Toward tailoring the specificity of the S1 pocket of subtilisin B. lentus: Chemical modification of mutant enzymes as a strategy for removing specificity limitations

Biochemistry

Volumn 38, Issue 40, 1999, Pages 13391-13397

  DeSantis, Grace ^a   Shang, Xiao ^a   Jones, J B ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE; SUBTILISIN; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CHEMICAL MODIFICATION; EGGERTHELLA LENTA; ENZYME ANALYSIS; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; BACILLUS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; MASS SPECTROMETRY; REPRODUCIBILITY;

BACTERIA (MICROORGANISMS); EGGERTHELLA LENTA; FELIS CATUS;

BACILLUS; BACTERIAL PROTEINS; KINETICS; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SUBSTRATE SPECIFICITY; SUBTILISINS;

EID: 0032589512     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi990861o     Document Type: Article

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