Purification, cloning, and properties of α-galactosidase from Saccharopolyspora erythraea and its use as a reporter system

Applied Microbiology and Biotechnology

Volumn 67, Issue 1, 2005, Pages 91-96

  Post, David A ^a   Luebke, Vicki E ^a  

^a ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CARBON; CLONING; ENZYMES; MOLECULAR WEIGHT; PH EFFECTS; PURIFICATION;

Α-GALACTOSIDASE; MOLECULAR MASSES; OLIGONUCLEOTIDES; SACHAROPOLYSPORA ERYTHRAEA;

ENZYME KINETICS;

ERYTHROMYCIN; GLUCOPYRANOSIDE; MELIBIOSE;

BIOTECHNOLOGY; ENZYME;

AGAR MEDIUM; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE LAMBDA; CONTROLLED STUDY; ENZYME PURIFICATION; ENZYME SUBSTRATE; GENE AMPLIFICATION; GENE LIBRARY; LIQUID CULTURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OLIGONUCLEOTIDE PROBE; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; REPORTER GENE; SACCHAROPOLYSPORA;

ALPHA-GALACTOSIDASE; BACTERIAL PROTEINS; CHROMOGENIC COMPOUNDS; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; GALACTOSE; GENES, BACTERIAL; GENES, REPORTER; GLUCOSIDES; HYDROGEN-ION CONCENTRATION; KINETICS; MELIBIOSE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SACCHAROPOLYSPORA; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); SACCHAROPOLYSPORA ERYTHRAEA;

EID: 17444408484     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1764-6     Document Type: Article

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