Use of a nonhomologous end joining deficient strain (Δku70) of the ergot fungus Claviceps purpurea for identification of a nonribosomal peptide synthetase gene involved in ergotamine biosynthesis

Fungal Genetics and Biology

Volumn 45, Issue 1, 2008, Pages 35-44

  Haarmann, Thomas ^a   Lorenz, Nicole ^a   Tudzynski, Paul ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Claviceps purpurea; Ergocryptine; Ergot alkaloids; Ergot fungus; Ergotamine; Gene targeting efficiency; Homologous recombination; ku70; Nonhomologous end joining (NHEJ); Nonribosomal peptide synthetase (NRPS)

Indexed keywords

DEXTRO LYSERGYL PEPTIDE SYNTHASE; ERGOCRYPTINE; ERGOTAMINE; PEPTIDE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CLAVICEPS PURPUREA; FUNGAL GENE; FUNGAL GENETICS; FUNGAL STRAIN; FUNGUS MUTANT; GENE CLUSTER; GENE DELETION; GENE IDENTIFICATION; LPSA1 GENE; NONHUMAN; NONRIBOSOMAL PEPTIDE SYNTHESIS; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEOMICS;

CLAVICEPS; DNA-BINDING PROTEINS; ERGOLINES; ERGOTAMINE; EVOLUTION; FUNGAL PROTEINS; GENE TARGETING; GENES, FUNGAL; MULTIGENE FAMILY; MUTATION; PEPTIDE SYNTHASES; PLANT DISEASES; RECOMBINATION, GENETIC; SECALE CEREALE; SPECIES SPECIFICITY;

CLAVICEPS PURPUREA;

EID: 36049021933     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2007.04.008     Document Type: Article

References (44)

1. Aravind L., and Koonin E.V. SAP - a putative DNA-binding motif involved in chromosomal organization. Trends in Biochemical Sciences 25 (2000) 112-114
2. Aravind L., and Koonin E.V. Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system. Genome Research 11 (2001) 1365-1374
3. Bailey S.M., Julian M., Chen D.J., Kurimasa A., Li G.C., Lehnert B.E., and Goodwin E.H. DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes. Proceedings of the National Academy of Science of the USA 96 (1999) 14899-14904
4. Boulton S.J., and Jackson S.P. Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Research 24 (1996) 4639-4684
5. Cabanas M.J., Vazquez D., and Modolell J. Dual interference of hygromycin B with ribosomal translocation and with aminoacyltRNA recognition. European Journal of Biochemistry 87 (1978) 21-27
6. Correia T., Grammel N., Ortel I., Keller U., and Tudzynski P. Molecular cloning and analysis of the ergopeptine assembly system in the ergot fungus Claviceps purpurea. Chemistry and Biology 10 (2003) 1281-1292
7. Daley J.M., Palmbosm P.L., Wu D., and Wilson T.E. Nonhomologous end joining in yeast. Annual Reviews of Genetics 39 (2005) 431-451
8. KU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryotic Cell 5 (2006) 207-211
9. Goins C.L., Gerik K.J., and Lodge J.K. Improvements to gene deletion in the fungal pathogen Cryptococcus neoformans: absence of Ku proteins increases homologous recombination, and co-transformation of independent DNA molecules allows rapid complementation of deletion phenotypes. Fungal Genetics and Biology 43 (2006) 531-544
10. Gritz L., and Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25 (1983) 179-188
11. Haarmann T., Machado C., Lübbe Y., Correia T., Schardl C.L., Panaccione D.G., and Tudzynski P. The ergot alkaloid gene cluster in Claviceps purpurea: extension of the cluster sequence and intra species evolution. Phytochemistry 66 (2005) 1312-1320
12. Haarmann T., Ortel I., Tudzynski P., and Keller U. Identification of the cytochrome P450 monooxygenase that bridges the clavine and ergoline alkaloid pathways. ChemBioChem 7 (2006) 645-652
13. Haupt I., and Thrum H. Bacterial resistance to streptothricins. Journal of Basic Microbiology 25 (1985) 335-339
14. Hentges P., van Driessche B., Tafforeau L., Vandenhaute J., and Carr A.M. Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast 22 (2005) 1013-1019
15. Hsu H.L., Yannone S.M., and Chen D.J. Defining interactions between DNA-PK and ligase IV/XRCC4. DNA Repair (Amst.) 1 (2002) 225-235
16. Iwabuchi K., Hashimoto M., Matsui T., Kurihara T., Shimizu H., Adachi N., Ishiai M., Yamamoto K., Tauchi H., Tarata M., Koyama H., and Date T. 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis. Genes to Cells 11 (2006) 935-948
17. Kooistra R.K., Hooykaas P.J.J., and Steensma H.Y. Efficient gene targeting in Kluyveromyces lactis. Yeast 21 (2004) 781-792
18. Krappmann S., Sasse C., and Braus G.H. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryotic Cell 5 (2006) 212-215
19. Krugel H., Fiedler G., Smith C., and Baumberg S. Sequence and transcriptional analysis of the nourseothricin acetyltransferase encoding gene nat1 from Streptomyces noursei. Gene 127 (1993) 127-131
20. Marchler-Bauer A., and Bryant S.H. CD-search: protein domain annotations on the fly. Nucleic Acids Research 32 (2004) W327-W331
21. Meek K., Gupta S., Ramsden D.A., and Lees-Miller S.P. The DNA-dependent protein kinase: the director at the end. Immunological Reviews 200 (2004) 132-141
22. Mey G., Held K., Scheffer J., Tenberge K.B., and Tudzynski P. CPMK2, a SLT2-homologous mitogen-activated protein (MAP) kinase, is essential for pathogenesis of Claviceps purpurea on rye: evidence for a second conserved pathogenesis-related MAP kinase cascade in phytopathogenic fungi. Molecular Microbiology 46 (2002) 305-318
23. Nayak T., Szewczyk E., Oakley C.E., Osmani A., Ukil L., Murray S.L., Hynes M.J., Osmani S.A., and Oakley B.R. A versatile and efficient gene-targeting system for Aspergillus nidulans. Genetics 172 (2006) 1557-1566
24. Ninomiya Y., Suzuki K., Ishii C., and Inoue H. Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proceedings of the National Academy of Science, USA 101 (2004) 12248-12253
25. Pöggeler S., and Kück U. Highly efficient generation of signal transduction knockout mutants using a fungal strain deficient in the mammalian ku70 ortholog. Gene 378 (2006) 1-10
26. Reiter H., Milewskiy M., and Kelley P. Mode of action of phleomycin on Bacillus subtilis. Journal of Bacteriology 111 (1972) 586-592
27. Riederer B., Han M., and Keller U. d-Lysergyl peptide synthetase from the ergot fungus Claviceps purpurea. Journal of Biological Chemistry 271 (1996) 27524-27530
28. Riha K., Watson J.M., Parkey J., and Shippen D.E. Telomere length deregulation and enhanced sensitivity to genotoxic stress in Arabidopsis mutants deficient in Ku70. The EMBO Journal 21 (2002) 2819-2826
29. Schardl C.L., Panaccione D.G., and Tudzynski P. Ergot alkaloids - biology and molecular biology. The Alkaloids. Chemistry and Biology 63 (2006) 45-86
30. Scheffer J., Chen C., Heidrich P., Dickman M.B., and Tudzynski P. A CDC42 homologue in C. purpurea is involved in vegetative differentiation and is essential for pathogenicity. Eukaryotic Cell 4 (2005) 1228-1238
31. Scheffer J., Ziv C., Yarden O., and Tudzynski P. The COT homologue CPCOT1 regulates polar growth and branching and is essential for pathogenicity in Claviceps purpurea. Fungal Genetics and Biology 42 (2005) 107-118
32. Smit R., and Tudzynski P. Efficient transformation of Claviceps purpurea using pyrimidine auxotrophic mutants cloning of the OMP decarboxylase gene. Molecular and General Genetics 234 (1992) 297-305
33. Spagnolo L., Rivera-Calzada A., Pearl L.H., and Llorca O. Three-dimensional structure of the human DNA-PKcs/Ku70/Ku80 complex assembled on DNA and its implications for DNA DSB repair. Molecular Cell 22 (2006) 511-519
34. Sweigard J., Chumley F.G., Caroll A.M., Farrall L., and Valent B. A series of vectors for fungal transformation. Fungal Genetics Newsletter 44 (1997) 52-53
35. Takahashi T., Masuda T., and Koyama Y. Identification and analysis of ku70 and ku80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae. Bioscience, Biotechnology, and Biochemistry 70 (2006) 135-143
36. Tenberge K.B., Homann V., Oeser B., and Tudzynski P. Structure and expression of two polygalacturonase genes of Claviceps purpurea oriented in tandem and cytological evidence for pectinolytic enzyme activity during infection of rye. Phytopathology 86 (1996) 1084-1097
37. Tsai H.-F., Wang H., Gebler J.C., Poulter C.D., and Schardl C.L. The Claviceps purpurea gene encoding dimethylallyltryptophan synthase, the committed step for ergot alkaloid biosynthesis. Biochemical and Biophysical Research Communications 216 (1995) 119-125
38. Tudzynski P., Hölter K., Correia T., Arntz C., Grammel N., and Keller U. Evidence for an ergot alkaloid gene cluster in Claviceps purpurea. Molecular and General Genetic 261 (1999) 133-141
39. Tudzynski P., Correia T., and Keller U. Biotechnology and genetics of ergot alkaloids. Applied Microbiology and Biotechnology 57 (2001) 593-605
40. Tudzynski P., and Scheffer J. Claviceps purpurea: molecular aspects of a unique pathogenic lifestyle. Molecular Plant Pathology 5 (2004) 377-388
41. van Dyck E., Stasiak A.Z., Stasiak A., and West S.C. Binding of double-strand breaks in DNA by human Rad52 protein. Nature 398 (1999) 728-731
42. Vogel H.J. Distribution of lysine pathways among fungi: Evolutionary implications. American Naturalist 98 (1964) 435-446
43. Walker J.R., Corpina R.A., and Goldberg J. Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature 412 (2001) 607-614
44. Whittaker C.A., and Hynes R.O. Distribution and evolution of von willebrand/integrin A domains: widely dispersed domains with roles in cell adhesion and elsewhere. Molecular Biology of the Cell 13 (2002) 3369-3387