Molecular cloning and electrophysiological studies on the first K+ channel toxin (LmKTx8) derived from scorpion Lychas mucronatus

Peptides

Volumn 28, Issue 12, 2007, Pages 2306-2312

  Wu, Wenlan ^a   Yin, Shijin ^a,b   Ma, Yibao ^a   Wu, Ying Liang ^a   Zhao, Ruiming ^a   Gan, Geliang ^c   Ding, Jiuping ^c   Cao, Zhijian ^a   Li, Wenxin ^a  

^a WUHAN UNIVERSITY   (China)

^b SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES   (China)

^c HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

KTx; BK; Kv1.3; LmKTx8; Lychas mucronatus

Indexed keywords

PROTEIN LMKTX8; TOXIN; UNCLASSIFIED DRUG; VOLTAGE GATED POTASSIUM CHANNEL;

AMINO ACID SEQUENCE; ARTICLE; ELECTROPHYSIOLOGY; GENE EXPRESSION; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SCORPION; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; ELECTROPHYSIOLOGY; HUMANS; KV1.3 POTASSIUM CHANNEL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNEL BLOCKERS; RECOMBINANT FUSION PROTEINS; SCORPION VENOMS; SCORPIONS; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

LYCHAS MUCRONATUS; SCORPIONES;

EID: 36149000551     PISSN: 01969781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.peptides.2007.10.009     Document Type: Article

References (36)

1. Abdel-Mottaleb Y., Coronas F.V., de Roodt A.R., Possani L.D., and Tytgat J. A novel toxin from the venom of the scorpion Tityus trivittatus, is the first member of a new alpha-KTX subfamily. FEBS Lett 580 (2006) 592-596
2. Bagdany M., Batista C.V., Valdez-Cruz N.A., Somodi S., Rodriguez de la Vega R.C., Licea A.F., et al. Anuroctoxin, a new scorpion toxin of the alpha-KTx 6 subfamily, is highly selective for Kv1.3 over IKCa1 ion channels of human T lymphocytes. Mol Pharmacol 67 (2005) 1034-1044
3. Batista C.V., Roman-Gonzalez S.A., Salas-Castillo S.P., Zamudio F.Z., Gomez-Lagunas F., and Possani L.D. Proteomic analysis of the venom from the scorpion Tityus stigmurus: biochemical and physiological comparison with other Tityus species. Comp Biochem Physiol C Toxicol Pharmacol 146 (2007) 147-157
4. Bendtsen J.D., Nielsen H., von Heijne G., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340 (2004) 783-795
5. Coetzee W.A., Amarillo Y., Chiu J., Chow A., Lau D., McCormack T., et al. Molecular diversity of K+ channels. Ann NY Acad Sci 868 (1999) 233-285
6. Curran M.E. Potassium ion channels and human disease: phenotypes to drug targets?. Curr Opin Biotechnol 9 (1998) 565-572
7. Ferrat G., Bernard C., Fremont V., Mullmann T.J., Giangiacomo K.M., and Darbon H. Structural basis for alpha-K toxin specificity for K+ channels revealed through the solution 1H NMR structures of two noxiustoxin-iberiotoxin chimeras. Biochemistry 40 (2001) 10998-11006
8. + channels. Toxicon 36 (1998) 1641-1650
9. Garcia M.L., Hanner M., Knaus H.G., Slaughter R., and Kaczorowski G.J. Scorpion toxins as tools for studying potassium channels. Methods Enzymol 294 (1999) 624-639
10. Luo F., Zeng X.C., Hahin R., Cao Z.J., Liu H., and Li W.X. Genomic organization of four novel nondisulfide-bridged peptides from scorpion Mesobuthus martensii Karsch: gaining insight into evolutionary mechanism. Peptides 26 (2005) 2427-2433
11. Mao X., Cao Z., Yin S., Ma Y., Wu Y., and Li W. Cloning and characterization of BmK86, a novel K(+)-channel blocker from scorpion venom. Biochem Biophys Res Commun (2007)
12. Mihalek I., Res I., and Lichtarge O. A family of evolution-entropy hybrid methods for ranking protein residues by importance. J Mol Biol 336 (2004) 1265-1282
13. Mouhat S., Jouirou B., Mosbah A., De Waard M., and Sabatier J.M. Diversity of folds in animal toxins acting on ion channels. Biochem J 378 (2004) 717-726
14. + channel types targeted by synthetic OSK1, a toxin from Orthochirus scrobiculosus scorpion venom. Biochem J 385 (2005) 95-104
15. Nielsen H., and Krogh A. Prediction of signal peptides and signal anchors by a hidden Markov model. Proc Int Conf Intell Syst Mol Biol 6 (1998) 122-130
16. Peng F., Zeng X.C., He X.H., Pu J., Li W.X., Zhu Z.H., et al. Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin. Eur J Biochem 269 (2002) 4468-4475
17. Pimenta A.M., Stocklin R., Favreau P., Bougis P.E., and Martin-Eauclaire M.F. Moving pieces in a proteomic puzzle: mass fingerprinting of toxic fractions from the venom of Tityus serrulatus (Scorpiones, Buthidae). Rapid Commun Mass Spectrom 15 (2001) 1562-1572
18. Possani L.D., Merino E., Corona M., Bolivar F., and Becerril B. Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie 82 (2000) 861-868
19. Regaya I., Beeton C., Ferrat G., Andreotti N., Darbon H., De Waard M., et al. Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins. J Biol Chem 279 (2004) 55690-55696
20. + channels and scorpion toxins. Trends Pharmacol Sci 24 (2003) 222-227
21. +-channels. Toxicon 43 (2004) 865-875
22. Schreiber M., and Salkoff L. A novel calcium-sensing domain in the BK channel. Biophys J 73 (1997) 1355-1363
23. Schwartz E.F., Diego-Garcia E., Rodriguez de la Vega R.C., and Possani L.D. Transcriptome analysis of the venom gland of the Mexican scorpion Hadrurus gertschi (Arachnida: Scorpiones). BMC Genomics 8 (2007) 119
24. Tan P.T., Srinivasan K.N., Seah S.H., Koh J.L., Tan T.W., Ranganathan S., et al. Accurate prediction of scorpion toxin functional properties from primary structures. J Mol Graph Model 24 (2005) 17-24
25. Tenenholz T.C., Klenk K.C., Matteson D.R., Blaustein M.P., and Weber D.J. Structural determinants of scorpion toxin affinity: the charybdotoxin (alpha-KTX) family of K(+)-channel blocking peptides. Rev Physiol Biochem Pharmacol 140 (2000) 135-185
26. Tong X., Yao J., He F., Chen X., Zheng X., Xie C., et al. NMR solution structure of BmK-betaIT, an excitatory scorpion beta-toxin without a 'hot spot' at the relevant position. Biochem Biophys Res Commun 349 (2006) 890-899
27. Tytgat J., Chandy K.G., Garcia M.L., Gutman G.A., Martin-Eauclaire M.F., van der Walt J.J., et al. A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies. Trends Pharmacol Sci 20 (1999) 444-447
28. Vianna-Jorge R., and Suarez-Kurtz G. Potassium channels in T lymphocytes: therapeutic targets for autoimmune disorders?. BioDrugs 18 (2004) 329-341
29. Wulff H., Calabresi P.A., Allie R., Yun S., Pennington M., Beeton C., et al. The voltage-gated Kv1.3 K(+) channel in effector memory T cells as new target for MS. J Clin Invest 111 (2003) 1703-1713
30. Xu J., Wang P., Li Y., Li G., Kaczmarek L.K., Wu Y., et al. The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity. Proc Natl Acad Sci USA 101 (2004) 3112-3117
31. Yao J., Chen X., Li H., Zhou Y., Yao L., Wu G., et al. BmP09, a "long chain" scorpion peptide blocker of BK channels. J Biol Chem 280 (2005) 14819-14828
32. Zeng X.C., Luo F., and Li W.X. Characterization of a novel cDNA encoding a short venom peptide derived from venom gland of scorpion Buthus martensii Karsch: trans-splicing may play an important role in the diversification of scorpion venom peptides. Peptides 27 (2006) 675-681
33. Zeng X.C., Luo F., and Li W.X. Molecular dissection of venom from Chinese scorpion Mesobuthus martensii: identification and characterization of four novel disulfide-bridged venom peptides. Peptides 27 (2006) 1745-1754
34. Zeng X.C., Zhu Z.H., Li W.X., Zhu S.Y., Peng F., Mao X., et al. Molecular cloning and genomic organization of a K(+) channel toxin from the Chinese scorpion Buthus martensii Karsch. Toxicon 39 (2001) 407-410
35. Zhijian C., Yun X., Chao D., Shunyi Z., Shijin Y., Yingliang W., et al. Cloning and characterization of a novel calcium channel toxin-like gene BmCa1 from Chinese scorpion Mesobuthus martensii Karsch. Peptides 27 (2006) 1235-1240
36. Zhu S., Li W., Zeng X., Jiang D., Mao X., and Liu H. Molecular cloning and sequencing of two 'short chain' and two 'long chain' K(+) channel-blocking peptides from the Chinese scorpion Buthus martensii Karsch. FEBS Lett 457 (1999) 509-514