Application of a synthetic phage antibody library (ETH-2) for the isolation of single chain fragment variable (scFv) human antibodies to the pathogenic isoform of the hamster prion protein (HaPrPsc)

Hybridoma

Volumn 24, Issue 3, 2005, Pages 127-132

  Ascione, Alessandro ^a   Flego, Michela ^a   Zamboni, Silvia ^a   De Cinti, Emanuela ^a   Dupuis, Maria Luisa ^a   Cianfriglia, Maurizio ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; MONOCLONAL ANTIBODY; PRION PROTEIN; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ANTIGEN BINDING; ANTIGEN RECOGNITION; ARTICLE; BACTERIAL GENE; BACTERIOPHAGE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE AMPLIFICATION; GENE SEQUENCE; HAMSTER; HYBRIDOMA; IMMUNOGENICITY; IN VITRO STUDY; LYMPHOID CELL; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

CRICETINAE;

EID: 20844459340     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2005.24.127     Document Type: Article

References (19)

1. Prusiner SB: Prions. Proc Natl Acad Sci USA 1998;95: 13363-13383.
2. Pocchiari M: Prions and related neurological diseases. Mol Aspects Med 1994;15:195-291.
3. Meyer RK, McKinley MP, Bowman KA, Braunfeld MB, Barry RA, and Prusiner SB: Separation and properties of cellular and scrapie prion protein. Proc Natl Acad Sci USA 1986;83: 2310-2314.
4. Weissmann C: Molecular genetics of transmissible spongiform encephalopathies. J Biol Chem 1999;274:3-6.
5. Weissmann C, Enari M, Klohn PC, Rossi D, and Flechsig E: Transmission of prions. J Infect Dis 2002;186:S157-S165.
6. Peretz D, Williamson RA, Kaneko K, Vergara J, Ledere E, Schmitt-Ulms G, Mehhorn IR, Legname G, Wormald MR, Rudd PM, Dwek RA, Burton DR, and Prusiner SB: Antibodies inhibit prion propagation and clear cell cultures of prion infectivity. Nature 2001;412:739-743.
7. White AR, Enever P, Tayebl M, Mushens R, Linehan J, Brandner S, Anstee D, Collinge J, and Hawke S: Monoclonal antibodies inhibit prion replication and delay the development of prion disease. Nature 2003;422:80-83.
8. Kascsack RJ, Rubenstein R, Merz PA, Tonna-DeMasi M, Fersko R, Carp RI, Wisniewski HM, and Diringer H: Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J Virol 1987;61:3688-3693.
9. Nakamura N, Miyamoto K, Shimokawa M, Nishida N, Mohri S, Kitamoto T, Horiuchi H, Furusawa S, and Matsuda H: Generation of antibodies against prion protein by scrapie-infected cell immunization of PrP (0/0) mice. Hybrid Hybridomics 2003;22:263-266.
10. Kim C-L, Umetani A, Matsui T, Ishiguro N, Shinagawa M, and Horiuchi M: Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies. Virology 2004;320:40-51.
11. Silvestrini MC, Cardone F, Maras B, Pucci P, Barra D, Brunori M, and Pocchiari M: Identification of the prion protein allotypes which accumulate in the brain of sporadic and familial Creutzfeldt-Jakob disease patients. Nat Med 1997;3:521-525.
12. Viti F, Nilsson F, Demartis S, Huber A, and Neri D: Design and use of phage display libraries for the selection of antibodies and enzyme. Methods Enzymol 2000;326:480-505.
13. Barry RA, and Prusiner SB: Monoclonal antibodies to the cellular and scrapie prion protein. J Infect Dis 1986;154:518-521.
14. Bodemer W: The use of monoclonal antibodies in human prion disease. Naturwissenschaften 1999;86:212-220.
15. Rogers M, Serban D, Gyuris T, Scott M, Torchia T, and Prusiner SB: Epitope mapping of the Syrian hamster prion protein utilizing chimeric and mutant genes in vaccinia virus expression system. J Immunol 1991;147:3568-3574.
16. MacGregor I: Prion protein and developments in its detection. Transfus Med 2001;11:3-14.
17. Williamson RA, Peretz D, Pinilla C, Ball H, Bastidas RB, Rozenshteyn R, Prusiner SB, and Burton DR: Mapping the prion protein using recombinam antibodies. J Virol 1998;72:9413-9418.
18. Li R, Liu D, Zanusso G, Liu T, Fayen JD, Huang JH, Petersen RB, Gambetti P, and Sy MS: The expression and potential function of cellular prion protein in human lymphocytes. Cell Immunol 2001;207:49-58.
19. Cashman NR, Loertscher R, Nalbantoglu J, Shaw I, Kascsak RJ, Bolton DC, and Bendheim PE: Cellular isoform of the scrapie agent protein participates in lymphocyte activation. Cell 1990;61: 185-192.