Generation of human scFvs antibodies recognizing a prion protein epitope expressed on the surface of human lymphoblastoid cells

BMC Biotechnology

Volumn 7, Issue , 2007, Pages

  Flego, Michela ^a   Ascione, Alessandro ^a   Zamboni, Silvia ^a   Dupuis, Maria L ^a   Imperiale, Valentina ^a   Cianfriglia, Maurizio ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

FLOW-CYTOMETRY; LYMPHOBLASTOID CELLS;

ANTIBODIES; BIOCHEMISTRY; DISEASES; GENE ENCODING; GENETIC ENGINEERING; PROTEINS;

BIOMEDICAL ENGINEERING;

EPITOPE; ETH 2 ANTIBODY; PRION PROTEIN; PROTEIN ANTIBODY; REAGENT; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; MONOCLONAL ANTIBODY;

ACUTE LYMPHOBLASTIC LEUKEMIA; ANTIGEN RECOGNITION; ARTICLE; BIOCHEMISTRY; BONE MARROW CELL; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GENE SEQUENCE; GENETIC CODE; HAMSTER; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; LEUKEMIA CELL; LYMPHOBLASTOID CELL; MOLECULAR CLONING; NONHUMAN; PRION DISEASE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; ACUTE LYMPHOCYTIC LEUKEMIA; ANTIBODY SPECIFICITY; EPITOPE MAPPING; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; LEUKEMIA CELL LINE; PRION; TUMOR CELL LINE;

CRICETINAE;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CELL LINE, TUMOR; EPITOPE MAPPING; EPITOPES, T-LYMPHOCYTE; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; JURKAT CELLS; LEUKEMIA, LYMPHOCYTIC, ACUTE; PRIONS;

EID: 34547667018     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-7-38     Document Type: Article

References (22)

1. Prusiner SB Prions Proc Natl Acad Sci USA 1998 95 13363-13383 33918 9811807 10.1073/pnas.95.23.13363
2. Pocchiari M Prions and related neurological diseases Mol Aspects Med 1994 15 195-291 10.1016/0098-2997(94)90042-6 7968312
3. Flechsig E Weissmann C The role of PrP in health and disease Curr Mol Med 2004 4 337-53 10.2174/1566524043360645 15354865
4. Weissmann C Enari M Klohn PC Rossi D Flechsig E Trasmission of prions J Infect Dis 2002 186 S157-S165 10.1086/344575 12424692
5. Peretz D Williamson RA Kaneko K Vergara J Leclerc E Schmitt-Ulms G Mehhorn IR Legname G Wormald MR Rudd PM Dwek RA Burton DR Prusiner SB Antibodies inhibit prion propagation and clear cell cultures of prion infectivity Nature 2001 412 739-743 10.1038/35089090 11507642
6. White AR Enever P Tayebl M Mushens R Linehan J Brandner S Anstee D Collinge J Hawke S Monoclonal antibodies inhibit prion replication and delay the development of prion desease Nature 2003 422 80-83 10.1038/ nature01457 12621436
7. Gonzales NR De Pascalis R Schlom J Kashmiri SV Minimizing the immunogenicity of antibodies for clinical application Tumour Biol 2005 26 31-43 10.1159/000084184 15741769
8. Solforosi L Criado JR McGavern DB Wirz S Sánchez-Alavez M Sugama S De Giorgio LA Volpe BT Wiseman E Abalos G Masliah E Gilden D Oldstone MB Conti B Williamson RA Cross-Linking Cellular Prion Protein Triggers Neuronal Apoptosis in Vivo Science 2004 303 1514-1516 10.1126/ science.1094273 14752167
9. Cardinale A Filesi I Vetrugno V Pocchiari M Sy MS Biocca S Trapping prion protein in the endoplasmic reticulum impairs PrPC maturation and prevents PrPSc accumulation J Biol Chem 2005 280 685-94 15513919
10. Ascione A Flego M Zamboni S De Cinti E Dupuis ML Cianfruglia M Application of a synthetic phage antibody library (ETH-2) for the Isolation of single chain fragment variable (scFv) human antibodies to the pathogenic isoform of the hamster prion protein (HaPrPsc) Hybridoma 2005 24 127-132 10.1089/hyb.2005.24.127 15943559
11. Carter P Potent antibody therapeutics by design Nature 2006 6 343-357
12. Ascione A Flego M Zamboni S De Cinti E Dupuis ML Cianfriglia M Isolation and characterization of the human monoclonal antibodies C10 in single-chain fragment variable (scFv) format to glucose oxidase from Aspergillus niger Hybrid Hybridomics 2004 23 380-384 15684666
13. Dodelet VC Cashman NR Prion Protein expression in Human leukocyte differentiation Blood 1998 91 5 1556-1561 9473220
14. Li R Liu D Zanusso G Liu T Fayen JD Huang JH Petersen RB Gambetti P Sy MS The expression and potential function of cellular prion protein in human lymphocytes Cell Immunol 2001 207 49-58 10.1006/cimm.2000.1751 11161453
15. Pan T Li R Wong BS Liu T Gambetti P Sy MS Heterogeneity of normal prion protein in two- dimensional immunoblot: Presence of various glycosylated and truncated form J Neurochem 2002 81 1092-1101 10.1046/ j.1471-4159.2002.00909.x 12065622
16. Beringue V Mallinson G Kaisar M Tayebi M Sattar Z Jackson G Anstee D Collinge J Hawke S Regional heterogeneity of cellular prion protein isoforms in the mouse brain Brain 2003 126 2065-73 10.1093/brain/awg205 12821516
17. Leclerc E Liemann S Wildegger G Vetter SW Nilsson F Selection and characterization of single chain Fv fragments against murine recombinant prion protein from a synthetic human antibody phage display library Hum Antibodies 2000 9 207-14 11341174
18. Enari M Flechsig E Weissmann C Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody Proc Natl Acad Sci USA 2001 98 9295-9299 55414 11470893 10.1073/pnas.151242598
19. Heppner FL Musahl C Arrighi I Klein MA Rulicke T Oesch B Zinkernagel RM Kalinke U Aguzzi A Prevention of scrapie pathogenesis by transgenic expression of anti-prion protein antibodies Science 2001 294 178-182 10.1126/science.1063093 11546838
20. Bade S Frey A Potential of active and passive immunizations for the prevention and therapy of transmissible spongiform encephalopathies Expert Rev Vaccines 2007 6 153-168 10.1586/14760584.6.2.153 17408366
21. Kohler G Milstein C Continuous cultures of fused cells secreting antibody of predefined specificity J Immunol 1975 256 5517 495-497
22. Viti F Nilsson F Demartis S Huber A Neri D Design and use of phage display libraries for the selection of antibodies and enzymes Methods Enzymol 2000 326 480-505 11036659