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The concentrations of the peptides were optimized to effectively differentiate substrates and phosphorylated peptides Figure S1
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The concentrations of the peptides were optimized to effectively differentiate substrates and phosphorylated peptides (Figure S1).
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25
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34547581077
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h much larger.
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h much larger.
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0034306450
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50 values were almost unchanged when the GNPs were used within six months after preparation.
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50 values were almost unchanged when the GNPs were used within six months after preparation.
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28
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We found that the peptide-induced aggregation of GNPs is perturbed when the lysate concentrations exceed 600 μg mL-1. Thus, in the lysate experiments, their concentrations were adjusted to well below that concentration 100 or 300 μg mL-1
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-1).
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Hyperactivation of PKCα in B16 and the drug-stimulated activation of PKA in MCF-7 were confirmed by Western blotting analyses (Figure S5).
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Hyperactivation of PKCα in B16 and the drug-stimulated activation of PKA in MCF-7 were confirmed by Western blotting analyses (Figure S5).
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