M., A. de Jong, H. Smith, S. Bron, and G. Venema. 1991. Non-functional expression of Escherichia coli signal peptidase I in Bacillus subtilis. J. Gen. Microbiol. 137:2073-2083.
35. van Hijum, S. A. F. T., R. J. Baerends, H. A. Karsens, A. de Jong, N. E. Kramer, C. J. Albers, J. Kok, and O. P. Kuipers. Submitted for publication.
36. Yuan, G., and S. L. Wong. 1995. Regulation of groE expression in Bacillus subtilis: the involvement of the sigma A-like promoter and the roles of the inverted repeat sequence (CIRCE). J. Bacteriol. 177:5427-5433.