Purification and in vitro refolding of maize chloroplast transglutaminase over-expressed in Escherichia coli

Biotechnology Letters

Volumn 29, Issue 8, 2007, Pages 1255-1262

  Carvajal Vallejos, Patricia K ^a   Campos, Alexandre ^b   Fuentes Prior, Pablo ^c   Villalobos, Enrique ^d   Almeida, André M ^b   Barberà, Eduard ^a   Torné, José María ^d   Santos, Mireya ^d  

^a INSTITUT QUÍMIC DE SARRIÀ   (Spain)

^b INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^c HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

^d INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

Author keywords

Escherichia coli; Maize chloroplast; Purification; Refolding; Transglutaminase

Indexed keywords

CATALYST ACTIVITY; CHLOROPHYLL; ESCHERICHIA COLI; GENE EXPRESSION; PROTEIN FOLDING; PURIFICATION;

GUANIDINE; MAIZE CHLOROPLAST; REFOLDING; TRANSGLUTAMINASE;

ENZYME ACTIVITY;

GUANIDINE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT PROTEIN; UREA;

ARTICLE; BIOTECHNOLOGY; CHEMISTRY; CHLOROPLAST; ENZYMOLOGY; ESCHERICHIA COLI; MAIZE; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; PROTEIN FOLDING;

BIOTECHNOLOGY; CHLOROPLASTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GUANIDINE; PROTEIN DENATURATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSGLUTAMINASES; UREA; ZEA MAYS;

ESCHERICHIA COLI; MAMMALIA; ZEA MAYS;

EID: 34447135632     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9377-7     Document Type: Article

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