Antisense PNA accumulates in Escherichia coli and mediates a long post-antibiotic effect

Molecular Therapy

Volumn 15, Issue 8, 2007, Pages 1537-1542

  Nikravesh, Abbas ^a,b   Dryselius, Rikard ^a,c   Faridani, Omid R ^a   Goh, Shan ^a   Sadeghizadeh, Majid ^b   Behmanesh, Mehrdad ^b   Ganyu, Anita ^a   Klok, Erik Jan ^d   Zain, Rula ^e   Good, Liam ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b TARBIAT MODARES UNIVERSITY   (Iran)

^c OSAKA UNIVERSITY   (Japan)

^d Kreatech Biotechnology BV   (Netherlands)

^e STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEIN; AMPICILLIN; BETA GALACTOSIDASE; CHLORAMPHENICOL; MESSENGER RNA; PEPTIDE NUCLEIC ACID; RIFAMPICIN; STREPTOMYCIN; TRIMETHOPRIM;

ANTIBACTERIAL ACTIVITY; ANTISENSE THERAPY; ARTICLE; BIOACCUMULATION; CELL KILLING; CONTROLLED STUDY; DRUG MECHANISM; DRUG UPTAKE; ESCHERICHIA COLI; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POSTANTIBIOTIC EFFECT;

ACYL CARRIER PROTEIN; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; DIPEPTIDES; DNA, ANTISENSE; ESCHERICHIA COLI; KINETICS; MICROBIAL VIABILITY; PEPTIDE NUCLEIC ACIDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MUS;

EID: 34547136933     PISSN: 15250016     EISSN: 15250024     Source Type: Journal    
DOI: 10.1038/sj.mt.6300209     Document Type: Article

References (27)

1. Keren, I, Shah, D, Spoering, A, Kaldalu, N and Lewis, K (2004). Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli. J Bacteriol 186: 8172-8180.
2. Wagner, EG, Altuvia, S and Romby, P (2002). Antisense RNAs in bacteria and their genetic elements. Adv Genet 46: 361-398.
3. Nielsen, PE, Egholm, M, Berg, RH and Buchardt, O (1991). Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science 254: 1497-1500.
4. Stirchak, EP, Summerton, JE and Weller, DD (1989). Uncharged stereoregular nucleic acid analogs: 2. Morpholino nucleoside oligomers with carbamate internucleoside linkages. Nucleic Acids Res 17 6129-6141.
5. Lehtola, MJ, Loades, CJ and Keevil, CW (2005). Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari. J Microbiol Methods 62: 211-219.
6. Prescott, AM and Fricker, CR (1999). Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water. Mol Cell Probes 13: 261-268.
7. Good, L, Awasthi, SK, Dryselius, R, Larsson, O and Nielsen, PE (2001). Bactericidal antisense effects of peptide-PNA conjugates. Nat Biotechnol 19: 360-364.
8. Eriksson, M, Nielsen, PE and Good, L (2002). Cell permeabilization and uptake of antisense peptide-peptide nucleic acid (PNA) into Escherichia coli. J Biol Chem 277: 7144-7147.
9. Tan, XX, Actor, JK and Chen, Y (2005). Peptide nucleic acid antisense oligomer as a therapeutic strategy against bacterial infection: Proof of principle using mouse intraperitoneal infection. Antimicrob Agents Chemother 49: 3203-3207.
10. Faridani, OR, Nikravesh, A, Pandey, DP, Gerdes, K and Good, L (2006). Competitive inhibition of natural antisense Sok-RNA interactions activates Hok-mediated cell killing in Escherichia coli. Nucleic Acids Res 34: 5915-5922.
11. Geller, BL, Deere, J, Tilley, L and Iversen, PL (2005). Antisense phosphorodiamidate morpholino oligomer inhibits viability of Escherichia coli in pure culture and in mouse peritonitis. J Antimicrob Chemother 55: 983-988.
12. Geller, BL, Deere, JD, Stein, DA, Kroeker, AD, Moulton, HM and Iversen, PL (2003). Inhibition of gene expression in Escherichia coli by antisense phosphorodiamidate morpholino oligomers. Antimicrob Agents Chemother 47: 3233-3239.
13. Tilley, LD, Mellbye, BL, Puckett, SE, Iversen, PL and Geller, BL (2007). Antisense peptide-phosphorodiamidate morpholino oligomer conjugate: dose-response in mice infected with Escherichia coli. J Antimicrob Chemother 59: 66-73.
14. Gruegelsiepe, H, Brandt, O and Hartmann, RK (2006). Antisense inhibition of RNase P: Mechanistic aspects and application to live bacteria. J Biol Chem 281: 30613-30620.
15. Nekhotiaeva, N, Awasthi, SK, Nielsen, PE and Good, L (2004). Inhibition of Staphylococcus aureus gene expression and growth using antisense peptide nucleic acids. Mol Ther 10: 652-659.
16. Kurupati, P, Tan, KS, Kumarasinghe, G and Poh, CL (2007). Inhibition of gene expression and growth by antisense peptide nucleic acids in a multiresistant β-lactamase-producing Klebsiella pneumoniae. Antimicrob Agents Chemother 51: 805-811.
17. Dryselius, R, Nikravesh, A, Kulyte, A, Goh, S and Good, L (2006). Variable coordination of cotranscribed genes in Escherichia coli following antisense repression. BMC Microbiol 6: 97.
18. Kulyte, A, Nekhotiaeva, N, Awasthi, SK and Good, L (2005). Inhibition of Mycobacterium smegmatis gene expression and growth using antisense peptide nucleic acids. J Mol Microbiol Biotechnol 9: 101-109.
19. Williams, KJ and Piddock, LJ (1998). Accumulation of rifampicin by Escherichia coli and Staphylococcus aureus. J Antimicrob Chemother 42: 597-603.
20. Lebaron, P, Catala, P and Parthuisot, N (1998). Effectiveness of SYTOX Green stain for bacterial viability assessment. Appl Environ Microbiol 64: 2697-2700.
21. McMahon, BM, Mays, D, Lipsky, J, Stewart, JA, Fauq, A and Richelson, E (2002). Pharmacokinetics and tissue distribution of a peptide nucleic acid after intravenous administration. Antisense Nucleic Acid Drug Dev 12: 65-70.
22. Good, L, Sandberg, R, Larsson, O, Nielsen, PE and Wahlestedt, C (2000). Antisense PNA effects in Escherichia coli are limited by the outer-membrane LPS layer. Microbiology 146: 2665-2670.
23. Saenz, Y, Ruiz, J, Zarazaga, M, Teixido, M, Torres, C and Vila, J (2004). Effect of the efflux pump inhibitor Phe-Arg-beta-naphthylamide on the MIC values of the quinolones, tetracycline and chloramphenicol, in Escherichia coli isolates of different origin. J Antimicrob Chemother 53: 544-545.
24. Stubbings, WJ, Bostock, JM, Ingham, E and Chopra, I (2004). Assessment of a microplate method for determining the post-antibiotic effect in Staphylococcus aureus and Escherichia coli. J Antimicrob Chemother 54: 139-143.
25. George, AM and Hall, RM (2002). Efflux of chloramphenicol by the CmlA1 protein. FEMS Microbiol Lett 209: 209-213.
26. Falagas, ME and Michalopoulos, A (2006). Polymyxins: Old antibiotics are back. Lancet 367: 633-634.
27. Livak, KJ and Schmittgen, TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25: 402-408.