Probing insulin's secondary structure after entrapment into alginate/chitosan nanoparticles

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 65, Issue 1, 2007, Pages 10-17

  Sarmento, B ^a,b   Ferreira, D C ^a   Jorgensen, L ^b   van de Weert, M ^b  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Alginate; Chitosan; Circular dichroism; FTIR; Insulin; Nanoparticles; Secondary structure

Indexed keywords

ALGINIC ACID; CHITOSAN; INSULIN ZINC SUSPENSION;

ALPHA HELIX; ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; DRUG CONFORMATION; DRUG RELEASE; DRUG STRUCTURE; GASTROINTESTINAL TRACT; GELATION; INFRARED SPECTROSCOPY; NANOPARTICLE; PARTICLE SIZE; PROTEIN SECONDARY STRUCTURE; SIMULATION; STANDARD; STRUCTURE ANALYSIS;

ADMINISTRATION, ORAL; ALGINATES; CHEMISTRY, PHARMACEUTICAL; CHITOSAN; CIRCULAR DICHROISM; DELAYED-ACTION PREPARATIONS; DRUG CARRIERS; DRUG COMPOUNDING; DRUG STABILITY; GLUCURONIC ACID; HEXURONIC ACIDS; HYDROGEN-ION CONCENTRATION; HYPOGLYCEMIC AGENTS; INSULIN; KINETICS; NANOPARTICLES; PARTICLE SIZE; PROTEIN STRUCTURE, SECONDARY; SOLUBILITY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TECHNOLOGY, PHARMACEUTICAL;

EID: 34948841373     PISSN: 09396411     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejpb.2006.09.005     Document Type: Article

References (25)

1. Cui F., Shi K., Zhang L., Tao A., and Kawashima Y. Biodegradable nanoparticles loaded with insulin-phospholipid complex for oral delivery: preparation, in vitro characterization and in vivo evaluation. J. Control. Release 114 (2006) 242-250
2. Ma Z., Lim T.M., and Lim L.-Y. Pharmacological activity of peroral chitosan-insulin nanoparticles in diabetic rats. Int. J. Pharm. 293 (2005) 271-280
3. Fernandez-Urrusuno R., Calvo P., Remunan-Lopez C., Vila-Jato J.L., and Alonso M.J. Enhancement of nasal absorption of insulin using chitosan nanoparticles. Pharm. Res. 16 (1999) 1576-1581
4. Pan Y., Li Y.J., Zhao H.Y., Zheng J.M., Xu H., Wei G., Hao J.S., and Cui F.D. Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo. Int. J. Pharm. 249 (2002) 139-147
5. Damge C., Vrancks H., Balschmidt P., and Couvreur P. Poly(alkyl cyanoacrylate) nanospheres for oral administration of insulin. J. Pharm. Sci. 86 (1997) 1407-1500
6. Carino G.P., Jacob J.S., and Mathiowitz E. Nanosphere based oral insulin delivery. J. Control. Release 65 (2000) 261-269
7. Ma X.Y., Pan G.M., Lu Z., Hu J.S., Bei J.Z., Jia J.H., and Wang S.G. Preliminary study of oral polylactide microcapsulated insulin in vitro and in vivo. Diabetes Obes. Metab. 2 (2000) 243-250
8. Jung T., Kamm W., Breitenbach A., Kaiserling E., Xiao J.X., and Kissel T. Biodegradable nanoparticles for oral delivery of peptides: is there a role for polymers to affect mucosal uptake?. Eur. J. Pharm. Biopharm. 50 (2000) 147-160
9. Sarmento B., Martins S., Ribeiro A., Veiga F., Neufeld R., and Ferreira D. Development and comparison of different nanoparticulate polyelectrolyte complexes as insulin carriers. Int. J. Pept. Res. Ther. 12 (2006) 131-138
10. Tatford O.C., Gomme P.T., and Bertolini J. Analytical techniques for the evaluation of liquid protein therapeutics. Biotechnol. Appl. Biochem. 40 (2004) 67-81
11. Nielsen L., Frokjaer S., Brange J., Uversky V.N., and Fink A.L. Probing the mechanism of insulin fibril formation with insulin mutants. Biochemistry 40 (2001) 8397-8409
12. Nielsen L., Frokjaer S., Carpenter J., and Brange J. Studies of the structure of insulin fibrils by fourier transform infrared (FTIR) spectroscopy and electron microscopy. J. Pharm. Sci. 90 (2001) 29-37
13. van de Weert M., Andersen M.B., and Frokjaer S. Complex coacervation of lysozyme and heparin: complex characterization and protein stability. Pharm. Res. 21 (2004) 2354-2359
14. van de Weert M., Hennink W.E., and Jiskoot W. Protein instability in poly(lactic-co-glycolic acid) microparticles. Pharm. Res. 17 (2000) 1159-1167
15. Tiyaboonchai W., Woiszwillo J., Sims R.C., and Middaugh C.R. Insulin containing polyethylenimine-dextran sulfate nanoparticles. Int. J. Pharm. 255 (2003) 139-151
16. Rajaonarivony M., Vauthier C., Couarraze G., Puisieux F., and Couvreur P. Development of a new drug carrier made from alginate. J. Pharm. Sci. 82 (1993) 912-917
17. Sarmento B., Ribeiro A., Veiga F., Neufeld R., and Ferreira D. Insulin-loaded alginate/chitosan nanoparticles produced by ionotropic pre-gelation. Rev. Port. Farm. LII (2005) 139-140
18. Dong A., Huang P., and Caughey W.S. Protein secondary structures in water from second-derivative amide I infrared spectra. Biochemistry 29 (1990) 3303-3308
19. Sarmento B., Ribeiro A., Veiga F., and Ferreira D. Development and validation of a rapid reversed-phase HPLC method for the determination of insulin from nanoparticulate systems. Biomed. Chromatogr. 20 (2006) 898-903
20. Zheng C.-H., Gao J.-Q., Zhang Y.-P., and Liang W.-Q. A protein delivery system: biodegradable alginate-chitosan-poly(lactic-co-glycolic acid) composite microspheres. Biochem. Biophys. Res. Commun. 323 (2004) 1321-1327
21. Ribeiro A.J., Silva C., Ferreira D., and Veiga F. Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique. Eur. J. Pharm. Sci. 25 (2005) 31-40
22. Jørgensen L., Vermehren C., Bjerregaard S., and Froekjaer S. Secondary structure alterations in insulin and growth hormone water-in-oil emulsions. Int. J. Pharm. 254 (2003) 7-10
23. Nielsen L., Khurana R., Coats A., Frokjaer S., Brange J., Vyas S., Uversky V.N., and Fink A.L. Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism. Biochemistry 40 (2001) 6036-6046
24. Pocker Y., and Biswas S.B. Conformational dynamics of insulin in solution. Circular dichroic studies. Biochemistry 19 (1980) 4043-5049
25. Brange J., Andersen L., Laursen E.D., Meyn G., and Rasmussen E. Toward understanding insulin fibrillation. J. Pharm. Sci. 86 (1997) 517-525