Bioorganic synthesis of lipid-modified proteins for the study of signal transduction

Nature

Volumn 403, Issue 6766, 2000, Pages 223-226

  Bader, Benjamin ^a   Kuhn, Karsten ^a,b   Owen, David J ^a,b,c   Waldmann, Herbert ^a,b   Wittinghofer, Alfred ^a   Kuhlmann, Jürgen ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

^c MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; LIPOPEPTIDE; LIPOPROTEIN; RAS PROTEIN;

ANIMAL CELL; ARTICLE; CELL TRANSFORMATION; GENE EXPRESSION; GENE MUTATION; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; RAT; SIGNAL TRANSDUCTION;

ANIMALS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; LIPID METABOLISM; LIPOPROTEINS; MEMBRANES, ARTIFICIAL; PC12 CELLS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RAS PROTEINS; RATS; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION;

ANIMALIA; BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 0034642495     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35003249     Document Type: Article

References (21)

1. Resh, M. D. Regulation of cellular signalling by fatty acid acylation and prenylation of signal transduction proteins. Cell. Signal. 8, 403-412 (1996).
2. Wittinghofer, A. Signal transduction via Ras. Biol. Chem. 379, 933-973 (1998).
3. Gelb, M. H. Protein prcnylation, et cetera: signal transduction in two dimensions. Science 275, 1750-1751 (1997).
4. Waddick, K. G. & Uckun, F. M. Innovative treatment programs against cancer. 1. Ras oncoprotein as a molecular target. Biochem. Pharmacol. 56, 1431-1426 (1998).
5. Page, M. J. et al. Expression and characterization of the Ha-ras p21 protein produced at high levels in the insect/baculovirus system. J. Biol. Chem. 264, 19147-19154 (1989).
6. Schmitttberger, T., Cotté, A. & Waldmann, H. Synthesis of characteristic lipopeptides of lipid modified proteins employing the allylester as protecting group, Chem. Comm., 937-938 (1998).
7. Nagele, E., Schelhaas, M., Kuder, N. & Waldmann, H. Chemoenzymatic synthesis of N-Ras lipopeptides. J. Am. Chem. Soc. 120, 6889-6902 (1998).
8. Tucker, J. et al. Expression of p21 proteins in Escherichia coli and stereochemistry of the nucleotide-binding site. EMBO J. 5, 1351-1358 (1986).
9. Bordier, C. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 256, 1604-1607 (1981).
10. Kalb, E., Frey, S. & Tamm, L. K. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers. Biochim. Biophys. Acta 1103, 307-316 (1992).
11. Schelhaas, M. et al. Chemoenzymatic synthesis of biotinylated ras peptides and their use in membrane binding studies of lipidated model proteins by surface plasmon resonance. Chem. Eur. J. 5, 1239-1252 (1999).
12. Herrmann, C., Martin, G. A. & Wittinghofer, A. Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein kianse. Biol. Chem. 270, 2901-2905 (1995).
13. Lenzen, C., Cool, R. H., Prinz, H., Kuhlmann, J. & Wittinghofer, A. Kinetic analysis by fluorescence of the interaction between Ras and the catalytic domain of the guanine nucleotide exchange factor Cdc25Mm. Biochemistry 37, 7420-7930 (1998).
14. Bar-Sagi, D. & Feramisco, J. R. Microinjection of the ras oncogens protein into PC12 cells induces morphological differentiation. Cell 42, 841-848 (1985).
15. Schmidt, G. et al. Biochemical and biological consequences of changing the specificity of p21 ras from guanosine to xanthosine nucleotides. Oncogene 12, 87-96 (1996).
16. Willumsen, B. M., Cox, A. D., Solski, P. A., Der, C. J. & Buss, J. E. Novel determinants of H-Ras plasma membrane localization and transformation. Oncogene 13, 1901-1909 (1996).
17. Siddiqui, A. A., Garland, J. R., Dalton, M. B. & Sinensky, M. Evidence for a high affinity, saturable, prenylation-dependent p21 Ha-ras binding site in plasma membranes. J. Biol. Chem. 273, 3712-3717 (1998).
18. Shahinian, S. & Silvius, J. R. Doubly-lipid-modified protein sequence motifs exhibit long-lived anchorage to lipid bilayer membranes. Biochemistry 34, 3813-3822 (1995).
19. MacDonald, R. C. et al. Small-volume extrusion apparatus for preparation of large, unilamellar vesicles. Biochim. Biophys. Acta 1061, 297-303 (1991).
20. Mayer, L. D., Hope, M. J. & Cullis, P. R. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim. Biophys. Acta 858, 161-168 (1986).
21. Schelhaas, M., Glomsda, S., Hänsler, M., Jakubke, H. D. & Waldmann, H. Enzymatic synthesis of peptides and Ras lipopeptides employing choline ester as a solubilizing, protecting, and activating group. Angew. Chem. Int. Edn Engl. 35, 106-109 (1996).