Biochemical and X-ray crystallographic studies on shikimate kinase: The important structural role of the P-loop lysine

Protein Science

Volumn 10, Issue 6, 2001, Pages 1137-1149

  Krell, Tino ^a,e   Maclean, John ^b,f   Boam, Deborah J ^a   Cooper, Alan ^b   Resmini, Marina ^c   Brocklehurst, Keith ^c   Kelly, Sharon M ^d   Price, Nicholas C ^d   Lapthorn, Adrian J ^b   Coggins, John R ^a,b  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^d UNIVERSITY OF STIRLING   (United Kingdom)

^e SANOFI PASTEUR   (France)

^f PanTherix Ltd   (United Kingdom)

Author keywords

Enzyme stability; Ion pair; P loop lysine; Shikimate kinase

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENYLATE KINASE; ARGININE ASPARTATE; CYSTEINE; LYSINE; MUTANT PROTEIN; NUCLEOSIDE MONOPHOSPHATE KINASE; NUCLEOTIDE; SHIKIMATE KINASE; SHIKIMIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; ION PAIR EXTRACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY;

2,2'-DIPYRIDYL; ADENOSINE TRIPHOSPHATE; ARGININE; ASPARTIC ACID; BINDING SITES; CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DISULFIDES; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; LYSINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXIDANTS; PECTOBACTERIUM CHRYSANTHEMI; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN BINDING; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; SULFHYDRYL REAGENTS; TEMPERATURE; TRINITROBENZENESULFONIC ACID; ULTRAVIOLET RAYS;

EID: 0035008584     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.52501     Document Type: Article

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