Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography

Journal of Chromatography A

Volumn 1082, Issue 2, 2005, Pages 176-184

  Trindade, Inês P ^a   Diogo, Maria M ^b   Prazeres, Duarte M F ^b   Marcos, João C ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Ammonium sulphate; Aqueous two phase systems; DNA; Gene therapy; Liquid liquid extraction; Plasmid; Polyethylene glycol; Purification

Indexed keywords

ALCOHOLS; EXTRACTION; HYDROPHOBICITY; PRECIPITATION (CHEMICAL); PURIFICATION;

AQUEOUS TWO-PHASE SYSTEM (ATPS); DNA VECTORS; ISO-PROPANOL; NEUTRALIZED ALKALINE LYSATES;

DNA;

2 PROPANOL; AMMONIUM SULFATE; MACROGOL; PLASMID DNA;

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; CHROMATOGRAPHY; CONCENTRATION (PARAMETERS); DNA PURIFICATION; DNA VECTOR; EXTRACTION; MOLECULAR MODEL; MOLECULAR WEIGHT; PHASE TRANSITION; PRECIPITATION; PRIORITY JOURNAL; SCREENING;

CHROMATOGRAPHY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; GENETIC VECTORS; PLASMIDS; POLYETHYLENE GLYCOLS;

EID: 20544442647     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2005.05.079     Document Type: Article

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