Cloning and expression of squalene synthase cDNA from hot pepper (Capsicum annuum L.)

Molecules and Cells

Volumn 13, Issue 3, 2002, Pages 436-443

  Lee, Jung Hoon ^a   Yoon, Yong Hwi ^a   Kim, Hak Yoon ^b   Shin, Dong Hyun ^a   Kim, Dal Ung ^a   Lee, In Jung ^a   Kim, Kil Ung ^a  

^a Kyungpook National University   (South Korea)

^b Keimyung University   (South Korea)

Author keywords

Hot pepper; Sesquiterpene Cyclase; Squalene Synthase; UV Irradiation

Indexed keywords

SESQUITERPENE; SQUALENE SYNTHASE; COMPLEMENTARY DNA; LYASE; TRICHODIENE SYNTHETASE;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CLONING; ENZYME ACTIVITY; NICOTIANA BENTHAMIANA; PEPPER; PLANT PRODUCT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SOUTHERN BLOTTING; TOBACCO; BIOSYNTHESIS; DNA SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RADIATION EXPOSURE; SEQUENCE ALIGNMENT; ULTRAVIOLET RADIATION;

ARABIDOPSIS; ARABIDOPSIS THALIANA; CAPSICUM ANNUUM; CAPSICUM FRUTESCENS; NICOTIANA BENTHAMIANA; NICOTIANA TABACUM;

BASE SEQUENCE; BLOTTING, SOUTHERN; CAPSICUM; CARBON-CARBON LYASES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; ULTRAVIOLET RAYS;

EID: 1842847404     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Back, K. and Chappell, J. (1995) Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases. J. Biol. Chem. 270, 7375-7381.
2. Back, K., He, S., Kim, K. U., and Shin, D. H. (1998) Cloning and bacterial expression of a sesquiterpene cyclase, a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum. Plant Cell Physiol. 39, 899-904.
3. Back, K., Nah, J., Lee, S. B., Song, J. H., Shin, D. H., and Kim, H. Y. (2000) Cloning of sesquiterpene cyclase and its functional expression by domain swapping strategy. Mol. Cells 10, 220-225.
4. Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. Cane, D. E., Shim, J. H., Xue, Q., Fitzsimons, B. C., and Hohn, T. M. (1995) Trichodiene synthase. Identification of active site residues by site-directed mutagenesis. Biochemistry 34, 2480-2488.
6. Chappell, J. (1995) The biochemistry and molecular biology of isoprenoid metabolism. Plant Physiol. 107, 1-6.
7. Chappell, J. and Nable, R. (1987) Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor. Plant Physiol. 85, 469-473.
8. Chappell, J., Von Lanken, C., Vögeli, U., and Bhatt, P. (1989) Sterol and sesquiterpenoid biosynthesis during a growth cycle of tobacco cell suspension cultures. Plant Cell Rep. 8, 48-52.
9. Devarenne, T. P., Shin, D. H., Back, K., Yin, S., and Chappell, J. (1998) Molecular characterization of tobacco squalene synthase and regulation in response to fungal elicitor. Arch. Biochem. Biophys. 349, 205-215.
10. Faust, J. R., Goldstein, J. L., and Brown, M. S. (1979) Squalene synthetase activity in human fibroblasts: regulation via the low density lipoprotein receptor. Proc. Natl. Acad. Sci. USA 76, 5018-5022.
11. Gabrila, K. R. and Hans, C. R. (1987) Squalene synthase. J. Biol. Chem. 262, 1505-1509.
12. Goldstein, J. L. and Brown, M. S. (1990) Regulation of the mevalonate pathway. Nature 343, 425-430.
13. Gu, P., Ishii, Y., Spencer, T. A., and Shechter, I. (1998) Function-structure studies and identification of three enzyme domain involved in the catalytic activity in rat hepatic squalene synthase. J. Biol. Chem. 273, 12515-12525.
14. Hanley, K. M., Vicolas, O., Donaldson, T. B., Smith-Monroy, C., Robinson, G. W., and Hellmann, G. M. (1996) Molecular cloning, in vitro expression and characterization of a plant squalene synthetase cDNA. Plant Mol. Biol. 30, 1139-1151.
15. Jennings, S. M., Tsay, Y. H., Fisch, T. M., and Robinson, G. W. (1991) Molecular cloning and characterization of the yeast gene for squalene synthetase. Proc. Natl. Acad. Sci. USA 88, 6038-6042.
16. Kribii, R., Arro, M., Del Aroco, A., Gonzalez, V., Balcells, L., Delourme, D., Ferrer, A., Karst, F., and Boronat, A. (1997) Cloning and characterization of the Arabidopsis thaliana SQSI gene encoding squalene synthase. Involvement of the C-terminal region of the enzyme in the channeling of squalene through the sterol pathway. Eur. J. Biochem. 249, 61-69.
17. LoGrasso, P. V., Soltis, D. A., and Boettcher, B. R. (1993) Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase. Arch. Biochem. Biophys. 307, 193-199.
18. McGarvey, D. J. and Croteau, R. (1995) Terpenoid metabolism. Plant Cell 7, 1015-1026.
19. McKenzie, T. L., Jiang, G., Straubhaar, J. R., Conrad, D. G., and Sheehter, I. (1992) Molecular cloning, expression, and characterization of the cDNA for the rat hepatic squalene synthase. J. Biol. Chem. 267, 21368-21374.
20. Nakashima, T., Inoue, T., Oka, A., Nishino, T., Osumi, T., and Hata, S. (1995) Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase. Proc. Natl. Acad. Sci. USA 92, 2328-2332.
21. Robinson, G. W., Tsay, Y. H., Kienzle, B. K., Smith-Montroy, C. A., and Bishop, R. W. (1993) Conservation between human and fungal squalene synthetases: similarities in structure, function, and regulation. Mol. Cell. Biol. 13, 2706-2717.
22. Sanger, F., Nicklen, S., and Conlson, A. R. (1977) DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
23. Scott, O. R. and Arnold, J. B. (1988) Extraction of DNA from plant tissues; in Plant Molecular Biology Manual, A6, pp. 1-11, Kluwer Academic Publishers.
24. Song, L. and Poulter, C. D. (1994) Yeast farnesyl-diphosphate synthase: site-directed mutagenesis of residues in highly conserved prenyltransferase domains I and II. Proc. Natl. Acad. Sci. USA 91, 3044-3048.
25. Vögeli, U. and Chappell, J. (1988) Induction of sesquiterpene cyclase and suppression of squalene synthetase activities in plant cell cultures treated with fungal elicitor. Plant Physiol. 88, 1291-1296.
26. Yin, S., Mei, L., Newman, J., Back, K., and Chappell, J. (1997) Regulation of sesquiterpene cyclase gene expression. Plant Physiol. 115, 437-451.