Organization of reconstituted lipoprotein MexA onto supported lipid membrane

European Biophysics Journal

Volumn 36, Issue 8, 2007, Pages 1029-1037

  Trépout, Sylvain ^a   Taveau, Jean Christophe ^a   Mornet, Stéphane ^a,c   Benabdelhak, Houssain ^b   Ducruix, Arnaud ^b   Lambert, Olivier ^a  

^a INSTITUT EUROPÉEN DE CHIMIE ET BIOLOGIE   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

^c JOINT RESEARCH CENTRE   (Italy)

Author keywords

Cryo electron microscopy; Membrane protein; Membrane protein on solid support; Multidrug resistance; QCM D; SLB

Indexed keywords

BETA OCTYL GLUCOSIDE; GLUCOSIDE; LIPOPROTEIN; LIPOPROTEIN MEXA; LIPOSOME; PERIPLASMIC PROTEIN; SILICATE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CYTOPLASM; LIPID BILAYER; LIPID MEMBRANE; MOLECULAR MIMICRY; NONHUMAN; ORIENTATION; PROTEIN ANALYSIS; PROTEIN STRUCTURE; PSEUDOMONAS AERUGINOSA; SOLUBILIZATION;

BACTERIAL OUTER MEMBRANE PROTEINS; CRYOELECTRON MICROSCOPY; DETERGENTS; GLUCOSIDES; INDICATORS AND REAGENTS; LIPID BILAYERS; MEMBRANE TRANSPORT PROTEINS; PHOSPHATIDYLCHOLINES; PSEUDOMONAS AERUGINOSA;

PSEUDOMONAS AERUGINOSA;

EID: 35748944867     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-007-0208-5     Document Type: Article

References (38)

1. Akama H, Kanemaki M, Yoshimura M, Tsukihara T, Kashiwagi T, Yoneyama H, Narita S, Nakagawa A, Nakae T (2004a) Crystal structure of the drug discharge outer membrane protein, OprM, of Pseudomonas aeruginosa: dual modes of membrane anchoring and occluded cavity end. J Biol Chem 279:52816-52819
2. Akama H, Matsuura T, Kashiwagi S, Yoneyama H, Narita S, Tsukihara T, Nakagawa A, Nakae T (2004b) Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. J Biol Chem 279:25939-25942
3. Andersen C, (2003) Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria. Rev Physiol Biochem Pharmacol 147:122-165
4. Angrand M, Briolay A, Ronzon F, Roux B (1997) Detergent-mediated reconstitution of a glycosyl-phosphatidylinositolprotein into liposomes. Eur J Biochem 250:168-176
5. Ataka K, Giess F, Knoll W, Haber-Pohlmeier S, Richter B, Heberle J (2004) Oriented attachment and membrane reconstitution of His-tagged cytochrome c oxidase to a gold electrode: in situ monitoring by surface-enhanced infrared absorption spectroscopy. J Am Chem Soc 126:16199-16206
6. Bader B, Kuhn K, Owen DJ, Waldmann H, Wittinghofer A, Kuhlmann J (2000) Bioorganic synthesis of lipid-modified proteins for the study of signal transduction. Nature 403:223-226
7. Fralick JA (1996) Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J Bacteriol 178:5803-5805
8. Grogan MJ, Kaizuka Y, Conrad RM, Groves JT, Bertozzi CR (2005) Synthesis of lipidated green fluorescent protein and its incorporation in supported lipid bilayers. J Am Chem Soc 127:14383-14387
9. Guzman LM, Belin D, Carson MJ, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 177:4121-4130
10. Higgins MK, Bokma E, Koronakis E, Hughes C, Koronakis V (2004) Structure of the periplasmic component of a bacterial drug efflux pump. Proc Natl Acad Sci USA 101:9994-9999
11. Hetzer M, Heinz S, Grage S, Bayerl TM (1998) Asymmetric molecular friction in supported phospholipid bilayers revealed by NMR measurements of lipid diffusion. Langmuir 14:982-984
12. Keller CA, Kasemo K (1998) Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance. Biophys J 75:1397-1402
13. Keller M, Kerth A, Blume A (1997) Thermodynamics of interaction of octyl glucoside with phosphatidylcholine vesicles: partitioning and solubilization as studied by high sensitivity titration calorimetry. Biochim Biophys Acta 1326:178-192
14. Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C (2000) Crystal structure of the bacterial embrane protein TolC central to multidrug efflux and protein export. Nature 405:914-919
15. Lambert O, Benabdelhak H, Chami M, Jouan L, Nouaille E, Ducruix A, Brisson A (2005) Trimeric structure of OprN and OprM efflux proteins from Pseudomonas aeruginosa, by 2D electron crystallography. J Struct Biol 150:50-57
16. Lehto MT, Sharom FJ (1998) Release of the glycosylphosphatidylinositol- anchored enzyme ecto-5'-nucleotidase by phospholipase C: catalytic activation and modulation by the lipid bilayer. Biochem J 332:101-109
17. leMaire M, Moller JV, Champeil P (1987) Binding of a non-ionic detergent to membranes: flip-flop rate and location on the bilayer. Biochemistry 26:4803-4810
18. Mikolosko J, Bobyk K, Zgurskaya HI, Ghosh P (2006) Conformational flexibility in the multidrug efflux system protein AcrA. Structure 14:577-587
19. Mornet S, Lambert O, Duguet E, Brisson A (2005) The formation of supported lipid bilayers on silica nanoparticles revealed by Cryoelectron microscopy. Nano Lett 5:281-285
20. Murakami S, Nakashima R, Yamashita E, Yamaguchi A (2002) Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419:587-593
21. Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A (2006) Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature 443:173-179
22. Nosjean S, Roux B (2003) Anchoring of glycosylphosphatidylinosiyol- proteins to liposomes. Methods Enzymol 12:216-232
23. Paternostre MT, Roux M, Rigaud JL (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by triton X-100, octyl glucoside, and sodium cholate. Biochemistry 27:2668-2677
24. Rasband WS 1997-2005 ImageJ U. S. National Institutes of Health, Bethesda, http://www.rsb.info.nih.gov/ij/
25. Reid-Taylor KL, Chu JWK, Sharom FJ (1999) Reconstitution of the glycosylphosphatidylinositol-anchored protein Thy-1: interaction with membrane phospholipids and galactosylceramide. Biochem Cell Biol 77:189-200
26. Richter R, Brisson A (2004) QCM-D on mica for parallel QCM-D-AFM studies. Langmuir 20:4609-4613
27. Richter R, Mukhopadhay A, Brisson A (2003) Pathways of lipid vesicle deposition on solid surfaces: a combined QCM-D and AFM study. Biophys J 85:3035-3047
28. Rigaud JL, Pitard B, Levy D (1995) Reconstitution of membrane proteins into liposomes: application to energy-transducing membrane proteins. Biochim Biophys Acta 1231:223-246
29. Rigaud JL, Mosser G, Lacapere JJ, Olofsson A, Levy D, Ranck JL (1997) Bio-Beads: an efficient strategy for two-dimensional crystallization of membrane proteins. J Struct Biol 118:226-235
30. Ronzon F, Morandat S, Roux B, Bortolato M (2004) Insertion of a glycosylphosphatidylinosiyol -anchored enzyme into liposomes. J Membrane Biol 197:167-177
31. Salafsky J, Groves JT, Boxer SG (1996) Architecture and function of membrane proteins in planar supported bilayers: a study with photosynthetic reaction centers. Biochemistry 35:14773-14781
32. Sennhauser G, Amstutz P, Briand C, Storchenegger O, Gruetter MG (2007) Drug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. PLoS Biol 5:e7
33. Sharom FJ, Lehto MT (2002) Glycosylphosphatidylinositol-anchored proteins: structure, function, and cleavage by phosphatidylinositol-specific phospholipase C. Biochem Cell Biol 80:535-549
34. Silvius JR (1992) Solubilization and Functional Reconstitution of Biomembrane Components. Annu Rev Biophys Biomol Struct 21:323-348
35. Tamura N, Murakami S, Oyama Y, Ishiguro M, Yamaguchi A (2005) Direct interaction of multidrug efflux transporter AcrB and outer membrane channel TolC detected via site-directed disulfide crosslinking. Biochemistry 44:11115-11121
36. Trépout S, Mornet S, Benabdelhak H, Ducruix A, Brisson AR, Lambert O (2007) Membrane protein selectively oriented on solid support and reconstituted into a lipid membrane. Langmuir 23:2647-2654
37. Wenk MR, Alt T, Seelig A, Seelig J (1997) Octyl-β-Dglucopyranoside partitioning into lipid bilayers: thermodynamics of binding and structural changes of the bilayer. Biophys J 72:1719-1731
38. Yoneyama H, Maseda H, Kamiguchi H, Nakae T (2000) Function of the membrane fusion protein, MexA, of the MexAB-OprM efflux pump in Pseudomonas aeruginosa without an anchoring membrane. J Biol Chem 275:4628-4634