Insertion of a glycosylphosphatidylinositol-anchored enzyme into liposomes

Journal of Membrane Biology

Volumn 197, Issue 3, 2004, Pages 169-177

  Ronzon, F ^a   Morandat, S ^a   Roux, B ^a   Bortolato, M ^a  

^a UNIVERSITÉ LYON 1   (France)

Author keywords

Alkaline phosphatase; Electron cryomicroscopy; Lipidic anchors; Liposomes; Protein organization into liposomes; Reconstitution

Indexed keywords

ALKALINE PHOSPHATASE; DETERGENT; GLYCOSYLPHOSPHATIDYLINOSITOL; HYDROGEN; LIPOSOME; RESIN;

ARTICLE; ELECTRON MICROSCOPY; ENZYME ACTIVITY; FLOTATION; GENE INSERTION; HYDROPHOBICITY; ISOTOPE LABELING; LIPID BILAYER; PROTEIN LIPID INTERACTION; RADIOACTIVITY; SUCROSE GRADIENT;

ALKALINE PHOSPHATASE; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLYCOSYLPHOSPHATIDYLINOSITOLS; KINETICS; LIPID BILAYERS; LIPOSOMES; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MOLECULAR CONFORMATION; PROTEIN BINDING; PROTEIN CONFORMATION;

INSERTION SEQUENCES;

EID: 1642333160     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-004-0651-5     Document Type: Article

References (42)

1. Angrand, M., Briolay, A., Ronzon, F., Roux, B. 1997. Detergent-mediated reconstitution of a glycosyl-phosphatidylinositol-protein into liposomes. Eur. J. Biochem. 250:168-176
2. Armesto, J., Hannappel, E., Leopold, K., Fischer, W., Bublitz, R., Langer, L., Cumme, G.A., Horn, A. 1996. Microheterogeneity of the hydrophobic and hydrophilic part of the glycosylphosphatidylinositol anchor of alkaline phosphatase from calf intestine. Eur. J. Biochem. 238:259-269
3. Benting, J., Rietveld, A., Ansorge, I., Simons, K. 1999b. Acyl and alkyl chain length of GPI-anchors is critical for raft association in vitro. FEES Lett. 462:47-50
4. Benting, J.H., Rietveld, A.G., Simons, K. 1999a. N-Glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells. J. Cell Biol. 146:313-320
5. Blume, A. 1979. A comparative study of the phase transitions of phospholipid bilayers and monolayers. Biochim. Biophys. Acta 557:32-44
6. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254
7. Brown, D.A., London, E. 1998. Functions of lipid rafts in biological membranes. Annu. Rev. Cell Dev. Biol. 14:111-136
8. Bublitz, R., Armesto, J., Hoffmann-Blume, E., Schulze, M., Rhode, H., Horn, A., Aulwurm, S., Hannappel, E., Fischer, W. 1993. Heterogeneity of glycosylphosphatidylinositol-anchored alkaline phosphatase of calf intestine. Eur. J. Biochem. 217:199-207
9. Cinek, T., Horejsi, V. 1992. The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J. Immunol. 149:2262-2270
10. Coleman, J.E., Gettins, P. 1983. Alkaline phosphatase, solution structure, and mechanism. Adv. Enzymol. Relat. Areas. Mol. Biol. 55:381-452
11. Cyboron, G.W., Wuthier, R.E. 1981. Purification and initial characterization of intrinsic membrane-bound alkaline phosphatase from chicken epiphyseal cartilage. J. Biol. Chem. 256:7262-7268
12. Dubochet, J., Adrian, M., Chang, J.J., Homo, J.C., Lepault, J., McDowall, A.W., Schultz, P. 1988. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21:129-228
13. Ferguson, M.A. 1999. The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research. J. Cell Sci. 112:2799-2809
14. Friedrichson, T., Kurzchalia, T.V. 1998. Microdomains of GPI-anchored proteins in living cells revealed by crosslinking. Nature 394:802-805
15. Harder, T., Simons, K. 1997. Caveolae, D1Gs, and the dynamics of sphingolipid-cholesterol microdomains. Curr. Opin. Cell Biol. 9:534-542
16. Holloway, P.W. 1973. A simple procedure for removal of Triton X-100 from protein samples. Anal. Biochem. 53:304-308
17. Hooper, N.M. 1992. More than just a membrane anchor. Curr. Biol. 2:617-619
18. Hooper, N.M. 1999. Detergent-insoluble glycosphingolipid/ cholesterol-rich membrane domains, lipid rafts and caveolae. Mol. Membr. Biol. 16:145-156
19. Huang, C., Mason, J.T. 1978. Geometric packing constraints in egg phosphatidylcholine vesicles. Proc. Natl. Acad. Sci. USA 75: 308-310
20. Jacobson, K., Sheets, E.D., Simson, R. 1995. Revisiting the fluid mosaic model of membranes. Science 268:1441-1442
21. Le Du, M.H., Stigbrand, T., Taussig, M.J., Menez, A., Stura, E.A. 2001. Crystal structure of alkaline phosphatase from human placenta at 1.8 A resolution. Implication for a substrate specificity. J. Biol. Chem. 276:9158-9165
22. Lehto, M.T., Sharom, F.J. 2002. Proximity of the protein moiety of a GPI-anchored protein to the membrane surface: a FRET study. Biochemistry 41:8368-8376
23. Low, M.G., Zilversmit, D.B. 1980. Role of phosphatidylinositol in attachment of alkaline phosphatase to membranes. Biochemistry 19:3913-3918
24. Madore, N., Smith, K.L., Graham, C.H., Jen, A., Brady, K., Hall, S., Morris, R. 1999. Functionally different GPI proteins are organized in different domains on the neuronal surface. EMBO J. 18:6917-6926
25. Marsh, D. 1996. Lateral pressure in membranes. Biochim. Biophys. Acta 1286:183-223
26. Milhiet, P.E., Giocondi, M.C., Baghdadi, O., Ronzon, F., Roux, B., Le Grimellec, C. 2002. Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts. EMBO Rep. 3:485-490
27. Morandat, S., Bortolato, M., Roux, B. 2002. Cholesterol-dependent insertion of glycosylphosphatidylinositol-anchored enzyme. Biochim. Biophys. Acta-Biomembranes 1564:473-478
28. Mouritsen, O.G., Jørgensen, K. 1997. Small-scale lipid-membrane structure: simulation versus experiment. Curr. Opin. Struct. Biol. 7:518-527
29. Nosjean, O., Briolay, A., Roux, B. 1997. Mammalian GPI proteins: sorting, membrane residence and functions. Biochim. Biophys. Acta 1331:153-186
30. Nosjean, O., Roux, B. 1999. Ectoplasmic insertion of a glycosylphosphatidylinositol-anchored protein in glycosphingolipid-and cholesterol-containing phosphatidylcholine vesicles. Eur. J. Biochem. 263:865-870
31. Read, S.M., Northcote, D.H. 1981. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal. Biochem. 116:53-64
32. Rietveld, A., Simons, K. 1998. The differential miscibility of lipids as the basis for the formation of functional membrane rafts. Biochim. Biophys. Acta 1376:467-479
33. Rigaud, J.L., Pitard, B., Levy, D. 1995. Reconstitution of membrane proteins into liposomes: application to energy-transducing membrane proteins. Biochim. Biophys. Acta 1231:223-246
34. Ronzon, F., Desbat, B., Buffeteau, T., Mingotaud, C., Chauvet, J.P., Roux, B. 2002a. Structure and orientation of a glycosylphosphatidyl inositol anchored protein at the air/water/interface. J. Phys. Chem. B 106:3307-3315
35. Ronzon, F., Desbat, B., Chauvet, J.P., Roux, B. 2002b. Behavior of a GPI-anchored protein in phospholipid monolayers at the air-water interface. Biochim. Biophys. Acta 1560:1-13
36. Ronzon, F., Desbat, B., Chauvet, J.-P., Roux, B. 2002c. Penetration of a GPI-anchored protein into phospholipid monolayers spread at the air/water interface. Colloids and Surfaces B: Biointerfaces 23:365-373
37. Rothberg, K.G. 1995. Caveolar targeting of glycosylphosphatidylinositol- anchored proteins. Methods Enzymol. 250:669-679
38. Schreier, H., Moran, P., Caras, I.W. 1994. Targeting of liposomes to cells expressing CD4 using glycosylphosphatidylinositol-anchored gp120. Influence of liposome composition on intracellular trafficking. J. Biol. Chem. 269:9090-9098
39. Schroeder, R.J., Ahmed, S.N., Zhu, Y., London, E., Brown, D.A. 1998. Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositol-anchored proteins by promoting the formation of detergent-insoluble ordered membrane domains. J. Biol. Chem. 273:1150-1157
40. Seelig, A. 1987. Local anesthetics and pressure: a comparison of dibucaine binding to lipid monolayers and bilayers. Biochim. Biophys. Acta 899:196-204
41. Simons, K., Ikonen, E. 1997. Functional rafts in cell membranes. Nature 387:569-572
42. Varma, R., Mayor, S. 1998. GPI-anchored proteins are organized in submicron domains at the cell surface. Nature 394:798-801