Engineering the enantioselectivity of glutathione transferase by combined active-site mutations and chemical modifications

Biochimica et Biophysica Acta - General Subjects

Volumn 1770, Issue 9, 2007, Pages 1374-1381

  Ivarsson, Ylva ^a   Norrgård, Malena A ^a   Hellman, Ulf ^a   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Enantioselectivity; Epoxide resolution; Glutathione transferase; Protein modification; Rational redesign

Indexed keywords

CYSTEINE; EPOXIDE; GLUTATHIONE TRANSFERASE; RECOMBINANT ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL MODIFICATION; COMBINATORIAL CHEMISTRY; CONJUGATION; CRYSTAL STRUCTURE; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; MUTATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; STEREOCHEMISTRY;

ALKENES; ALKYLATION; AMINO ACID SEQUENCE; BINDING SITES; CYSTEINE; EPOXY COMPOUNDS; GLUTATHIONE TRANSFERASE; HUMANS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STEREOISOMERISM; STILBENES;

EID: 34547844239     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2007.06.002     Document Type: Article

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