Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids

Journal of Bacteriology

Volumn 189, Issue 14, 2007, Pages 5170-5182

  Hirooka, Kazutake ^a   Kunikane, Satoshi ^a   Matsuoka, Hiroshi ^a   Yoshida, Ken Ichi ^b   Kumamoto, Kanako ^a   Tojo, Shigeo ^a   Fujita, Yasutaro ^a  

^a FUKUYAMA UNIVERSITY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA GALACTOSIDASE; CATECHIN; DEOXYRIBONUCLEASE I; DIOXYGENASE; FISETIN; FLAVONOID; GALANGIN; PROTEIN LMRA; PROTEIN YXAF; QUERCETIN; QUERCETIN 2,3 DIOXYGENASE; TAMARIXETIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CONTROLLED STUDY; DNA BINDING; DNA FOOTPRINTING; ENZYME SYNTHESIS; GEL RETARDATION ANALYSIS; GENE CONTROL; GENE SEQUENCE; IN VITRO STUDY; IN VIVO STUDY; LACZ GENE; LMRAB GENE; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REGULON; REPRESSOR GENE; TECHNIQUE; YXAG GENE; YXAGH GENE;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; CATECHIN; DIOXYGENASES; DISACCHARIDES; DNA FOOTPRINTING; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FLAVONOIDS; GENE EXPRESSION REGULATION, BACTERIAL; LINCOMYCIN; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OPERON; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; QUERCETIN; REGULON; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

BACILLUS SUBTILIS;

EID: 34447511578     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00079-07     Document Type: Article

References (30)

1. Atkinson, M. R., L. V. Wray, Jr., and S. H. Fisher. 1990. Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis. J. Bacteriol. 172:4758-4765.
2. Barney, B. M., M. R. Schaab, R. LoBrutto, and W. A. Francisco. 2004. Evidence for a new metal in a known active site: purification and characterization of an iron-containing quercetin 2,3-dioxygenase from Bacillus subtilis. Protein Expr. Purif. 35:131-141.
3. Bowater, L., S. A. Fairhurst, V. J. Just, and S. Bornemann. 2004. Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase. FEBS Lett. 557:45-48.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Dodd, I. B., and J. B. Egan. 1990. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucleic Acids Res. 18:5019-5026.
6. Fujita, Y., and Y. Miwa. 1989. Identification of an operator sequence for the Bacillus subtilis gnt operon. J. Biol. Chem. 264:4201-4206.
7. Haldenwang, W. G. 1995. The sigma factors of Bacillus subtilis. Microbiol. Rev. 59:1-30.
8. Horinouchi, S., and B. Weisblum. 1982. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J. Bacteriol. 150:815-825.
9. Kobayashi, H., Y. Naciri-Graven, W. J. Broughton, and X. Perret. 2004. Flavonoids induce temporal shifts in gene-expression of nod-box controlled loci in Rhizobium sp. NGR234. Mol. Microbiol. 51:335-347.
10. Kobayashi, K., S. D. Ehrlich, A. Albertini, et al. 2003. Essential Bacillus subtilis genes. Proc. Natl. Acad. Sci. USA 100:4678- 4683.
11. Kobayashi, K., M. Ogura, H. Yamaguchi, K. Yoshida, N. Ogasawara, T. Tanaka, and Y. Fujita. 2001. Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems. J. Bacteriol. 183:7365-737025.
12. Kumano, M., M. Fujita, K. Nakamura, M. Murata, R. Ohki, and K. Yamane. 2003. Lincomycin resistance mutations in two regions immediately downstream of the -10 region of lmr promoter cause overexpression of a putative multidrug efflux pump in Bacillus subtilis mutants. Antimicrob. Agents Chemother. 47:432-435.
13. Kunst, F., N. Ogasawara, I. Moszer, et al. 1997. The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390:249-256.
14. Murata, M., S. Ohno, M. Kumano, K. Yamane, and R. Ohki. 2003. Multidrug resistant phenotype of Bacillus subtilis spontaneous mutants isolated in the presence of puromycin and lincomycin. Can. J. Microbiol. 49:71-77.
15. Ogura, M., H. Yamaguchi, K. Yoshida, Y. Fujita, and T. Tanaka. 2001. DNA microarray analysis of Bacillus subtilis DegU, ComA and PhoP regulons: an approach to comprehensive analysis of B. subtilis two-component regulatory systems. Nucleic Acids Res. 29:3804-3813.
16. Plaper, A., M. Golob, I. Hafner, M. Oblak, T. Solmajer, and R. Jerala. 2003. Characterization of quercetin binding site on DNA gyrase. Biochem. Biophys. Res. Commun. 306:530-536.
17. Ramos, J. L., M. Martínez-Bueno, A. J. Molina-Henares, W. Terán, K. Watanabe, X. Zhang, M. T. Gallegos, R. Brennan, and R. Tobes. 2005. The TetR family of transcriptional repressors. Microbiol. Mol. Biol. Rev. 69:326-356.
18. Rao, J. R., and J. E. Cooper. 1994. Rhizobia catabolize nod gene-inducing flavonoids via C-ring fission mechanisms. J. Bacteriol. 176:5409-5413.
19. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
20. Satomura, T., D. Shimura, K. Asai, Y. Sadaie, K. Hirooka, and Y. Fujita. 2005. Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system. J. Bacteriol. 187:4813-4821.
21. Seetharaman, J., D. Kumaran, J. B. Bonanno, S. K. Burley, and S. Swaminathan. 2006. Crystal structure of a putative HTH-type transcriptional regulator yxaF from Bacillus subtilis. Proteins 63:1087-1091.
22. Steinmetz, M., and R. Richter. 1994. Plasmids designed to alter the antibiotic resistance expressed by insertion mutations in Bacillus subtilis, through in vivo recombination. Gene 142:79-83.
23. Terán, W., T. Krell, J. L. Ramos, and M. T. Gallegos. 2006. Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J. Biol. Chem. 281:7102-7109.
24. Ulanowska, K., A. Tkaczyk, G. Konopa, and G. Wegrzyn. 2006. Differential antibacterial activity of genistein arising from global inhibition of DNA, RNA and protein synthesis in some bacterial strains. Arch. Microbiol. 184:271-278.
25. Vagner, V., E. Dervyn, and S. D. Ehrlich. 1998. A vector for systematic gene inactivation in Bacillus subtilis. Microbiology 144:3097-3104.
26. Wach, A. 1996. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae. Yeast 12:259-265.
27. Yoshida, K., Y. Fujita, and A. Sarai. 1993. Missense mutations in the Bacillus subtilis gnt repressor that diminish operator binding ability. J. Mol. Biol. 231:167-174.
28. Yoshida, K., I. Ishio, E. Nagakawa, Y. Yamamoto, M. Yamamoto, and Y. Fujita. 2000. Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome. Microbiology 146:573-579.
29. Yoshida, K., K. Kobayashi, Y. Miwa, C. M. Kang, M. Matsunaga, H. Yamaguchi, S. Tojo, M. Yamamoto, R. Nishi, N. Ogasawara, T. Nakayama, and Y. Fujita. 2001. Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis. Nucleic Acids Res. 29:683-692.
30. Yoshida, K., Y. Ohki, M. Murata, M. Kinehara, H. Matsuoka, T. Satomura, R. Ohki, M. Kumano, K. Yamane, and Y. Fujita. 2004. Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH. J. Bacteriol. 186:5640-5648.