-
1
-
-
33746284200
-
Protein targeting to destinations of the secretory pathway in the malaria parasite Plasmodium falciparum
-
Tonkin C.J., Pearce J.A., McFadden G.I., and Cowman A.F. Protein targeting to destinations of the secretory pathway in the malaria parasite Plasmodium falciparum. Curr Opin Microbiol 9 (2006) 381-387
-
(2006)
Curr Opin Microbiol
, vol.9
, pp. 381-387
-
-
Tonkin, C.J.1
Pearce, J.A.2
McFadden, G.I.3
Cowman, A.F.4
-
2
-
-
33748491297
-
The cell biology of secondary endosymbiosis-how parasites build, divide and segregate the apicoplast
-
Vaishnava S., and Striepen B. The cell biology of secondary endosymbiosis-how parasites build, divide and segregate the apicoplast. Mol Microbiol 61 (2006) 1380-1387
-
(2006)
Mol Microbiol
, vol.61
, pp. 1380-1387
-
-
Vaishnava, S.1
Striepen, B.2
-
3
-
-
27944471039
-
Signal-mediated export of proteins from the malaria parasite to the host erythrocyte
-
Marti M., Baum J., Rug M., Tilley L., and Cowman A.F. Signal-mediated export of proteins from the malaria parasite to the host erythrocyte. J Cell Biol 171 (2005) 587-592
-
(2005)
J Cell Biol
, vol.171
, pp. 587-592
-
-
Marti, M.1
Baum, J.2
Rug, M.3
Tilley, L.4
Cowman, A.F.5
-
5
-
-
0037015614
-
Genome sequence of the human malaria parasite Plasmodium falciparum
-
Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419 (2002) 498-511
-
(2002)
Nature
, vol.419
, pp. 498-511
-
-
Gardner, M.J.1
Hall, N.2
Fung, E.3
White, O.4
Berriman, M.5
Hyman, R.W.6
Carlton, J.M.7
Pain, A.8
Nelson, K.E.9
Bowman, S.10
-
6
-
-
0037015592
-
Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii
-
Carlton J.M., Angiuoli S.V., Suh B.B., Kooij T.W., Pertea M., Silva J.C., Ermolaeva M.D., Allen J.E., Selengut J.D., Koo H.L., et al. Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoelii. Nature 419 (2002) 512-519
-
(2002)
Nature
, vol.419
, pp. 512-519
-
-
Carlton, J.M.1
Angiuoli, S.V.2
Suh, B.B.3
Kooij, T.W.4
Pertea, M.5
Silva, J.C.6
Ermolaeva, M.D.7
Allen, J.E.8
Selengut, J.D.9
Koo, H.L.10
-
7
-
-
33646559434
-
Plasmodium post-genomics: better the bug you know?
-
Kooij T.W., Janse C.J., and Waters A.P. Plasmodium post-genomics: better the bug you know?. Nat Rev Microbiol 4 (2006) 344-357
-
(2006)
Nat Rev Microbiol
, vol.4
, pp. 344-357
-
-
Kooij, T.W.1
Janse, C.J.2
Waters, A.P.3
-
8
-
-
1642396715
-
Toxoplasma as a novel system for motility
-
Soldati D., and Meissner M. Toxoplasma as a novel system for motility. Curr Opin Cell Biol 16 (2004) 32-40
-
(2004)
Curr Opin Cell Biol
, vol.16
, pp. 32-40
-
-
Soldati, D.1
Meissner, M.2
-
9
-
-
1542285447
-
Toxoplasma gondii: the model apicomplexan
-
Kim K., and Weiss L.M. Toxoplasma gondii: the model apicomplexan. Int J Parasitol 34 (2004) 423-432
-
(2004)
Int J Parasitol
, vol.34
, pp. 423-432
-
-
Kim, K.1
Weiss, L.M.2
-
10
-
-
0027310836
-
Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii
-
Soldati D., and Boothroyd J.C. Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii. Science 260 (1993) 349-352
-
(1993)
Science
, vol.260
, pp. 349-352
-
-
Soldati, D.1
Boothroyd, J.C.2
-
11
-
-
0031255827
-
Tagging genes and trapping promoters in Toxoplasma gondii by insertional mutagenesis
-
Roos D.S., Sullivan W.J., Striepen B., Bohne W., and Donald R.G. Tagging genes and trapping promoters in Toxoplasma gondii by insertional mutagenesis. Methods 13 (1997) 112-122
-
(1997)
Methods
, vol.13
, pp. 112-122
-
-
Roos, D.S.1
Sullivan, W.J.2
Striepen, B.3
Bohne, W.4
Donald, R.G.5
-
12
-
-
0027136119
-
Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria
-
Donald R.G., and Roos D.S. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proc Natl Acad Sci U S A 90 (1993) 11703-11707
-
(1993)
Proc Natl Acad Sci U S A
, vol.90
, pp. 11703-11707
-
-
Donald, R.G.1
Roos, D.S.2
-
13
-
-
0029610921
-
Restriction enzyme-mediated intergration elevates trabnsformation frequency and enables co-transfection of Toxoplasma gondii
-
Black M., Seeber F., Soldati D., Kim K., and Boothroyd J.C. Restriction enzyme-mediated intergration elevates trabnsformation frequency and enables co-transfection of Toxoplasma gondii. Mol. Biochem. Parasitol. 74 (1995) 55-63
-
(1995)
Mol. Biochem. Parasitol.
, vol.74
, pp. 55-63
-
-
Black, M.1
Seeber, F.2
Soldati, D.3
Kim, K.4
Boothroyd, J.C.5
-
14
-
-
0028211397
-
Homologous recombination and gene replacement at the dihydrofolate reductase-thymidylate synthase locus in Toxoplasma gondii
-
Donald R.G., and Roos D.S. Homologous recombination and gene replacement at the dihydrofolate reductase-thymidylate synthase locus in Toxoplasma gondii. Mol Biochem Parasitol 63 (1994) 243-253
-
(1994)
Mol Biochem Parasitol
, vol.63
, pp. 243-253
-
-
Donald, R.G.1
Roos, D.S.2
-
15
-
-
0034907083
-
Negative selection of herpes simplex virus thymidine kinase in Toxoplasma gondii
-
Fox B.A., Belperron A.A., and Bzik D.J. Negative selection of herpes simplex virus thymidine kinase in Toxoplasma gondii. Mol Biochem Parasitol 116 (2001) 85-88
-
(2001)
Mol Biochem Parasitol
, vol.116
, pp. 85-88
-
-
Fox, B.A.1
Belperron, A.A.2
Bzik, D.J.3
-
16
-
-
0032145890
-
A cell cycle model for the tachyzoite of Toxoplasma gondii using the Herpes simplex virus thymidine kinase
-
Radke J.R., and White M.W. A cell cycle model for the tachyzoite of Toxoplasma gondii using the Herpes simplex virus thymidine kinase. Mol Biochem Parasitol 94 (1998) 237-247
-
(1998)
Mol Biochem Parasitol
, vol.94
, pp. 237-247
-
-
Radke, J.R.1
White, M.W.2
-
17
-
-
0032521445
-
Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis
-
Donald R.G., and Roos D.S. Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis. Mol Biochem Parasitol 91 (1998) 295-305
-
(1998)
Mol Biochem Parasitol
, vol.91
, pp. 295-305
-
-
Donald, R.G.1
Roos, D.S.2
-
18
-
-
33748353213
-
-
Mazumdar J, E HW, Masek K, C AH, Striepen B: Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii. Proc Natl Acad Sci U S A 2006, 103:13192-13197. In order to obtain a conditional mutant for acyl carrier protein (ACP) an essential gene localised in the apicoplast, the authors applied a novel selection strategy based on positive/negative selection and succeeded to demonstrate the metabolic role of the apicoplast for the growth and virulence of T.gondii.
-
-
-
-
19
-
-
0030049909
-
Expression of a Plasmodium gene introduced into subtelomeric regions of Plasmodium berghei chromosomes
-
van Dijk M.R., Janse C.J., and Waters A.P. Expression of a Plasmodium gene introduced into subtelomeric regions of Plasmodium berghei chromosomes. Science 271 (1996) 662-665
-
(1996)
Science
, vol.271
, pp. 662-665
-
-
van Dijk, M.R.1
Janse, C.J.2
Waters, A.P.3
-
20
-
-
0029071447
-
Stable transfection of malaria parasite blood stages
-
van Dijk M.R., Waters A.P., and Janse C.J. Stable transfection of malaria parasite blood stages. Science 268 (1995) 1358-1362
-
(1995)
Science
, vol.268
, pp. 1358-1362
-
-
van Dijk, M.R.1
Waters, A.P.2
Janse, C.J.3
-
22
-
-
0030035041
-
Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine
-
Wu Y., Kirkman L.A., and Wellems T.E. Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad Sci U S A 93 (1996) 1130-1134
-
(1996)
Proc Natl Acad Sci U S A
, vol.93
, pp. 1130-1134
-
-
Wu, Y.1
Kirkman, L.A.2
Wellems, T.E.3
-
23
-
-
0028985140
-
Transfection of Plasmodium falciparum within human red blood cells
-
Wu Y., Sifri C.D., Lei H.H., Su X.Z., and Wellems T.E. Transfection of Plasmodium falciparum within human red blood cells. Proc Natl Acad Sci U S A 92 (1995) 973-977
-
(1995)
Proc Natl Acad Sci U S A
, vol.92
, pp. 973-977
-
-
Wu, Y.1
Sifri, C.D.2
Lei, H.H.3
Su, X.Z.4
Wellems, T.E.5
-
24
-
-
28044470105
-
High efficiency transfection of Plasmodium berghei facilitates novel selection procedures
-
Janse C.J., Franke-Fayard B., Mair G.R., Ramesar J., Thiel C., Engelmann S., Matuschewski K., van Gemert G.J., Sauerwein R.W., and Waters A.P. High efficiency transfection of Plasmodium berghei facilitates novel selection procedures. Mol Biochem Parasitol 145 (2006) 60-70
-
(2006)
Mol Biochem Parasitol
, vol.145
, pp. 60-70
-
-
Janse, C.J.1
Franke-Fayard, B.2
Mair, G.R.3
Ramesar, J.4
Thiel, C.5
Engelmann, S.6
Matuschewski, K.7
van Gemert, G.J.8
Sauerwein, R.W.9
Waters, A.P.10
-
25
-
-
34247507377
-
High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei
-
The authors describe a novel, highly efficient transfection technology that allows easy access for genome manipulation and establishment of transgenic parasites.
-
Janse C.J., Ramesar J., and Waters A.P. High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei. Nat Protoc 1 (2006) 346-356. The authors describe a novel, highly efficient transfection technology that allows easy access for genome manipulation and establishment of transgenic parasites.
-
(2006)
Nat Protoc
, vol.1
, pp. 346-356
-
-
Janse, C.J.1
Ramesar, J.2
Waters, A.P.3
-
26
-
-
0036500346
-
A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes
-
O'Donnell R.A., Freitas-Junior L.H., Preiser P.R., Williamson D.H., Duraisingh M., McElwain T.F., Scherf A., Cowman A.F., and Crabb B.S. A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes. EMBO J 21 (2002) 1231-1239
-
(2002)
EMBO J
, vol.21
, pp. 1231-1239
-
-
O'Donnell, R.A.1
Freitas-Junior, L.H.2
Preiser, P.R.3
Williamson, D.H.4
Duraisingh, M.5
McElwain, T.F.6
Scherf, A.7
Cowman, A.F.8
Crabb, B.S.9
-
27
-
-
0035253776
-
An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites
-
O'Donnell R.A., Preiser P.R., Williamson D.H., Moore P.W., Cowman A.F., and Crabb B.S. An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites. Nucleic Acids Res 29 (2001) 716-724
-
(2001)
Nucleic Acids Res
, vol.29
, pp. 716-724
-
-
O'Donnell, R.A.1
Preiser, P.R.2
Williamson, D.H.3
Moore, P.W.4
Cowman, A.F.5
Crabb, B.S.6
-
28
-
-
0030006717
-
Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum
-
Crabb B.S., and Cowman A.F. Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci U S A 93 (1996) 7289-7294
-
(1996)
Proc Natl Acad Sci U S A
, vol.93
, pp. 7289-7294
-
-
Crabb, B.S.1
Cowman, A.F.2
-
29
-
-
0030885188
-
Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil
-
Fidock D.A., and Wellems T.E. Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A 94 (1997) 10931-10936
-
(1997)
Proc Natl Acad Sci U S A
, vol.94
, pp. 10931-10936
-
-
Fidock, D.A.1
Wellems, T.E.2
-
30
-
-
0035253752
-
Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes
-
Deitsch K., Driskill C., and Wellems T. Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes. Nucleic Acids Res 29 (2001) 850-853
-
(2001)
Nucleic Acids Res
, vol.29
, pp. 850-853
-
-
Deitsch, K.1
Driskill, C.2
Wellems, T.3
-
31
-
-
33746425864
-
Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase
-
This system short-circuits the period of episomal replication and achieves rapid and (most importantly) irreversible integration of the plasmid DNA. This is done by encoding an integrase from the mycobacterial phage Bxb1 in the transfected plasmid to facilitate site-specific integration into the genome of the parasite.
-
Nkrumah L.J., Muhle R.A., Moura P.A., Ghosh P., Hatfull G.F., Jacobs Jr. W.R., and Fidock D.A. Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase. Nat Methods 3 (2006) 615-621. This system short-circuits the period of episomal replication and achieves rapid and (most importantly) irreversible integration of the plasmid DNA. This is done by encoding an integrase from the mycobacterial phage Bxb1 in the transfected plasmid to facilitate site-specific integration into the genome of the parasite.
-
(2006)
Nat Methods
, vol.3
, pp. 615-621
-
-
Nkrumah, L.J.1
Muhle, R.A.2
Moura, P.A.3
Ghosh, P.4
Hatfull, G.F.5
Jacobs Jr., W.R.6
Fidock, D.A.7
-
32
-
-
33845988781
-
A new system for rapid plasmid integration in Plasmodium parasites
-
Crabb B.S., and Gilson P.R. A new system for rapid plasmid integration in Plasmodium parasites. Trends Microbiol 15 (2007) 3-6
-
(2007)
Trends Microbiol
, vol.15
, pp. 3-6
-
-
Crabb, B.S.1
Gilson, P.R.2
-
33
-
-
33845626667
-
Advancements in transfection technologies for Plasmodium
-
Balu B., and Adams J.H. Advancements in transfection technologies for Plasmodium. Int J Parasitol 37 (2007) 1-10
-
(2007)
Int J Parasitol
, vol.37
, pp. 1-10
-
-
Balu, B.1
Adams, J.H.2
-
34
-
-
28044457006
-
High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac
-
This system is the first that allows widespread, and relatively random, direct integration into the genome of P.falciparum. Therefore this system can be the basis for forward genetic screen to isolate genes involved in certain processes during the life cycle of the parasite.
-
Balu B., Shoue D.A., Fraser Jr. M.J., and Adams J.H. High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac. Proc Natl Acad Sci U S A 102 (2005) 16391-16396. This system is the first that allows widespread, and relatively random, direct integration into the genome of P.falciparum. Therefore this system can be the basis for forward genetic screen to isolate genes involved in certain processes during the life cycle of the parasite.
-
(2005)
Proc Natl Acad Sci U S A
, vol.102
, pp. 16391-16396
-
-
Balu, B.1
Shoue, D.A.2
Fraser Jr., M.J.3
Adams, J.H.4
-
35
-
-
0036049784
-
Double-stranded RNA-mediatedgene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum
-
Malhotra P., Dasaradhi P.V., Kumar A., Mohmmed A., Agrawal N., Bhatnagar R.K., and Chauhan V.S. Double-stranded RNA-mediatedgene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum. Mol Microbiol 45 (2002) 1245-1254
-
(2002)
Mol Microbiol
, vol.45
, pp. 1245-1254
-
-
Malhotra, P.1
Dasaradhi, P.V.2
Kumar, A.3
Mohmmed, A.4
Agrawal, N.5
Bhatnagar, R.K.6
Chauhan, V.S.7
-
36
-
-
0036155407
-
RNA interference (RNAi) inhibits growth of Plasmodium falciparum
-
McRobert L., and McConkey G.A. RNA interference (RNAi) inhibits growth of Plasmodium falciparum. Mol Biochem Parasitol 119 (2002) 273-278
-
(2002)
Mol Biochem Parasitol
, vol.119
, pp. 273-278
-
-
McRobert, L.1
McConkey, G.A.2
-
37
-
-
33746280248
-
Single argonaute protein from Toxoplasma gondii is involved in the double-stranded RNA induced gene silencing
-
Al Riyahi A., Al-Anouti F., Al-Rayes M., and Ananvoranich S. Single argonaute protein from Toxoplasma gondii is involved in the double-stranded RNA induced gene silencing. Int J Parasitol 36 (2006) 1003-1014
-
(2006)
Int J Parasitol
, vol.36
, pp. 1003-1014
-
-
Al Riyahi, A.1
Al-Anouti, F.2
Al-Rayes, M.3
Ananvoranich, S.4
-
38
-
-
0036696519
-
Comparative analysis of antisense RNA, double-stranded RNA, and delta ribozyme-mediated gene regulation in Toxoplasma gondii
-
Al-Anouti F., and Ananvoranich S. Comparative analysis of antisense RNA, double-stranded RNA, and delta ribozyme-mediated gene regulation in Toxoplasma gondii. Antisense Nucleic Acid Drug Dev 12 (2002) 275-281
-
(2002)
Antisense Nucleic Acid Drug Dev
, vol.12
, pp. 275-281
-
-
Al-Anouti, F.1
Ananvoranich, S.2
-
39
-
-
12344305405
-
PfMyb1, a Plasmodium falciparum transcription factor, is required for intra-erythrocytic growth and controls key genes for cell cycle regulation
-
Gissot M., Briquet S., Refour P., Boschet C., and Vaquero C. PfMyb1, a Plasmodium falciparum transcription factor, is required for intra-erythrocytic growth and controls key genes for cell cycle regulation. J Mol Biol 346 (2005) 29-42
-
(2005)
J Mol Biol
, vol.346
, pp. 29-42
-
-
Gissot, M.1
Briquet, S.2
Refour, P.3
Boschet, C.4
Vaquero, C.5
-
40
-
-
2942592064
-
Plasmodium falciparum cysteine protease falcipain-1 is not essential in erythrocytic stage malaria parasites
-
Sijwali P.S., Kato K., Seydel K.B., Gut J., Lehman J., Klemba M., Goldberg D.E., Miller L.H., and Rosenthal P.J. Plasmodium falciparum cysteine protease falcipain-1 is not essential in erythrocytic stage malaria parasites. Proc Natl Acad Sci U S A 101 (2004) 8721-8726
-
(2004)
Proc Natl Acad Sci U S A
, vol.101
, pp. 8721-8726
-
-
Sijwali, P.S.1
Kato, K.2
Seydel, K.B.3
Gut, J.4
Lehman, J.5
Klemba, M.6
Goldberg, D.E.7
Miller, L.H.8
Rosenthal, P.J.9
-
42
-
-
0041927746
-
RNAi in protozoan parasites: what hope for the Apicomplexa?
-
Blackman M.J. RNAi in protozoan parasites: what hope for the Apicomplexa?. Protist 154 (2003) 177-180
-
(2003)
Protist
, vol.154
, pp. 177-180
-
-
Blackman, M.J.1
-
43
-
-
0037228729
-
High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein
-
Gubbels M.J., and Striepen B. High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein. Antimicrob Agents Chemother 47 (2003) 309-316
-
(2003)
Antimicrob Agents Chemother
, vol.47
, pp. 309-316
-
-
Gubbels, M.J.1
Striepen, B.2
-
44
-
-
0030826746
-
Use of Toxoplasma gondii expressing beta-galactosidase for colorimetric assessment of drug activity in vitro
-
McFadden D.C., Seeber F., and Boothroyd J.C. Use of Toxoplasma gondii expressing beta-galactosidase for colorimetric assessment of drug activity in vitro. Antimicrob Agents Chemother 41 (1997) 1849-1853
-
(1997)
Antimicrob Agents Chemother
, vol.41
, pp. 1849-1853
-
-
McFadden, D.C.1
Seeber, F.2
Boothroyd, J.C.3
-
45
-
-
0029670076
-
Escherichia coli beta-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii
-
Seeber F., and Boothroyd J.C. Escherichia coli beta-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii. Gene 169 (1996) 39-45
-
(1996)
Gene
, vol.169
, pp. 39-45
-
-
Seeber, F.1
Boothroyd, J.C.2
-
46
-
-
30344466984
-
Laser scanning cytometer-based assays for measuring host cell attachment and invasion by the human pathogen Toxoplasma gondii
-
Mital J., Schwarz J., Taatjes D.J., and Ward G.E. Laser scanning cytometer-based assays for measuring host cell attachment and invasion by the human pathogen Toxoplasma gondii. Cytometry A 69 (2006) 13-19
-
(2006)
Cytometry A
, vol.69
, pp. 13-19
-
-
Mital, J.1
Schwarz, J.2
Taatjes, D.J.3
Ward, G.E.4
-
47
-
-
0034770656
-
Isolation and characterization of a cold-sensitive attachment/invasion mutant of Toxoplasma gondii
-
Uyetake L., Ortega-Barria E., and Boothroyd J.C. Isolation and characterization of a cold-sensitive attachment/invasion mutant of Toxoplasma gondii. Exp Parasitol 97 (2001) 55-59
-
(2001)
Exp Parasitol
, vol.97
, pp. 55-59
-
-
Uyetake, L.1
Ortega-Barria, E.2
Boothroyd, J.C.3
-
48
-
-
0034458967
-
Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in Egress
-
Black M., Arrizabalaga G., and Boothroyd J.C. Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in Egress. Mol Cell Biol 20 (2000) 9399-9408
-
(2000)
Mol Cell Biol
, vol.20
, pp. 9399-9408
-
-
Black, M.1
Arrizabalaga, G.2
Boothroyd, J.C.3
-
49
-
-
0036096540
-
Identification and characterization of differentiation mutants in the protozoan parasite Toxoplasma gondii
-
Matrajt M., Donald R.G., Singh U., and Roos D.S. Identification and characterization of differentiation mutants in the protozoan parasite Toxoplasma gondii. Mol Microbiol 44 (2002) 735-747
-
(2002)
Mol Microbiol
, vol.44
, pp. 735-747
-
-
Matrajt, M.1
Donald, R.G.2
Singh, U.3
Roos, D.S.4
-
50
-
-
0036096929
-
Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction
-
Singh U., Brewer J.L., and Boothroyd J.C. Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction. Mol Microbiol 44 (2002) 721-733
-
(2002)
Mol Microbiol
, vol.44
, pp. 721-733
-
-
Singh, U.1
Brewer, J.L.2
Boothroyd, J.C.3
-
51
-
-
0031929681
-
Isolation of developmentally regulated genes from Toxoplasma gondii by a gene trap with the positive and negative selectable marker hypoxanthine-xanthine-guanine phosphoribosyltransferase
-
Knoll L.J., and Boothroyd J.C. Isolation of developmentally regulated genes from Toxoplasma gondii by a gene trap with the positive and negative selectable marker hypoxanthine-xanthine-guanine phosphoribosyltransferase. Mol Cell Biol 18 (1998) 807-814
-
(1998)
Mol Cell Biol
, vol.18
, pp. 807-814
-
-
Knoll, L.J.1
Boothroyd, J.C.2
-
52
-
-
25444466471
-
Disruption of a locus encoding a nucleolar zinc finger protein decreases tachyzoite-to-bradyzoite differentiation in Toxoplasma gondii
-
The authors applied random insertion mutagenesis to identify factors essential for differentiation of T.gondii.
-
Vanchinathan P., Brewer J.L., Harb O.S., Boothroyd J.C., and Singh U. Disruption of a locus encoding a nucleolar zinc finger protein decreases tachyzoite-to-bradyzoite differentiation in Toxoplasma gondii. Infect Immun 73 (2005) 6680-6688. The authors applied random insertion mutagenesis to identify factors essential for differentiation of T.gondii.
-
(2005)
Infect Immun
, vol.73
, pp. 6680-6688
-
-
Vanchinathan, P.1
Brewer, J.L.2
Harb, O.S.3
Boothroyd, J.C.4
Singh, U.5
-
53
-
-
3342905330
-
A GFP-based motif-trap reveals a novel mechanism of targeting for the Toxoplasma ROP4 protein
-
This Shows a technique that combines the power of tagging proteins with fluorescence markers with random insertion mutagenesis to identify novel proteins localised to certain organelles/structures within the parasite.
-
Bradley P.J., Li N., and Boothroyd J.C. A GFP-based motif-trap reveals a novel mechanism of targeting for the Toxoplasma ROP4 protein. Mol Biochem Parasitol 137 (2004) 111-120. This Shows a technique that combines the power of tagging proteins with fluorescence markers with random insertion mutagenesis to identify novel proteins localised to certain organelles/structures within the parasite.
-
(2004)
Mol Biochem Parasitol
, vol.137
, pp. 111-120
-
-
Bradley, P.J.1
Li, N.2
Boothroyd, J.C.3
-
54
-
-
3343008962
-
Fluorescent protein tagging in Toxoplasma gondii: identification of a novel inner membrane complex component conserved among Apicomplexa
-
This Shows a technique that combines the power of tagging proteins with fluorescence markers with random insertion mutagenesis to identify novel proteins localised to certain organelles/structures within the parasite.
-
Gubbels M.J., Wieffer M., and Striepen B. Fluorescent protein tagging in Toxoplasma gondii: identification of a novel inner membrane complex component conserved among Apicomplexa. Mol Biochem Parasitol 137 (2004) 99-110. This Shows a technique that combines the power of tagging proteins with fluorescence markers with random insertion mutagenesis to identify novel proteins localised to certain organelles/structures within the parasite.
-
(2004)
Mol Biochem Parasitol
, vol.137
, pp. 99-110
-
-
Gubbels, M.J.1
Wieffer, M.2
Striepen, B.3
-
55
-
-
0017081813
-
Toxoplasma gondii: isolation and preliminary characterization of temperature-sensitive mutants
-
Pfefferkorn E.R., and Pfefferkorn L.C. Toxoplasma gondii: isolation and preliminary characterization of temperature-sensitive mutants. Exp Parasitol 39 (1976) 365-376
-
(1976)
Exp Parasitol
, vol.39
, pp. 365-376
-
-
Pfefferkorn, E.R.1
Pfefferkorn, L.C.2
-
56
-
-
0037197911
-
Genetic complementation in apicomplexan parasites
-
The authors established a complementation system that allows to identify genes affected in mutant parasites, like for example temperature sensitive mutants.
-
Striepen B., White M.W., Li C., Guerini M.N., Malik S.B., Logsdon Jr. J.M., Liu C., and Abrahamsen M.S. Genetic complementation in apicomplexan parasites. Proc Natl Acad Sci U S A 99 (2002) 6304-6309. The authors established a complementation system that allows to identify genes affected in mutant parasites, like for example temperature sensitive mutants.
-
(2002)
Proc Natl Acad Sci U S A
, vol.99
, pp. 6304-6309
-
-
Striepen, B.1
White, M.W.2
Li, C.3
Guerini, M.N.4
Malik, S.B.5
Logsdon Jr., J.M.6
Liu, C.7
Abrahamsen, M.S.8
-
57
-
-
14544275891
-
Genetic rescue of a Toxoplasma gondii conditional cell cycle mutant
-
White M.W., Jerome M.E., Vaishnava S., Guerini M., Behnke M., and Striepen B. Genetic rescue of a Toxoplasma gondii conditional cell cycle mutant. Mol Microbiol 55 (2005) 1060-1071
-
(2005)
Mol Microbiol
, vol.55
, pp. 1060-1071
-
-
White, M.W.1
Jerome, M.E.2
Vaishnava, S.3
Guerini, M.4
Behnke, M.5
Striepen, B.6
-
58
-
-
32944458099
-
Invasion of red blood cells by malaria parasites
-
Cowman A.F., and Crabb B.S. Invasion of red blood cells by malaria parasites. Cell 124 (2006) 755-766
-
(2006)
Cell
, vol.124
, pp. 755-766
-
-
Cowman, A.F.1
Crabb, B.S.2
-
59
-
-
29144445901
-
Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis
-
The authors describe a novel approach to identify essential genes in the asexual stages of P. berghei. The mutagenesis of large genomic sequences is performed in E. coli based on transposon mutagenesis. Upon homologoues recombination absent mutants in the transfected P. berghei population indicate an essential function of the respective gene.
-
Sakamoto H., Thiberge S., Akerman S., Janse C.J., Carvalho T.G., and Menard R. Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis. Nucleic Acids Res 33 (2005) e174. The authors describe a novel approach to identify essential genes in the asexual stages of P. berghei. The mutagenesis of large genomic sequences is performed in E. coli based on transposon mutagenesis. Upon homologoues recombination absent mutants in the transfected P. berghei population indicate an essential function of the respective gene.
-
(2005)
Nucleic Acids Res
, vol.33
-
-
Sakamoto, H.1
Thiberge, S.2
Akerman, S.3
Janse, C.J.4
Carvalho, T.G.5
Menard, R.6
-
60
-
-
6944252213
-
Conditional mutagenesis using site-specific recombination in Plasmodium berghei
-
First conditional mutagenesis described in Plasmodium. The temporal control of the site-specific recombinase Flp was achieved by introducing this enzyme via Mendelian crosses in the mosquito.
-
Carvalho T.G., Thiberge S., Sakamoto H., and Menard R. Conditional mutagenesis using site-specific recombination in Plasmodium berghei. Proc Natl Acad Sci U S A 101 (2004) 14931-14936. First conditional mutagenesis described in Plasmodium. The temporal control of the site-specific recombinase Flp was achieved by introducing this enzyme via Mendelian crosses in the mosquito.
-
(2004)
Proc Natl Acad Sci U S A
, vol.101
, pp. 14931-14936
-
-
Carvalho, T.G.1
Thiberge, S.2
Sakamoto, H.3
Menard, R.4
-
61
-
-
14544291540
-
Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators
-
Establishment of a ectopic gene regulation system, based on Tetracycline-inducible transcriptional activation in P.falciparum that allows regulated expression of dominant negative genes and might facilitate generation of conditional mutants for essential genes.
-
Meissner M., Krejany E., Gilson P.R., de Koning-Ward T.F., Soldati D., and Crabb B.S. Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators. Proc Natl Acad Sci U S A 102 (2005) 2980-2985. Establishment of a ectopic gene regulation system, based on Tetracycline-inducible transcriptional activation in P.falciparum that allows regulated expression of dominant negative genes and might facilitate generation of conditional mutants for essential genes.
-
(2005)
Proc Natl Acad Sci U S A
, vol.102
, pp. 2980-2985
-
-
Meissner, M.1
Krejany, E.2
Gilson, P.R.3
de Koning-Ward, T.F.4
Soldati, D.5
Crabb, B.S.6
-
62
-
-
0037174664
-
Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion
-
Describes the first tight and efficient ectopic gene regulation system in an apicomplexan parasite. Using a forward genetic screen functional tet-transactivators have been isolated. The authors also demonstrate the efficiency of this system by generating a conditional mutant for the essential motorprotein MyosinA.
-
Meissner M., Schluter D., and Soldati D. Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion. Science 298 (2002) 837-840. Describes the first tight and efficient ectopic gene regulation system in an apicomplexan parasite. Using a forward genetic screen functional tet-transactivators have been isolated. The authors also demonstrate the efficiency of this system by generating a conditional mutant for the essential motorprotein MyosinA.
-
(2002)
Science
, vol.298
, pp. 837-840
-
-
Meissner, M.1
Schluter, D.2
Soldati, D.3
-
63
-
-
0346503889
-
Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice
-
Branda C.S., and Dymecki S.M. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice. Dev Cell 6 (2004) 7-28
-
(2004)
Dev Cell
, vol.6
, pp. 7-28
-
-
Branda, C.S.1
Dymecki, S.M.2
-
64
-
-
0029164598
-
Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase
-
Metzger D., Clifford J., Chiba H., and Chambon P. Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase. Proc Natl Acad Sci U S A 92 (1995) 6991-6995
-
(1995)
Proc Natl Acad Sci U S A
, vol.92
, pp. 6991-6995
-
-
Metzger, D.1
Clifford, J.2
Chiba, H.3
Chambon, P.4
-
65
-
-
0033059054
-
Genome engineering of toxoplasma gondii using the site-specific recombinase Cre [In Process Citation]
-
Brecht S., Erdhart H., Soete M., and Soldati D. Genome engineering of toxoplasma gondii using the site-specific recombinase Cre [In Process Citation]. Gene 234 (1999) 239-247
-
(1999)
Gene
, vol.234
, pp. 239-247
-
-
Brecht, S.1
Erdhart, H.2
Soete, M.3
Soldati, D.4
-
66
-
-
24344439282
-
Conditional expression of Toxoplasma gondii apical membrane antigen-1 (TgAMA1) demonstrates that TgAMA1 plays a critical role in host cell invasion
-
Application of conditional mutagenesis in T.gondii that led to the detailed functional characterisation of the vaccine candidate TgAMA-1 (homologoue of PfAMA-1) and TgMIC2 (homologoue of TRAP).
-
Mital J., Meissner M., Soldati D., and Ward G.E. Conditional expression of Toxoplasma gondii apical membrane antigen-1 (TgAMA1) demonstrates that TgAMA1 plays a critical role in host cell invasion. Mol Biol Cell 16 (2005) 4341-4349. Application of conditional mutagenesis in T.gondii that led to the detailed functional characterisation of the vaccine candidate TgAMA-1 (homologoue of PfAMA-1) and TgMIC2 (homologoue of TRAP).
-
(2005)
Mol Biol Cell
, vol.16
, pp. 4341-4349
-
-
Mital, J.1
Meissner, M.2
Soldati, D.3
Ward, G.E.4
-
67
-
-
33748066307
-
Toxoplasma MIC2 is a major determinant of invasion and virulence
-
Application of conditional mutagenesis in T. gondii that led to the detailed functional characterisation of the vaccine candidate TgAMA-1 (homologoue of PfAMA-1) and TgMIC2 (homologoue of TRAP).
-
Huynh M.H., and Carruthers V.B. Toxoplasma MIC2 is a major determinant of invasion and virulence. PLoS Pathog (2006) 2. Application of conditional mutagenesis in T. gondii that led to the detailed functional characterisation of the vaccine candidate TgAMA-1 (homologoue of PfAMA-1) and TgMIC2 (homologoue of TRAP).
-
(2006)
PLoS Pathog
, pp. 2
-
-
Huynh, M.H.1
Carruthers, V.B.2
-
68
-
-
28844434092
-
Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum
-
Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T., Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates III J.R., et al. Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum. J Biol Chem 280 (2005) 40169-40176
-
(2005)
J Biol Chem
, vol.280
, pp. 40169-40176
-
-
Sanders, P.R.1
Gilson, P.R.2
Cantin, G.T.3
Greenbaum, D.C.4
Nebl, T.5
Carucci, D.J.6
McConville, M.J.7
Schofield, L.8
Hodder, A.N.9
Yates III, J.R.10
-
69
-
-
4644275337
-
Exogenous control of mammalian gene expression through modulation of RNA self-cleavage
-
Yen L., Svendsen J., Lee J.S., Gray J.T., Magnier M., Baba T., D'Amato R.J., and Mulligan R.C. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature 431 (2004) 471-476
-
(2004)
Nature
, vol.431
, pp. 471-476
-
-
Yen, L.1
Svendsen, J.2
Lee, J.S.3
Gray, J.T.4
Magnier, M.5
Baba, T.6
D'Amato, R.J.7
Mulligan, R.C.8
-
70
-
-
33748469505
-
Exogenous control of mammalian gene expression via modulation of translational termination
-
Murphy G.J., Mostoslavsky G., Kotton D.N., and Mulligan R.C. Exogenous control of mammalian gene expression via modulation of translational termination. Nat Med 12 (2006) 1093-1099
-
(2006)
Nat Med
, vol.12
, pp. 1093-1099
-
-
Murphy, G.J.1
Mostoslavsky, G.2
Kotton, D.N.3
Mulligan, R.C.4
-
71
-
-
33748195107
-
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules
-
Banaszynski L.A., Chen L.C., Maynard-Smith L.A., Ooi A.G., and Wandless T.J. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 126 (2006) 995-1004
-
(2006)
Cell
, vol.126
, pp. 995-1004
-
-
Banaszynski, L.A.1
Chen, L.C.2
Maynard-Smith, L.A.3
Ooi, A.G.4
Wandless, T.J.5
|