The EWS-Oct-4 fusion gene encodes a transforming gene

Biochemical Journal

Volumn 406, Issue 3, 2007, Pages 519-526

  Lee, Jungwoon ^a   Kim, Ja Young ^a   Kang, In Young ^a   Kim, Hye Kyoung ^a   Han, Yong Mahn ^b   Kim, Jungho ^a  

^a Sogang University   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Bone and soft tissue tumour; Chromosomal translocation; EWS Oct 4 fusion protein; Transactivation; Transformation

Indexed keywords

CHROMOSOMAL TRANSLOCATION; EWS-OCT-4 FUSION PROTEIN; SOFT TISSUE TUMORS; TRANSACTIVATION; TRANSFORMATION;

CHROMOSOMES; GENETIC ENGINEERING; ONCOLOGY; TISSUE;

GENE ENCODING;

CHIMERIC PROTEIN; FIBROBLAST GROWTH FACTOR 4; HYBRID PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN DERIVATIVE; PROTEIN NANOG; RNA BINDING PROTEIN EWS; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BINDING SITE; CHROMOSOME TRANSLOCATION 6; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE DELETION; GENETIC ANALYSIS; GENETIC TRANSFORMATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INDUCTION; REPORTER GENE; TRANSACTIVATION;

ANIMALS; CELL TRANSFORMATION, NEOPLASTIC; CHROMOSOMES, HUMAN, PAIR 22; CHROMOSOMES, HUMAN, PAIR 6; DNA-BINDING PROTEINS; EMBRYONIC STEM CELLS; GENE EXPRESSION REGULATION; HUMANS; MICE; MICE, NUDE; OCTAMER TRANSCRIPTION FACTOR-3; ONCOGENE PROTEINS, FUSION; ONCOGENES; RNA-BINDING PROTEIN EWS; SARCOMA, EWING'S; TRANSLOCATION, GENETIC;

MUS MUSCULUS;

EID: 34548720314     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070243     Document Type: Article

References (46)

1. Rabbitts, T. H. (1999) Perspective: chromosomal translocations can affect genes controlling gene expression and differentiation: why are these functions targeted? J. Pathol. 187, 39-42
2. Yamaguchi, S., Yamazaki, Y., Ishikawa, Y., Kawaguchi, N., Mukai, H. and Nakamura, T. (2005) EWSR1 is fused to POU5F1 in a bone tumor with translocation t(6;22)(p21;q12). Genes Chromosomes Cancer 43, 217-222
3. Kim, J. and Pelletier, J. (1999) Molecular genetics of chromosome translocations involving EWS and related family members. Physiol. Genomics 1, 127-138
4. Delattre, O., Zucman, J., Plougastel, B., Desmaze, C., Melot, T., Peter, M., Kovar, H. Joubert, I., de Jong, P., Rouleau, G. et al. (1992) Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature 359, 162-165
5. Scholer, H. R., Dressier, G. R., Balling, R., Rohdewohld, H. and Gruss, P. (1990) Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J. 9, 2185-2195
6. Okamoto, K., Okazawa, H., Okuda, A., Sakai, M., Muramatsu, M. and Hamada, H. (1990) A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell 60, 461-472
7. Rosner, M. H., Vigano, M. A., Ozato, K., Timmons, P. M., Poirier, F., Rigby, P. W. and Staudt, L. M. (1990) A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature 345, 686-692
8. Goto, T., Adjaye, J., Rodeck, C. H. and Monk, M. (1999) Identification of genes expressed in human primordial germ cells at the time of entry of the female germ line into meiosis. Mol. Hum. Reprod. 5, 851-860
9. Hansis, C., Grifo, J. A. and Krey, L. C. (2000) Oct-4 expression in inner cell mass and trophectoderm of human blastocysts. Mol. Hum. Reprod. 6, 999-1004
10. Burdon, T., Smith, A. and Savatier, P. (2002) Signalling, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol. 12, 432-438
11. Herr, W. and Cleary, M. A. (1995) The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain. Genes Dev 9, 1679-1693
12. Rodda, D. J., Chew, J. L., Lim, L. H., Loh, Y. H., Wang, B., Ng, H. H. and Robson, P. (2005) Transcriptional regulation of nanog by OCT4 and SOX2. J Biol. Chem. 280, 24731-24737
13. Lamb, K. A. and Rizzino, A. (1998) Effects of differentiation on the transcriptional regulation of the FGF-4 gene: critical roles played by a distal enhancer. Mol. Reprod. Dev. 51, 218-224
14. Ambrosetti, D. C., Basilico, C. and Dailey, L. (1997) Synergistic activation of the fibroblast growth factor 4 enhancer by Sox2 and Oct-3 depends on protein-protein interactions facilitated by a specific spatial arrangement of factor binding sites. Mol. Cell. Biol. 17, 6321-6329
15. Vigano, M. A. and Staudt, L. M. (1996) Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1. Nucleic Acids Res. 24, 2112-2118
16. Kuroda, T., Tada, M., Kubota, H., Kimura, H., Hatano, S. Y., Suemori, H., Nakatsuji, N. and Tada, T. (2005) Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol. Cell. Biol. 25, 2475-2485
17. Ezashi, T., Ghosh, D. and Roberts, R. M. (2001) Repression of Ets-2-induced transactivation of the tau interferon promoter by Oct-4. Mol. Cell. Biol. 21, 7883-7891
18. Liu, L., Leaman, D., Villalta, M. and Roberts, R. M. (1997) Silencing of the gene for the α-subunit of human chorionic gonadotropin by the embryonic transcription factor Oct-3/4. Mol. Endocrinol. 11, 1651-1658
19. Liu, L. and Roberts, R. M. (1996) Silencing of the gene for the β-subunit of human chorionic gonadotropin by the embryonic transcription factor Oct-3/4. J. Biol. Chem. 271, 16683-16689
20. Pesce, M. and Scholer, H. R. (2001) Oct-4: gatekeeper in the beginnings of mammalian development. Stem Cells 19, 271-278
21. Ovitt, C. E. and Scholer, H. R. (1998) The molecular biology of Oct-4 in the early mouse embryo. Mol. Hum. Reprod. 4, 1021-1031
22. Gidekel, S., Pizov, G., Bergman, Y. and Pikarsky, E. (2003) Oct-3/4 is a dose-dependent oncogenic fate determinant. Cancer Cell 4, 361-370
23. Looijenga, L. H., Stoop, H., de Leeuw, H. P., de Gouveia Brazao, C. A., Gillis, A. J., van Roozendaal, K. E., van Zoelen, E. J., Weber, R. F., Wolffenbuttel, K. P., van Dekken, H. et al. (2003) POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors. Cancer Res. 63, 2244-2250
24. Hellerstedt, B. A. and Pienta, K. J. (2003) Germ cell tumors: review of selected studies from 2002. Curr. Opin. Oncol. 15, 234-238
25. Oliver, R. T. (1999) Germ cell cancer. Curr. Opin. Oncol. 11, 236-241
26. Palumbo, C., van Roozendaal, K., Gillis, A. J., van Gurp, R. H., de Munnik, H., Oosterhuis, J. W., van Zoelen, E. J. and Looijenga, L. H. (2002) Expression of the PDGF α-receptor 1.5 kb transcript, OCT-4, and c-KIT in human normal and malignant tissues. Implications for the early diagnosis of testicular germ cell tumours and for our understanding of regulatory mechanisms. J. Pathol. 196, 467-477
27. Wang, P., Branch, D. R., Bali, M., Schultz, G. A., Goss, P. E. and Jin, T. (2003) The POU homeodomain protein OCT3 as a potential transcriptional activator for fibroblast growth factor-4 (FGF-4) in human breast cancer cells. Biochem. J. 375, 199-205
28. Monk, M. and Holding, C. (2001) Human embryonic genes re-expressed in cancer cells. Oncogene 20, 8085-8091
29. Jin, T., Branch, D. R., Zhang, X., Qi, S., Youngson, B. and Goss, P. E. (1999) Examination of POU homeobox gene expression in human breast cancer cells. Int. J. Cancer 81, 104-112
30. Hochedlinger, K., Yamada, Y., Beard, C. and Jaenisch, R. (2005) Ectopic expression of Oct-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues. Cell 121, 465-477
31. Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: a Laboratory Manual, Cold Spring Harbor Laboratory Press, New York
32. Lee, J., Kim, H. K., Rho, J. Y., Han, Y. M. and Kim, J. (2006) The human OCT-4 isoforms differ in their ability to confer self-renewal. J. Biol. Chem. 281, 33554-33565
33. Lee, J., Rhee, B. K., Bae, G. Y., Han, Y. M. and Kim, J. (2005) Stimulation of Oct-4 activity by Ewing's sarcoma protein. Stem Cells 23, 738-751
34. Kim, J., Lee, K. and Pelletier, J. (1998) The desmoplastic small round cell tumor t(11;22) translocation produces EWS/WT1 isoforms with differing oncogenic properties. Oncogene 16, 1973-1979
35. Botquin, V., Hess, H., Fuhrmann, G., Anastassiadis, C., Gross, M. K., Vriend, G. and Scholer, H. R. (1998) New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2. Genes Dev. 12, 2073-2090
36. Pan, G., Qin, B., Liu, N., Scholer, H. R. and Pel, D. (2004) Identification of a nuclear localization signal in OCT4 and generation of a dominant negative mutant by its ablation. J.Biol Chem. 279, 37013-37020
37. Kim, J., Lee, K. and Pelletier, J. (1998) The DNA binding domains of the WT1 tumor suppressor gene product and chimeric EWS/WT1 oncoprotein are functionally distinct. Oncogene 16, 1021-1030
38. Brown, A. D., Lopez-Terrada, D., Denny, C. and Lee, K. A. (1995) Promoters containing ATF-binding sites are de-regulated in cells that express the EWS/ATF1 oncogene. Oncogene 10, 1749-1756
39. Niwa, H., Miyazaki, J. and Smith, A. G. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24, 372-376
40. Rabbitts, T. H. and Stocks, M. R. (2003) Chromosomal translocation products engender new intracellular therapeutic technologies. Nat. Med. 9, 383-386
41. Baskaran, R., Dahmus, M. E. and Wang, J. Y. (1993) Tyrosine phosphorylation of mammalian RNA polymerase II carboxyl-terminal domain. Proc. Natl. Acad. Sci. U.S.A. 90, 11167-11171
42. O'Brien, T., Hardin, S., Greenleaf, A. and Lis, J. T. (1994) Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation. Nature 370, 75-77
43. Kim, J., Lee, J. M., Branton, P. E. and Pelletier, J. (1999) Modification of EWS/WT1 functional properties by phosphorylation. Proc. Natl. Acad. Sci. U.S.A. 96, 14300-14305
44. McLeskey, S. W., Kurebayashi, J., Honig, S. F., Zwiebel, J., Lippman, M. E., Dickson, R. B. and Kern, F. G. (1993) Fibroblast growth factor 4 transfection of MCF-7 cells produces cell lines that are tumorigenic and metastatic in ovariectomized or tamoxifen-treated athymic nude mice. Cancer Res. 53, 2168-2177
45. Souttou, B., Gamby, C., Crepin, M. and Hamelin, R. (1996) Tumoral progression of human breast epithelial cells secreting FGF2 and FGF4. Int. J. Cancer 68, 675-681
46. Piestun, D., Kochupurakkal, B. S., Jacob-Hirsch, J., Zeligson, S., Koudritsky, M., Domany, E., Amariglio, N., Rechavi, G. and Givol, D. (2006) Nanog transforms NIH3T3 cells and targets cell-type restricted genes. Biochem. Biophys. Res. Commun. 343, 279-285