Dexamethasone prevents transport inhibition by hypoxia in rat lung and alveolar epithelial cells by stimulating activity and expression of Na +-K+-ATPase and epithelial Na+ channels

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 293, Issue 5, 2007, Pages

  Güney, Şevin ^b   Schuler, Akelei ^a   Ott, Alexandra ^a   Höschele, Sabine ^a   Zügel, Stefanie ^a   Baloǧlu, Emel ^a   Bärtsch, Peter ^a   Mairbäurl, Heimo ^a,c  

^a UNIVERSITY HOSPITAL HEIDELBERG   (Germany)

^b GAZI UNIVERSITY FACULTY OF MEDICINE   (Turkey)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Alveolar fluid reabsorption; Hypoxic pulmonary edema; Ion transport; mRNA expression

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AMILORIDE; DEXAMETHASONE; SODIUM;

ARTICLE; CONTROLLED STUDY; DRUG EFFECT; EPITHELIUM CELL; HYPOXIA; ION TRANSPORT; LUNG ALVEOLUS EPITHELIUM; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REAL TIME POLYMERASE CHAIN REACTION; SODIUM TRANSPORT;

ANIMALS; ANOXIA; BIOLOGICAL TRANSPORT; DEXAMETHASONE; EPITHELIAL SODIUM CHANNEL; EPITHELIUM; LUNG; MALE; PULMONARY ALVEOLI; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; SODIUM-POTASSIUM-EXCHANGING ATPASE;

EID: 36348994482     PISSN: 10400605     EISSN: 15221504     Source Type: Journal    
DOI: 10.1152/ajplung.00338.2006     Document Type: Article

References (36)

1. Azzam ZS, Dumasius V, Saldias FJ, Adir Y, Sznajder JI, Factor P. Na,K-ATPase overexpression improves alveolar fluid clearance in a rat model of elevated left atrial pressure. Circulation 105: 497-501, 2002.
2. Bhattacharya J. The alveolar water gate. Am J Physiol Lung Cell Mol Physiol 286: L257-L258, 2004.
3. + channel by steroid hormones. EMBO J 13: 2177-2181, 1994.
4. 2 tension. Kidney Int 65: S79-S83, 1998.
5. Clerici C, Matthay MA. Hypoxia regulates gene expression of alveolar epithelial transport proteins. J Appl Physiol 88: 1890-1896, 2000.
6. Dada LA, Chandel NS, Ridge KM, Pedemonte C, Bertorello AM, Sznajder JI. Hypoxia-induced endocytosis of Na,K-ATPase in alveolar epithelial cells is mediated by mitochondrial reactive oxygen species and PKC-zeta. J Clin Invest 111: 1057-1064, 2003.
7. +-ATPase by cAMP and dexamethasone in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 281: L217-L230, 2001.
8. Dehler M, Zessin E, Bärtsch P, Mairbäurl H. Hypoxia causes permeability edema in the constant-pressure perfused rat lung. Eur Respir J 27: 600-606, 2006.
9. Egli M, Duplain H, Lepori M, Cook S, Nicod P, Hummler E, Sartori C, Scherrer U. Defective respiratory amiloride sensitive sodium transport predisposes to pulmonary oedema and delays its resolution in mice. J Physiol 560: 857-865, 2004.
10. Factor P, Saldias F, Ridge K, Dumasius V, Zabner J, Jaffe HA, Blanco G, Barnard M, Mercer R, Perrin R, Sznajder JI. Augmentation of lung liquid clearance via adenovirus- mediated transfer of a Na,K-ATPase beta(1) subunit gene. J Clin Invest 102: 1421-1430, 1998.
11. Folkesson HG, Norlin A, Wang YB, Abedinpour P, Matthay MA. Dexamethasone and thyroid hormone pretreatment upregulate alveolar epithelial fluid clearance in adult rats. J Appl Physiol 88: 416-424, 2000.
12. Heerlein K, Schulze A, Hotz L, Bärtsch P, Mairbäurl H. Hypoxia decreases cellular ATP-demand and inhibits mitochondrial respiration of A549 cells. Am J Respir Cell Mol Biol 32: 44-51, 2005.
13. Hochachka PW. Defense strategies against hypoxia and hypothermia. Science 231: 234-241, 1986.
14. Hummler E, Barker P, Gatzy J, Beermann F, Verdumo C, Boucher R, Rossier BC. Early death due to defective neonatal lung liquid clearance in α-ENaC-deficient mice. Nat Genet 13: 325-328, 1996.
15. + channels by dexamethasone. Am J Physiol Cell Physiol 279: C762-C770, 2000.
16. Maggiorini M, Brunner-La Rocca H, Peth S, Fischler M, Böhm T, Bernheim A, Kiencke S, Bloch KE, Dehnert C, Naeije R, Lehmann T, Bärtsch P, Mairbäurl H. Both tadalafil and dexamethasone may reduce the incidence of high-asltitude pulmonary edema. Ann Intern Med 145: 497-506, 2006.
17. Maggiorini M, Melot C, Pierre S, Pfeiffer F, Greve I, Sartori C, Lepori M, Hauser M, Scherrer U, Naeije R. High-altitude pulmonary edema is initially caused by an increase in capillary pressure. Circulation 103: 2078-2083, 2001.
18. Mairbäurl H. Role of alveolar epithelial sodium transport in high altitude pulmonary edema (HAPE). Respir Physiol Neurobiol 151: 178-191, 2006.
19. Mairbäurl H, Mayer K, Kim KJ, Borok Z, Bärtsch P, Crandall ED. Hypoxia decreases active Na transport across primary rat alveolar epithelial cell monolayers. Am J Physiol Lung Cell Mol Physiol 282: L659-L665, 2002.
20. Mairbäurl H, Weymann J, Möhrlein A, Swenson ER, Maggiorini M, Gibbs JS, Bärtsch P. Nasal epithelium potential difference at high altitude (4559 m): evidence for secretion. Am J Respir Crit Care Med 167: 862-867, 2003.
21. Mairbäurl H, Wodopia R, Eckes S, Schulz S, Bärtsch P. Impairment of cation transport in A549 cells and rat alveolar epithelial cells by hypoxia. Am J Physiol Lung Cell Mol Physiol 273: L797-L806, 1997.
22. Matalon S. Mechanisms and regulation of ion transport in adult mammalian alveolar type II pneumocytes. Am J Physiol Cell Physiol 261: C727-C738, 1991.
23. Matthay MA, Clerici C, Saumon G. Active fluid clearance from distal airspaces. J Appl Physiol 93: 1533-1541, 2002.
24. Matthay MA, Folkesson HG, Clerici C. Lung epithelial fluid transport and the resolution of pulmonary edema. Physiol Rev 82: 569-600, 2002.
25. Matthay MA, Folkesson HG, Verkman AS. Salt and water transport across alveolar and distal airway epithelia in the adult lung. Am J Physiol Lung Cell Mol Physiol 270: L487-L503, 1996.
26. Matthay MA, Wiener-Kronish JP. Intact epithelial barrier function is critical for the resolution of alveolar edema in humans. Am Rev Respir Dis 142: 1250-1257, 1990.
27. Noda M, Suzuki S, Tsubochi H, Sugita M, Maeda S, Kobayashi S, Kubo H, Kondo T. Single dexamethasone injection increases alveolar fluid clearance in adult rats. Crit Care Med 31: 1183-1189, 2003.
28. Planes C, Blot-Chabaud M, Matthay MA, Couette S, Uchida T, Clerici C. Hypoxia and beta(2)-agonists regulate cell surface expression of the epithelial sodium channel in native alveolar epithelial cells. J Biol Chem 277: 47318-47324, 2002.
29. Planes C, Escoubet B, Blot-Chabaud M, Friedlander G, Farman N, Clerici C. Hypoxia downregulates expression and activity of epithelial sodium channels in rat alveolar epithelial cells. Am J Respir Cell Mol Biol 17: 508-518, 1997.
30. Planes C, Friedlander G, Loiseau A, Amiel C, Clerici C. Inhibition of Na-K-ATPase activity after prolonged hypoxia in an alveolar epithelial cell line. Am J Physiol Lung Cell Mol Physiol 271: L70-L78, 1996.
31. Richalet JP. High altitude pulmonary oedema: still a place for controversy? Thorax 50: 923-929, 1995.
32. Sartori C, Allemann Y, Duplain H, Lepori M, Egli M, Lipp E, Hutter D, Turini P, Hugli O, Cook S, Nicod P, Scherrer U. Salmeterol for the prevention of high-altitude pulmonary edema. N Engl J Med 346: 1631-1636, 2002.
33. +-ATPase pump in a mouse model in vivo augments resolution of high permeability pulmonary oedema. Gene Ther 7: 960-966, 2000.
34. Vivona ML, Matthay MA, Chabaud MB, Friedlander G, Clerici C. Hypoxia reduces alveolar epithelial sodium and fluid transport in rats: reversal by beta-adrenergic agonist treatment. Am J Respir Cell Mol Biol 25: 554-561, 2001.
35. Ware LB, Matthay MA. Alveolar fluid clearance is impaired in the majority of patients with acute lung injury and the acute respiratory distress syndrome. Am J Respir Crit Care Med 163: 1376-1383, 2001.
36. Wodopia R, Ko HS, Billian J, Wiesner R, Bärtsch P, Mairbäurl H. Hypoxia decreases proteins involved in transepithelial electrolyte transport of A549 cells and rat lung. Am J Physiol Lung Cell Mol Physiol 279: L1110-L1119, 2000.