Use of Toxoplasma gondii expressing β-galactosidase for colorimetric assessment of drug activity in vitro

Antimicrobial Agents and Chemotherapy

Volumn 41, Issue 9, 1997, Pages 1849-1853

  McFadden, Diane C ^a   Seeber, Frank ^a,b   Boothroyd, John C ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIPROTOZOAL AGENT; ATOVAQUONE; BETA GALACTOSIDASE; CLINDAMYCIN; PYRIMETHAMINE; REAGENT; SULFADIAZINE; URACIL;

ARTICLE; COLORIMETRY; CONTROLLED STUDY; DRUG ACTIVITY; DRUG EFFICACY; ENZYME SUBSTRATE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; TOXOPLASMA GONDII;

EID: 0030826746     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.41.9.1849     Document Type: Article

References (27)

1. Alcina, A., and M. Fresno. 1987. A colorimetric assay based on cell viability of intracellular replication and killing. J. Immunol. Methods 105:1-8.
2. Berg, K., L. Zhai, M. Chen, A. Kharazmi, and T. C. Owen. 1994. The use of a water-soluble formazan complex and mitochondrial function of Leishmania. Parasitol. Res. 80:235-239.
3. Bodley, A. L., M. W. McGarry, and T. A. Shapiro. 1995. Drug cytotoxicity assay for African trypanosomes and Leishmania species. J. Infect. Dis. 172: 1157-1159.
4. Buckner, F. S., C. L. M. J. Verlinde, A. C. La Flamme, and W. C. Van Voorhis. 1996. Efficient technique for screening drugs for activity against Trypanosoma cruzi using parasites expressing β-galactosidase. Antimicrob. Agents Chemother. 40:2592-2597.
5. Bunetel, L., J. Guerin, P. Andre, R. Robert, and J. Deunff. 1995. Calibration of an in vitro assay system using a non-adherent cell line to evaluate the effect of a drug on Toxoplasma gondii. Int. J. Parasitol. 25:699-704.
6. Chio, L. C., and S. F. Queener. 1993. Identification of highly potent and selective inhibitors of Toxoplasma gondii dihydrofolate reductase. Antimicrob. Agents Chemother. 37:1914-1923.
7. Dannemann, B., J. A. McCutchan, D. Israelski, D. Antoniskis, C. Leport, B. Luft, J. Nussbaum, N. Clumeck, P. Morlat, J. Chiu, J.-L. Vilde, M. Orellana, D. Feigal, A. Bartok, P. Heseltine, J. Leedom, J. Remington, and California Collaborative Treatment Group. 1992. Treatment of toxoplasmic encephalitis in patients with AIDS. A randomized trial comparing pyrimethamine plus clindamycin to pyrimethamine plus sulfadiazine. Ann. Inter. Med. 116: 33-43.
8. Derouin, F., and C. Chastang. 1988. Enzyme immunoassay to assess effect of antimicrobial agents on Toxoplasma gondii in tissue culture. Antimicrob. Agents Chemother. 32:303-307.
9. Eyles, D. E., and N. Coleman. 1955. An evaluation of the curative effects of pyrimethamine and sulfadiazine, alone and in combination, on experimental mouse toxoplasmosis. Antibiot. Chemother. 5:529-539.
10. Fichera, M. E., M. K. Bhopale, and D. S. Roos. 1995. In vitro assays elucidate peculiar kinetics of clindamycin action against Toxoplasma gondii. Antimicrob. Agents Chemother. 39:1530-1537.
11. Georgiev, V. S. 1994. Management of toxoplasmosis. Drugs 48:179-188.
12. Hammond, D. J., J. R. Burchell, and M. Pudney. 1985. Inhibition of pyrimidine biosynthesis de novo in Plasmodium falciparum by 2-(4-t-butylcyclohexyl)-3-hydroxy-1,4-naphthoquinone in vitro. Mol. Biochem. Parasitol. 14: 97-109.
13. Iltzsch, M. H., S. S. Uber, K. O. Tankersley, and M. H. el Kouni. 1995. Structure-activity relationship for the binding of nucleoside ligands to adenosine kinase from Toxoplasma gondii. Biochem. Pharmacol. 49:1501-1512.
14. Kim, K., D. Soldati, and J. C. Boothroyd. 1993. Gene replacement in Toxoplasma gondii with chloramphenicol acetyltransferase as selectable marker. Science 262:911-914.
15. Luft, B. J., and J. S. Remington. 1992. Toxoplasmic encephalitis in AIDS. Clin. Infect. Dis. 15:211-222.
16. Mack, D. G., and R. McLeod. 1984. New micromethod to study the effect of antimicrobial agents on Toxoplasma gondii: comparison of sulfadoxine and sulfadiazine individually and in combination with pyrimethamine and study of clindamycin, metronidazole, and cyclosporin A. Antimicrob. Agents Chemother. 26:26-30.
17. Makler, M. T., J. M. Ries, J. A. Williams, J. E. Bancroft, R. C. Piper, B. L. Gibbins, and D. J. Hinrichs. 1993. Parasite lactate dehydrogenase as an assay for Plasmodium falciparum drug sensitivity. Am. J. Trop. Med. Hyg. 48:739-741.
18. Pfefferkorn, E. R., and S. E. Borotz. 1994. Comparison of mutants of Toxoplasma gondii selected for resistance to azithromycin, spiramycin, or clindamycin. Antimicrob. Agents Chemother. 38:31-37.
19. Pfefferkorn, E. R., R. F. Nothnagel, and S. E. Borotz. 1992. Parasiticidal effect of clindamycin on Toxoplasma gondii grown in cultured cells and selection of a drug-resistant mutant. Antimicrob. Agents Chemother. 36: 1091-1096.
20. Pfefferkorn, E. R., and L. C. Pfefferkorn. 1976. Toxoplasma gondii: isolation and preliminary characterization of temperature-sensitive mutants. Exp. Parasitol. 39:365-376.
21. Pfefferkorn, E. R., and L. C. Pfefferkorn. 1977. Specific labeling of intracellular Toxoplasma gondii with uracil. J. Protozool. 24:449-453.
22. Roos, D. S., R. G. K. Donald, N. S. Morrissette, and A. L. C. Moulton. 1994. Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods Cell Biol. 45:27-63.
23. Schoondermark-van de Ven, E., T. Vree, W. Melchers, W. Camps, and J. Galama. 1995. In vitro effects of sulfadiazine and its metabolites alone and in combination with pyrimethamine on Toxoplasma gondii. Antimicrob Agents Chemother. 39:763-765.
24. Seeber, F., and J. C. Boothroyd. 1996. Escherichia coli beta-galactosidase as an in vitro and in vivo reporter enzyme and stable transfection marker in the intracellular protozoan parasite Toxoplasma gondii. Gene 169:39-45.
25. Strair, R. K., D. J. Medina, C. J. Nelson, T. A. Graubert, and J. W. Mellors. 1993. Recombinant retroviral systems for the analysis of drug resistant HIV. Nucleic Acids Res. 21:4836-4842.
26. Tebas, P., E. C. Stabell, and P. D. Olivo. 1995. Antiviral susceptibility testing with cell line which expresses a beta-galactosidase after infection with herpes simplex virus. Antimicrob. Agents Chemother. 39:1287-1291.
27. Varshavsky, A. 1992. The N-end rule. Cell 69:725-735.