Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata

Journal of Biochemistry and Molecular Biology

Volumn 40, Issue 5, 2007, Pages 625-635

  Huang, Zhuoshi ^a   Jiang, Keji ^a   Pi, Yan ^a   Hou, Rong ^a   Liao, Zhihua ^a,c   Cao, Ying ^a   Han, Xu ^a   Wang, Qian ^a   Sun, Xiaofen ^a   Tang, Kexuan ^a,b  

^a FUDAN UNIVERSITY   (China)

^b SHANGHAI JIAO TONG UNIVERSITY   (China)

^c SOUTHWEST UNIVERSITY   (China)

Author keywords

RACE; RT PCR; Squalene synthase; Taxus cuspidata; TcSqS

Indexed keywords

TAXUS; TAXUS CUSPIDATA;

EID: 35348902567     PISSN: 12258687     EISSN: 02191024     Source Type: Journal    
DOI: 10.5483/bmbrep.2007.40.5.625     Document Type: Article

References (48)

1. Abe, I., Rohmer, M. and Prestwish, G. D. (1993) Enzymatic cyclization of squalene and oxidosqualene to sterols and triterpenes. Chem. Rev. 93, 2189-2206.
2. Akamine, S., Nakamori, K., Chechetka, S. A., Banba, M., Umehara, Y., Kouchi, H., Izui, K. and Hata, S. (2003) cDNA cloning, mRNA expression, and mutational analysis of the squalene synthase gene of Lotus japonicus. Biochim. Biophys. Acta 1626, 97-101.
3. Baloglu, E. and Kingston, D. G. I. (1999) The taxane diterpenoids. J. Nat. Prod. 62, 1448-1472.
4. Bloch, K. (1992) Sterol molecule: structure, biosynthesis, and function. Steroids 57, 378-383.
5. Cane, D. E., Shim, J. H., Xue, Q., Fitzsimons, B. C. and Hohn, T. M. (1995) Trichodiene synthase. Identification of active site residues by site-directed mutagenesis. Biochemistry 34, 2480-2488.
6. Chappell, J. (1995) The biochemistry and molecular biology of isoprenoid metabolism. Plant Physiol. 107, 1-6.
7. Connolly, J. D. and Hill, R. A. (2005) Triterpenoids. Nat. Prod. Rep. 22, 487-503.
8. Creelman, R. A. and Mullet, J. E. (1997) Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 355-381.
9. Devarenne, T. P., Shin, D. H., Back, K., Yin, S. and Chappell, J. (1998) Molecular characterization of tobacco squalene synthase and regulation in response to fungal elicitor. Arch. Biochem. Biophys. 349, 205-215.
10. Durner, J., Shah, J. and Kessig, D. F. (1997) Salicylic acid and disease resistance in plants. Trends Plant Sci. 2, 266-274.
11. Fulton, D. C., Tait, M. and Threlfall, D. R. (1995) Comparative study of the inhibition of rat and tobacco squalene synthase by squalestatins. Phytochemistry 38, 1137-1141.
12. Gu, P., Ishii, Y., Spencer, T. A. and Shechter, I. (1998) Function-structure studies and identification of three enzyme domains involved in the catalytic activity in rat hepatic squalene synthase. J. Biol. Chem. 273, 12515-12525.
13. Hanley, K. and Chappell, J. (1992) Solubilization, Partial Purification, and Immunodetection of Squalene Synthetase from Tobacco Cell Suspension Cultures. Plant Physiol. 98, 215-220.
14. Hanley, K. M., Nicolas, O., Donaldson, T. B., Smith-Monroy, C., Robinson, G. W. and Hellmann, G. M. (1996) Molecular cloning, in vitro expression and characterization of a plant squalene synthetase cDNA. Plant Mol. Biol. 30, 1139-1151.
15. Hata, S., Sanmiya, K., Kouchi, H., Matsuoka, M., Yamamoto, N. and Izui, K. (1997) cDNA cloning of squalene synthase genes from mono- and dicotyledonous plants, and expression of the gene in rice. Plant Cell Physiol. 38, 1409-1413.
16. Hayashi, H., Hirota, A., Hiraoka, N. and Ikeshiro, Y. (1999) Molecular cloning and characterization of two cDNAs for Glycyrrhiza glabra squalene synthase. Biol. Pharm. Bull. 22, 947-950.
17. Hofmann, K. and Stoffel, W. (1993) TMbase - A database of membrane spanning proteins segments. Biol. Chem. Hoppe-Seyler 374, 166.
18. Inoue, T., Osumi, T. and Hata, S. (1995) Molecular cloning and functional expression of a cDNA for mouse squalene synthase. Biochim. Biophys. Acta 1260, 49-54.
19. Jennings, S. M., Tsay, Y. H., Fisch, T. M. and Robinson, G. W. (1991) Molecular cloning and characterization of the yeast gene for squalene synthetase. Proc. Natl. Acad. Sci. USA 88, 6038-6042.
20. Jones, P. J. H., MacDougall, D. E., Ntanios, F. and Vanstone, C. A. (1997) Dietary phytosterols as cholesterol-lowering agents in humans. Can. J. Physiol. Pharmacol. 75, 217-227.
21. Kazok, M. (1987) An analysis of 5′-noncoding sequence from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15, 8125-8148.
22. Keller, R. K. (1996). Squalene synthase inhibition alters metabolism of nonsterols in rat liver. Biochim. Biophys. Acta 1303, 169-179.
23. Kohler, J. and Goldspiel, B. R. (1994) Evaluation of new drug Paclitaxel (Taxol). Pharmacotherapy 14, 3-34.
24. Kribii, R., Arro, M., Del Arco, A., Gonzalez, V., Balcells, L., Delourme, D., Ferrer, A., Karst, F. and Boronat, A. (1997) Cloning and characterization of the Arabidopsis thaliana SQS1 gene encoding squalene synthase-involvement of the C-terminal region of the enzyme in the channeling of squalene through the sterol pathway. Eur. J. Biochem. 24, 61- 69.
25. Lambert, C., Leonard, N., De Bolle, X. and Depiereux, E. (2002) ESyPred3D: Prediction of proteins 3D structures. Bioinformatics 18, 1250-1256.
26. Lee, J. H., Yoon, Y. H., Kim, H. Y., Shin, D. H., Kim, D. U., Lee, I. J. and Kim, K. U. (2002) Cloning and expression of squalene synthase cDNA from hot pepper (Capsicum annuum L.). Mol. Cells 13, 436-443.
27. Lee, M. H., Jeong, J. H., Seo, J. W., Shin, C. G., Kim, Y. S., In, J. G., Yang, D. C., Yi, J. S. and Choi, Y. E. (2004) Enhanced triterpene and phytosterol biosynthesis in Panax ginseng overexpressing squalene synthase gene. Plant Cell Physiol. 45, 976-984.
28. Lescot, M., Déhais, P., Thijs, G., Marchal, K., Moreau, Y., Van de Peer, Y., Rouzé, P. and Rombauts, S. (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 30, 325-327.
29. Liao, Z., Chen, M., Guo, L., Gong, Y., Tang, F., Sun, X. and Tang, K. (2004) Rapid isolation of high-quality total RNA from taxus and ginkgo. Prep. Biochem. Biotechnol. 34, 209-214.
30. LoGrasso, P. V., Soltis, D. A. and Boettcher, B. R. (1993) Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase. Arch. Biochem. Biophys. 307, 193-199.
31. Merkulov, S., van Assema, F., Springer, J., Fernandez Del Carmen, A. and Mooibroek, H. (2000) Cloning and characterization of the Yarrowia lipolytica squalene synthase (SQS1) gene and functional complementation of the Saccharomyces cerevisiae erg9 mutation. Yeast 16, 197-206.
32. Nakashima, T., Inoue, T., Oka, A., Nishino, T., Osumi, T. and Hata, S. (1995) Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase. Proc. Natl. Acad. Sci. USA 92, 2328-2332.
33. Okada, S., Devarenne, T. P. and Chappell, J. (2000) Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B. Arch. Biochem. Biophys. 373, 307-317.
34. Oksman-Caldentey, K. M. and Inze, D. (2004) Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites. Trends Plant Sci. 9, 433 -440.
35. Osbourn, A. E. (2003) Saponins in cereals. Phytochemistry 62, 1-4.
36. Pandit, J., Danley, D. E., Schulte, G. K., Mazzalupo, S., Pauly, T. A., Hayward, C. M., Hamanaka, E. S., Thompson, J. F. and Harwood, H. J., Jr. (2000) Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis. J. Biol. Chem. 275, 30610-30617.
37. Rechards, E. J. (1995) Preparation and analysis of DNA; in Short Protocol in Molecular Biology, Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (eds.), pp. 36-38, John Wiley and Sons, New York, USA.
38. Robinson, G. W., Tsay, Y. H., Kienzle, B. K., Smith-Monroy, C. A. and Bishop, R. W. (1993) Conservation between human and fungal squalene synthetases: similarities in structure, function, and regulation. Mol. Cell Biol. 13, 2706-2717.
39. Shechter, I., Klinger, E., Rucker, M. L., Engstrom, R. G., Spirito, J. A., Islam, M. A., Boettcher, B. R. and Weinstein, D. B. (1992) Solubilization, purification, and characterization of a truncated form of rat hepatic squalene synthetase. J. Biol. Chem. 267, 8628-8635.
40. Shimada, H., Kondo, K., Fraser, P. D., Miura, Y., Saito, T. and Misawa, N. (1998) Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway. Appl. Env. Microbiol. 64, 2676-2680.
41. Song, L. (2003) Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants. Anal. Biochem. 317, 180-185.
42. Song, L. and Poulter, C. D. (1994) Yeast farnesyl-diphosphate synthase: site-directed mutagenesis of residues in highly conserved prenyltransferase domains I and II. Proc. Natl. Acad. Sci. USA 91, 3044-3048.
43. Stamellos, K. D., Shackelford, J. E., Shechter, I., Jiang, G., Conrad, D., Keller, G. A. and Krisans, S. K. (1993) Subcellular localization of squalene synthase in rat hepatic cells. Biochemical and immunochemical evidence. J. Biol. Chem. 268, 12825-12836.
44. Thompson, J. F., Danley, D. E., Mazzalupo, S., Milos, P. M., Lira, M. E. and Harwood, H. J., Jr. (1998) Truncation of human squalene synthase yields active, crystallizable protein. Arch. Biochem. Biophys. 350, 283-290.
45. Wasternack, C. and Parthier, B. (1997) Jasmonate signaled plant gene expression. Trends Plant Sci. 2, 302-307.
46. Wendt, K. U. and Schulz, G. E. (1998) Isoprenoid biosynthesis: manifold chemistry catalyzed by similar enzymes. Structure 6, 127-133.
47. Wentzinger, L. F., Bach, T. J. and Hartmann, M. A. (2002) Inhibition of squalene synthase and squalene epoxidase in tobacco cells triggers an up-regulation of 3-hydroxy-3-methylglutaryl coenzyme a reductase. Plant Physiol. 130, 334-346.
48. Yoshioka, H., Yamada, N. and Doke, N. (1999) cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans. Plant Cell Physiol. 40, 993-998.