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Volumn 8, Issue 8, 2007, Pages 875-879

Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles

Author keywords

Aggregation; Enzymes; Gold; High throughput screening

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; GOLD; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE ACTIVATED PROTEIN KINASE 2; NANOPARTICLE; PROTEIN KINASE; PROTEIN KINASE (CALCIUM,CALMODULIN) II; PROTEIN KINASE C ALPHA; UNCLASSIFIED DRUG; CELL EXTRACT; METAL NANOPARTICLE;

EID: 34447523592     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200700086     Document Type: Article
Times cited : (74)

References (31)
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    • e) R.-H. Yeh, X. Yan, M. Cammer, A. R. Bresnick, D. S. Lawrence, J. Biol. Chem. 2002, 277, 11 527-11 532. We thank the referee for bringing our attention to these works.
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    • The concentrations of the peptides were optimized to effectively differentiate substrates and phosphorylated peptides Figure S1
    • The concentrations of the peptides were optimized to effectively differentiate substrates and phosphorylated peptides (Figure S1).
  • 25
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    • h much larger.
    • h much larger.
  • 27
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    • 50 values were almost unchanged when the GNPs were used within six months after preparation.
    • 50 values were almost unchanged when the GNPs were used within six months after preparation.
  • 28
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    • We found that the peptide-induced aggregation of GNPs is perturbed when the lysate concentrations exceed 600 μg mL-1. Thus, in the lysate experiments, their concentrations were adjusted to well below that concentration 100 or 300 μg mL-1
    • -1).
  • 30
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    • Hyperactivation of PKCα in B16 and the drug-stimulated activation of PKA in MCF-7 were confirmed by Western blotting analyses (Figure S5).
    • Hyperactivation of PKCα in B16 and the drug-stimulated activation of PKA in MCF-7 were confirmed by Western blotting analyses (Figure S5).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.