Immortalization and characterization of pleomorphic adenoma cells by transfection with the hTERT gene

International Journal of Oncology

Volumn 31, Issue 2, 2007, Pages 339-344

  Kitagawa, Masae ^a,b   Ogawa, Ikuko ^b   Shima, Kaori ^c   Hashimoto, Sadamitsu ^c   Kudo, Yasusei ^a   Miyauchi, Mutsumi ^a   Tahara, Hidetoshi ^a   Shimono, Masaki ^c   Takata, Takashi ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY HOSPITAL   (Japan)

^c TOKYO DENTAL COLLEGE   (Japan)

Author keywords

hTERT; Immortalization; Pleomorphic adenoma

Indexed keywords

TELOMERASE;

ADENOMA; AGED; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CYTOPLASM; FEMALE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; PAROTID GLAND TUMOR; PATHOLOGY; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADENOMA; AGED; CELL DIFFERENTIATION; CYTOPLASM; FEMALE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; PAROTID NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TELOMERASE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 35148859841     PISSN: 10196439     EISSN: 17912423     Source Type: Journal    
DOI: 10.3892/ijo.31.2.339     Document Type: Article

References (27)

1. Takeuchi J, Sobue M, Yoshida M, Esaki T and Katoh Y: Pleomorphic adenoma of the salivary gland with special reference to histochemical and electron microscopic studies and biochemical analysis of glycosaminoglycans in vitro. Cancer 36: 1771-1789, 1975.
2. Zhao M, Takata T, Kudo Y, et al: Biosynthesis of glycosaminoglycans and aggrecan by tumor cells in salivary pleomorphic adenoma: ultrastructural evidence. J Oral Pathol Med 28: 442-450, 1999.
3. Harley CB, Kim NW, Prowse KR, et al: Telomerase, cell immortality, and cancer. Cold Spring Harb Symp Quant Biol 59: 307-315, 1994.
4. Meyerson M, Counter CM, Eaton EN, et al: hEST2, the putative human telomerase catalytic subunit gene, is upregulated in tumor cells and during immortalization. Cell 90: 785-795, 1997.
5. Nakamura TM, Mori GB, Chapman KB, et al: Telomerase catalystic subunit homologs from fission yeast and human, Science 277: 955-959, 1997.
6. Nakayama J-I, Tahara H, Tahara E, et al: Telomerase activation by TRT in human normal fibroblasts and hepatocellular carcinomas. Nat Genet 18: 65-68, 1998.
7. Ulner GA, Hu J-F, Vu TH, Giudice LC and Hoffman AR: Telomerase activity in human development is regulated by human telomerase reverse transcription and by alternate splicing of hTERT transcripts. Cancer Res 58: 4168-4172, 1998.
8. Bodnar AG, Ouellette M, Frolkis M, et al: Extension of lifespan by introduction of telomerase into normal human cells. Science 279: 349-352, 1998.
9. Ramakrishnan S, Eppenberger U, Muller H, Shinkai Y and Narayanan R: Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res 58: 622-625, 1998. (Pubitemid 28099468)
10. Meyerson M: Role of telomerase in normal and cancer cells: J Clin Oncol 18: 2626-2634, 2000.
11. Ulaner GA, Hu-J-F, Vu TH, Oruganri H, Giuduce LC and Hoffman AR: Regulation of telomerase by alternate splicing of human telomerase reverse transcriptase (hTERT) in normal and neoplastic ovary, endometrium, and myometrium. Int J Cancer 85: 330-335, 2000.
12. Zheng PS, Iwasaka T, Yamasaki F, et al: Telomerase activity in gynecologic tumors. Gynecol Oncol 64: 171-175, 1997.
13. Kim NW, Piatyszek MA, Prowse KR, et al: Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011-2015, 1994.
14. Kas K, Voz ML, Roijer E, Astrom AK, Meyen E, Stenman G and Van de Ven WJ: Promoter swapping between the genes for a novel zinc finger protein and beta-catenin in pleomorphic adenomas with t(3;8)(p21;q12) translocations. Nat Genet 15: 170-174, 1997.
15. Kas K, Voz ML, Hensen K, Meyen E and Van de Ven WJ: Transcriptional activation capacity of the novel PLGA family of zinc finger proteins. J Biol Chem 273: 23026-23032, 1998. (Pubitemid 28417481)
16. Jat PS and Sharp PA: Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts. J Virol 59: 746-750, 1986. (Pubitemid 16011141)
17. Munger K and Howley PM: Human papillomavirus immortalization and transformation functions. Virus Res 89: 213-228, 2002. (Pubitemid 35351540)
18. Shay JW, Wright WE and Werbin H: Defining the molecular mechanisms of human cell immortalization. Biochim Biophys Acta 1072: 1-7, 1991.
19. Carney AS, Tahara H, Swartz CD, et al: Immortalization of human uterine leiomyoma and myometrial cell lines after induction of telomerase activity: molecular and phenotypic characteristics. Lab Invest 82: 719-728, 2002.
20. Yoo GH, Washington J, Oliver J, et al: The effects of exogenous p53 overexpression on HPV-immortalized and carcinogen transformed oral keratinocytes. Cancer 94: 159-166, 2002.
21. Counter CM, Hahn WC, Wei W, et al: Dissociation among in vitro telomerase maintenance, and cellular immortalization. Proc Natl Acad Sci USA 95: 14723-14728, 1998.
22. Halvorson TL, Leibowitz G and Lexine F: Telomerase activity is sufficient to allow transformed cells to escape from crisis. Mol Cell Biol 19: 1864-1870, 1999.
23. Colgin LM and Reddel RR: Telomere maintenance mechanisms and cellular immortalization. Curr Opin Genet Dev 9: 97-103, 1999.
24. Furuse M, Hirase T, Itoh M, Nagafuchi A, Yonemura S, Tsukita S and Tsukita S: Occludin: a novel integral membrane protein localizing at tight junctions. J Cell Biol 123: 1777-1788, 1993.
25. Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S and Tsukita S: Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol 141: 1539-1550, 1998. (Pubitemid 28309169)
26. Stevenson BR, Siliciano JD, Mooseker MS and Goodenough DA: Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J Cell Biol 103: 755-766, 1986.
27. Tsukita S and Furuse M: Occludin and claudins in tight-junction strands: leading or supporting players? Trends Cell Biol 9: 268-273, 1999.