Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

Journal of Biotechnology

Volumn 125, Issue 2, 2006, Pages 194-197

  Katzen, Federico ^a   Kudlicki, Wieslaw ^a  

^a INVITROGEN CORPORATION   (United States)

Author keywords

In vitro protein expression; N linked glycosylation; Signal sequence processing

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; SIGNAL PROCESSING;

IN VITRO PROTEIN EXPRESSION; N-LINKED GLYCOSYLATION; SIGNAL SEQUENCE PROCESSING;

BIOTECHNOLOGY;

CELL EXTRACT; SIGNAL PEPTIDE;

ARTICLE; CELL LYSATE; COST; GLYCOSYLATION; INSECT; LABORATORY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TIME;

ANIMALS; BIOTECHNOLOGY; CELL EXTRACTS; CELL LINE; CELL-FREE SYSTEM; GLYCOSYLATION; PROTEIN BIOSYNTHESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SORTING SIGNALS; REPRODUCIBILITY OF RESULTS; SPODOPTERA;

INSECTA;

EID: 33746904421     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.03.002     Document Type: Article

References (9)

1. Ezure, T., Higashide, S., Suzuki, T., Ito, M. and Endo, K., 2004. Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution. US Patent 2004/0191858.
2. Hartley J.L., Temple G.F., and Brasch M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 10 (2000) 1788-1795
3. Jackson A.M., Boutell J., Cooley N., and He M. Cell-free protein synthesis for proteomics. Brief Funct. Genomic Proteomic. 2 (2004) 308-319
4. Katzen F., Chang G., and Kudlicki W. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23 (2005) 150-156
5. Kronenberg H.M., Fennick B.J., and Vasicek T.J. Transport and cleavage of bacterial pre-beta-lactamase by mammalian microsomes. J. Cell Biol. 96 (1983) 1117-1119
6. Kubick S., Schacherl J., Fleischer-Notter H., Royall E., Roberts L.O., and Stiege W. In vitro translation in an insect-based cell-free system. In: Swartz J.R. (Ed). Cell-free Protein Expression (2003), Springer, Berlin 209-217
7. Tarui H., Imanishi S., and Hara T. A novel cell-free translation/glycosylation system prepared from insect cells. J. Biosci. Bioeng. 90 (2000) 508-514
8. Tarui H., Murata M., Tani I., Imanishi S., Nishikawa S., and Hara T. Establishment and characterization of cell-free translation/glycosylation in insect cell (Spodoptera frugiperda 21) extract prepared with high pressure treatment. Appl. Microbiol. Biotechnol. 55 (2001) 446-453
9. Trombetta S.E., Ganan S.A., and Parodi A.J. The UDP-Glc:glycoprotein glucosyltransferase is a soluble protein of the endoplasmic reticulum. Glycobiology 1 (1991) 155-161