CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 157, Issue 1, 2008, Pages 73-82

  Scahill, Michael D ^a   Pastar, Irena ^a   Cross, George A M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

CRE recombinase; Culture; Fluoroorotic acid; Ganciclovir; Genetic tools; Negative selection; Pyrimidine auxotroph; Pyrimidine synthesis; Thymidine; Trypanosoma brucei; ura3

Indexed keywords

CRE RECOMBINASE; FLUOROOROTIC ACID; GANCICLOVIR; GENOMIC DNA; PYRIMIDINE; TETRACYCLINE; THYMIDINE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; GENE CONVERSION; GENETIC MANIPULATION; GENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

ANIMALS; GENE DELETION; GENETICS, MICROBIAL; INTEGRASES; MUTAGENESIS, INSERTIONAL; PARASITOLOGY; RECOMBINATION, GENETIC; SELECTION (GENETICS); TRYPANOSOMA BRUCEI BRUCEI;

TRYPANOSOMA BRUCEI;

EID: 36348958426     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2007.10.003     Document Type: Article

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