Purine nucleoside phosphorylase from Cellulomonas sp.: Physicochemical properties and binding of substrates determined by ligand-dependent enhancement of enzyme intrinsic fluorescence, and by protective effects of ligands on thermal inactivation of the enzyme

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1597, Issue 2, 2002, Pages 320-334

  Wielgus Kutrowska, Beata ^a   Bzowska, Agnieszka ^a   Tebbe, Jan ^b   Koellner, Gertraud ^b   Shugar, David ^a,c  

^a UNIVERSITY OF WARSAW   (Poland)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

Cellulomonas sp.; Guanine anion; Mechanism of catalysis; Protein fluorescence; Protein ligand interaction; Purine nucleoside phosphorylase

Indexed keywords

GUANINE; GUANOSINE; INOSINE; LIGAND; PURINE NUCLEOSIDE PHOSPHORYLASE; TRYPTOPHAN;

ARTICLE; CATALYSIS; CATALYST; CELLULOMONAS; CONCENTRATION RESPONSE; CONFORMATION; CONTROLLED STUDY; DISSOCIATION; FLUORESCENCE; IONIC STRENGTH; KINETICS; LIGAND BINDING; MICROENVIRONMENT; MOLECULAR WEIGHT; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN BINDING; STOICHIOMETRY; THERMOSTABILITY; VELOCITY;

ACTINOMYCETALES; CATALYSIS; CHEMISTRY, PHYSICAL; GUANOSINE; HEAT; HYDROGEN-ION CONCENTRATION; HYPOXANTHINE; KINETICS; LIGANDS; MOLECULAR WEIGHT; PHOSPHATES; PURINE-NUCLEOSIDE PHOSPHORYLASE; RIBOSEMONOPHOSPHATES; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET; SUBSTRATE SPECIFICITY;

CELLULOMONAS; CELLULOMONAS SP.;

EID: 0037014054     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(02)00313-8     Document Type: Article

References (60)

1. Purine nucleoside phosphorylase: Properties, functions and clinical aspects
2. Nucleoside-phosphorylase deficiency in a child with severely defective T-cell immunity and normal B-cell immunity
3. Purine nucleoside phosphorylase: A target for chemotherapy
4. Tumour bystander killing in colonic carcinoma utilizing the Escherichia coli DeoD gene to generate toxic purines
5. Bystander killing of melanoma cells using the human tyrosinase promoter to express the Escherichia coli purine nucleoside phosphorylase gene
6. Cell to cell contact is not required for bystander cell killing by Escherichia coli purine nucleoside phosphorylase
7. Thymidine kinase-deleted vaccinia virus expressing purine nucleoside phosphorylase as a vector for tumour-directed gene therapy
8. Expression of human malaria parasite purine nucleoside phosphorylase in host enzyme-deficient erythrocyte culture
9. Trypanosoma cruzi adenine nucleoside phosphorylase
10. Purine nucleoside synthesis, an efficient method employing nucleoside phosphorylase
11. Novel 6-alkoxypurine 2′,3′-dideoxy nucleosides as inhibitors of the cytopathic effects of the human immunodeficiency virus
12. A new method for the synthesis of nucleosides using purine nucleoside phosphorylase
13. Facile synthesis of 5′-deoxy- and 2′,5′-dideoxy-6-thiopurine nucleosides by nucleoside phosphorylases
14. Mechanism of 2′,3′-dideoxyadenosine synthesis by Escherichia coli AJ 2595
15. Synthesis of (2′S,3′S)-9-(4′-phosphono-4′,4′-difluoro- 2′,3′-methanobutyl)guanine and its enantiomer: Evaluation of the inhibitory activity of purine nucleoside phosphorylase
16. Synthesis of 1,1-difluoro-5-(1H-9-purinyl)-2-pentenphosphonic acids and the related methano analogues: Remarkable effect of the nucleobases and the cyclopropane ring on inhibitory activity toward purine nucleoside phosphorylase
17. Synthesis and biological evaluation of 1,1-difluoro-2-(tetrahydro-3-furanyl)ethylphosphonic acids possessing an N9-purinylmethyl functional group at the ring: A new class of inhibitor for purine nucleoside phosphorylase
18. Crystal structures of purine nucleoside phosphorylase (PNP) from Cellulomonas sp. and its implications for the molecular mechanism of trimeric PNPs
19. Cellulomonas sp. purine nucleoside phosphorylase: Comparison with human and E. coli enzymes
20. Crystallization and preliminary X-ray studies of purine nucleoside phosphorylase from Cellulomonas sp
21. Purine nucleoside phosphorylase (PNP) from Cellulomonas sp., a third class of PNP different from both "low-molecular weight" mammalian and "high-molecular weight" bacterial PNPs
22. Binding of substrates by purine nucleoside phosphorylase (PNP) from Cellulomonas sp. - Kinetic and spectrofluorimetric studies
23. Assay for inorganic phosphate, total phosphate and phosphonates
24. 7-Deazapurine 2′-deoxyribofuranosides are non-cleavable competitive inhibitors of Escherichia coli purine nucleoside phosphorylase
25. Differential spectrophotometry of purine compounds by means of specific enzymes: I. Determination of hydroxypurines
26. Purine nucleoside phosphorylase from human erythrocytes
27. Protein measurement with the Folin phenol reagent
28. Quantitation of proteins
29. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
30. Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol
31. Calf spleen purine nucleoside phosphorylase: Purification, sequence and crystal structure of its complex with an N(7)-acycloguanosine inhibitor
32. Spectral data and pK values for purines, pyrimidines, nucleosides, and nucleotides
33. Misuse of nonlinear Scatchard plots
34. 'Open' and 'closed' conformation of the E. coli purine nucleoside phosphorylase active center and implications for the catalytic mechanism
35. 2+ binding
36. Purine nucleoside phosphorylase: Its use in a spectroscopic assay for inorganic phosphate and for removing inorganic phosphate with the aid of phosphodeoxyribomutase
37. Structure and function of mutationally generated monomers of dimeric phosphoribosylanthranilate isomerase from Themotoga martima
38. Purine nucleoside phosphorylase: Kinetic mechanism for the enzyme from calf spleen
39. Bovine brain purine nucleoside phosphorylase: Purification, characterization, and catalytic mechanism
40. Purification and properties of inosine-guanosine phosphorylase form Escherichia coli K-12
41. Enzymes of the human erythrocyte: Purine nucleoside phosphorylase, specific properties
42. Purine nucleoside phosphorylase
43. b transitions of tryptophane; Applications of theory and experimental observations to fluorescence of proteins
44. Fluorescence methods for studying equilibrium macromolecule-ligand interactions
45. Purine nucleoside phosphorylase: Inosine hydrolysis, tight binding of the hypoxanthine intermediate, and third-the-sites reactivity
46. Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues
47. Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 Å resolution
48. Fluorescence properties of alkylated guanine derivatives
49. Ultraviolet and fluorescence characterization of purines and pyrimidines by post-column pH manipulation
50. Effects of cooling on the fluorescence of biological guanine derivatives
51. Tautomerism of purines and pyrimidines, their nucleosides and various analogues
52. Transition state structure of purine nucleoside phosphorylase and principles of atomic motion in enzymatic catalysis
53. Purine nucleoside phosphorylase: 2. Catalytic mechanism
54. Crystallization and some properties of purine nucleoside phosphorylase from chicken liver
55. Purine nucleoside phosphorylase from human erythrocytes: A kinetic study of the fully separated isoenzymes
56. Purine nucleoside phosphorylase: Allosteric regulation of a dissociating enzyme
57. Allosteric regulation of purine nucleoside phosphorylase
58. Calf spleen purine nucleoside phosphorylase: Complex kinetic mechanism, hydrolysis of 7-methylguanosine, and oligomeric state in solution