A 3D collagen microsphere culture system for GDNF-secreting HEK293 cells with enhanced protein productivity

Biomaterials

Volumn 28, Issue 35, 2007, Pages 5369-5380

  Wong, Hoi Ling ^a   Wang, Ming Xi ^b   Cheung, Pik To ^b   Yao, Kwok Ming ^b   Chan, Barbara Pui ^a  

^a Department of Mechanical Engineering   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

3D culture; Biopharmaceutical manufacturing; Collagen; GDNF secreting HEK293 cells; Microencapsulation; Microsphere

Indexed keywords

BIOACTIVITY; CELL CULTURE; CELL GROWTH; CELL PROLIFERATION; COLLAGEN; METABOLISM;

BIOPHARMACEUTICAL MANUFACTURING; CELL CYCLE; HUMAN EMBRYONIC KIDNEY (HEK); MICROENCAPSULATION; NEUROTROPHIC FACTOR;

MICROSPHERES;

COLLAGEN; GLIAL CELL LINE DERIVED NEUROTROPHIC FACTOR; MICROSPHERE; PROTEIN;

ARTICLE; CELL COUNT; CELL CULTURE; CELL CYCLE; CELL GROWTH; CELL PROLIFERATION; CELL STRAIN HEK293; CELL VIABILITY; CONTROLLED STUDY; EMBRYO; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; COLLAGEN TYPE I; GLIAL CELL LINE-DERIVED NEUROTROPHIC FACTOR; HUMANS; KIDNEY; MICE; MICROSPHERES; PC12 CELLS; RATS; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 34948825863     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2007.08.016     Document Type: Article

References (43)

1. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22 11 (2004) 1393-1398
2. Levine D.W., Wong J.S., Wang D.I., and Thilly W.G. Microcarrier cell culture: new methods for research-scale application. Somatic Cell Genet 3 2 (1977) 149-155
3. Durrschmid M., Landauer K., Simic G., Bluml G., and Doblhoff-Dier O. Scalable inoculation strategies for microcarrier-based animal cell bioprocesses. Biotechnol Bioeng 83 6 (2003) 681-686
4. Geserick C., Bonarius H.P.J., Kongerslev L., Hauser H., and Mueller P.P. Enhanced productivity during controlled proliferation of BHK cells in continuously perfused bioreactors. Biotechol Bioeng 69 (2000) 266-274
5. Hirtenstein M., Clark J., Lindgren G., and Vretblad P. Microcarriers for animal cell culture: a brief review of theory and practice. Dev Biol Stand 46 (1980) 109-116
6. Voigt M., Andree C., Kalt T., Dormann S., Schaefer D.J., Walgenbach K.J., et al. Human recombinant EGF protein delivered by a biodegradable cell transplantation system. Tissue Eng 8 2 (2002) 263-272
7. Liu J.Y., Hafner J., Dragieva G., and Burg G. Bioreactor microcarrier cell culture system (Bio-MCCS) for large-scale production of autologous melanocytes. Cell Transplant 13 7-8 (2004) 809-816
8. Bancel S., and Hu W.S. Confocal laser scanning microscopy examination of cell distribution in macroporous microcarriers. Biotechnol Prog 12 3 (1996) 398-402
9. Overstreet M., Sohrabi A., Polotsky A., Hungerford D.S., and Frondoza C.G. Collagen microcarrier spinner culture promotes osteoblast proliferation and synthesis of matrix proteins. In Vitro Cell Dev Biol Anim 39 5-6 (2003) 228-234
10. Sanchez-Bustamante C.D., Kelm J.M., Mitta B., and Fussenegger M. Heterologous protein production capacity of mammalian cells cultivated as monolayers and microtissues. Biotechnol Bioeng 93 1 (2005) 169-180
11. Bailey JE, Fussenegger M, Renner WA. Cytostatic process increases the productivity of cultured cells, US Patent No. 6,274,341, 2001
12. Weber W., Rimann M., Spielmann M., Keller B., Daoud-El Baba M., Aubel D., et al. Gas-inducible transgene expression in mammalian cells and mice. Nat Biotechnol 22 11 (2004) 1440-1444
13. Kaufmann H., and Fussenegger M. Metabolic engineering of mammalian cells for higher protein yield. In: Makrides S.C. (Ed). Gene transfer and expression in mammalian cells (2003), Elsevier Science BV, Amsterdam 457-569
14. Suzuki E., and Ollis D.F. Enhanced antibody production at slowed growth rates: experimental demonstration and a simple structured model. Biotechnol Prog 6 3 (1990) 231-236
15. Jenkins N., and Hovey A. Temperature control of growth and productivity in mutant Chinese Hamster Ovary Cells synthesizing a recombinant protein. Biotechnol Bioeng 42 (1993) 1029-1036
16. Kastan M.B., Radin A.I., Kuerbitz S.J., Onyekwere O., Wolkow C.A., Civin C.I., et al. Levels of p53 protein increase with maturation in human hematopoietic cells. Cancer Res 51 16 (1991) 4279-4286
17. Watanabe S., Shttleworth J., and Al-Rubeai M. Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21. Biotechnol Bioeng 77 (2002) 1-7
18. Coats S., Flanagan W.M., Nourse J., and Roberts J.M. Requirement of p27Kip1 for restriction point control of the fibroblast cell cycle. Science 272 5263 (1996) 877-880
19. Mercille S., and Massie B. Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng 44 (1994) 1140-1154
20. Ko L.J., and Prives C. p53: puzzle and paradigm. Genes Dev 10 9 (1996) 1054-1072
21. Uludag H., De Vos P., and Tresco P.A. Technology of mammalian cell encapsulation. Adv Drug Deliv Rev 42 1-2 (2000) 29-64
22. Orive G., Hernandez R.M., Rodriguez Gascon A., Calafiore R., Chang T.M., de Vos P., et al. History, challenges and perspectives of cell microencapsulation. Trends Biotechnol 22 2 (2004) 87-92
23. Batorsky A., Liao J., Lund A.W., Plopper G.E., and Stegemann J.P. Encapsulation of adult human mesenchymal stem cells within collagen-agarose microenvironments. Biotechnol Bioeng 92 4 (2005) 492-500
24. Nuttelman C.R., Tripodi M.C., and Anseth K.S. Synthetic hydrogel niches that promote hMSC viability. Matrix Biol 24 3 (2005) 208-218
25. Grohn P., Klock G., and Zimmermann U. Collagen-coated Ba(2+)-alginate microcarriers for the culture of anchorage-dependent mammalian cells. Biotechniques 22 5 (1997) 970-975
26. Zimmermann H., Hillgartner M., Manz B., Feilen P., Brunnenmeier F., Leinfelder U., et al. Fabrication of homogeneously cross-linked, functional alginate microcapsules validated by NMR-, CLSM- and AFM-imaging. Biomaterials 24 12 (2003) 2083-2096
27. Yannas I.V. Natural materials. In: Ratner B.D., Hoffman A.S., Schoen F.J., and Lemons J.E. (Eds). Biomaterials sciences-an introduction to materials in medicine (1996), Academic Press, California 84-93
28. Chan BP, Chan GCF, Wong HL, Cheung PT, Chan D, Cheah K. Cell-matrix microsphere, associated products, methods for preparation and applications. Regular Patent Application No. 60/801,975 (filed on 19 May 2007).
29. Chan B.P., Hui T.Y., Yeung C.W., Li J., Mo I., and Chan G.C.F. Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine. Biomaterials 28 (2007) 4652-4666
30. Hui TY, K Cheung, D Chan, Chan BP. Chondrogenic differentiation of human mesenchymal stem cells in three-dimensional collagen/chondroitin-6-sulfate gels: influences of cell seeding density and collagen concentration. International Society for Stem Cell Research 5th Annual Meeting, Jun 15-21, 2007, Cairns, Queensland, Australia.
31. Wang MX, 2006. Production and characterization of recombinant mouse Pro-GDNF. PhD Thesis, The University of Hong Kong.
32. Grimm L., Holinski-Feder E., Teodoridis J., Scheffer B., Schindelhauer D., Meitinger T., et al. Analysis of the human GDNF gene reveals an inducible promoter, three exons, a triplet repeat within the 3′-UTR and alternative splice products. Hum Mol Genet 7 12 (1998) 1873-1886
33. Sun X., Zhang Y., Tan W., Zhou Y., and Hua P. Attachment kinetics of Vero cells onto CT-3 microcarriers. J Biosci Bioeng 90 1 (2000) 32-36
34. Werner N.S., Weber W., Fussenegger M., and Geisse S. A gas-inducible expression system in HEK.EBNA cells applied to controlled proliferation studies by expression of p27(Kip1). Biotechnol Bioeng. 96 6 (2007) 1155-1166
35. Ponce S., Orive G., Gascon A.R., Hernandez R.M., and Pedraz J.L. Microcapsules prepared with different biomaterials to immobilize GDNF secreting 3T3 fibroblasts. Int J Pharmaceutics 293 (2005) 1-10
36. Caumont A.S., Octave J.N., and Hermans E. Amantadine and memantine induce the expression of the glial cell line-derived neurotrophic factor in C6 glioma cells. Neurosci Lett 394 3 (2006) 196-201
37. Hashimoto M., Ito T., Fukumitsu H., Nomoto H., Furukawa Y., Furukawa S., et al. Stimulation of production of glial cell line-derived neurotrophic factor and nitric oxide by lipopolysaccharide with different dose-responsiveness in cultured rat macrophages. Biomed Res 26 5 (2005) 223-229
38. Nishiguchi M., Tokugawa K., Yamamoto K., Akama T., Nozawa Y., Chaki S., et al. Increase in secretion of glial cell line-derived neurotrophic factor from glial cell lines by inhibitors of vacuolar ATPase. Neurochem Int 42 6 (2003) 493-498
39. Brundin P. GDNF treatment in Parkinson's disease: time for controlled clinical trials?. Brain 125 10 (2002) 2149-2151
40. Alberch J., Perez-Navarro E., and Canals J.M. Neuroprotection by neurotrophins and GDNF family members in the excitotoxic model of Huntington's disease. Brain Res Bull 57 6 (2002) 817-822
41. Kirik D., Georgievska B., and Bjorklund A. Localized striatal delivery of GDNF as a treatment for Parkinson disease. Nat Neurosci 7 2 (2004) 105-110
42. Zurn A.D., Widmer H.R., and Aebischer P. Sustained delivery of GDNF: towards a treatment for Parkinson's disease. Brain Res Brain Res Rev 36 2-3 (2001) 222-229
43. Chan BP, Yeung CW, CW Wong, Chan GCF. Self-assembled collagen-human mesenchymal stem cell (hMSCs) microspheres-novel bio-devices for cell delivery. International Society for Stem Cell Research 5th Annual Meeting, June 15-21, 2007. Cairns, Queensland, Australia.