Asymmetry in the structure of the ABC transporter - Binding protein complex BtuCD-BtuF

Science

Volumn 317, Issue 5843, 2007, Pages 1387-1390

  Hvorup, Rikki N ^a   Goetz, Birke A ^a   Niederer, Martina ^a   Hollenstein, Kaspar ^a   Perozo, Eduardo ^b   Locher, Kaspar P ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; BINDING PROTEIN; CYANOCOBALAMIN; CYSTEINE; MUTANT PROTEIN; PERIPLASMIC BINDING PROTEIN; PROTEIN SUBUNIT;

COMPARATIVE STUDY; ELECTRON SPIN RESONANCE; PROTEIN; VITAMIN;

ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; CYTOPLASM; ELECTRON SPIN RESONANCE; ESCHERICHIA COLI; MEMBRANE POTENTIAL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; PROTEIN TRANSPORT; PROTEOLIPOSOME; SPIN LABELING;

AMINO ACID SEQUENCE; ATP-BINDING CASSETTE TRANSPORTERS; CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 34548671159     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1145950     Document Type: Article

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27. We thank C. Schulze-Briese, T. Tomizaki, E. Pohl, and the beamline staff at the Swiss Light Source for assistance with data collection and B. Blattmann at the National Center for Excellence in Research (NCCR) Structural Biology Zurich for assistance with initial crystallization screening. This work was supported by the Swiss National Science Foundation, the Roche Research Fund, and the NCCR Structural Biology Zurich. Coordinates and structure factors for BtuCD-F have been deposited with the Protein Data Bank (www.rcsb.org/pdb) with the identification number 2QI9.