PCR amplification introduces errors into mononucleotide and dinucleotide repeat sequences

Journal of Clinical Pathology - Molecular Pathology

Volumn 54, Issue 5, 2001, Pages 351-353

  Clarke, L A ^a   Rebelo, C S ^a   Goncalves J ^a   Boavida, M G ^a   Jordan, P ^a  

^a NATIONAL INSTITUTE OF HEALTH DR RICARDO JORGE   (Portugal)

Author keywords

Nucleotide repeat sequences; Polymerase chain reaction; Shadow bands

Indexed keywords

DNA;

ANALYTICAL ERROR; ARTICLE; DNA POLYMORPHISM; GENE AMPLIFICATION; GENE MUTATION; GENETIC MARKER; HUMAN; MOLECULAR CLONING; NORMAL HUMAN; NUCLEOTIDE REPEAT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

DINUCLEOTIDE REPEATS; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA;

EID: 0034817160     PISSN: 13668714     EISSN: None     Source Type: Journal    
DOI: 10.1136/mp.54.5.351     Document Type: Article

References (25)

1. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polsmaerases
2. Bat-26, an indicator of the replication error phenotype in colorectal cancers and cell lines
3. Allelic profiles of mononucleotide repeat microsatellites in control individuals and in colorectal turnouts with or without replication errors
4. Small GTPase Rac1: Structure, localisation and expression of the human gene
5. A second-generation linkage map of the human genome
6. Sequence of the intron/exon junctions of the coding region of the human androgen receptor gene and identification of a point mutation in a family with complete androgen insensitivity
7. Molecular genetics of triplet repeats: Unstable expansions of triplet repeats as a new mechanism for neurodegenerative diseases
8. Glutamine repeats and neurodegeneration
9. Sequence analysis and characterization of stutter products of the tetranucleotide repeat locus vWA
10. A study of the origin of "shadow bands" seen when typing dinucleotide repeat polymorphisms by the PCR
11. Shadow bands seen when typing polymorphic dinucleotide repeats: Some causes and cures
12. The determination of the sequences present in the shadow bands of a dinucleotide repeat PCR
13. Origin of spurious multiple bands in the amplification of microsatellite sequences
14. Slipped-strand mispairing: A major mechanism for DNA sequence evolution
15. Gene deletions causing human genetic disease: Mechanisms of mutagenesis and the role of the local DNA sequence environment
16. Automated detection of trinucleotide repeats in fragile X syndrome
17. Enzymatic amplification of synthetic oligodeoxyribonucleotides: Implications for triplet expansions in the human genome
18. Improved polymerase fidelity in PCR-SSCPA
19. 2+ concentration and mismatches on triplet-repeat expansion during DNA replication in vitro
20. Amplification of GAA/TTC triplet repeat in vitro: Preferential expansion of (TTC)n strand
21. The mutational specificity of DNA polymerase-β during in-vitro DNA synthesis
22. Mismatch repair gene defects in sporadic colorectal cancers with microsatellite instability
23. Recognition of DNA alterations by the mismatch repair system
24. National Cancer Institute workshop on hereditary non-polyposis colorectal cancer syndrome: Meeting highlights and Bethesda guidelines
25. Hereditary non-polyposis colorectal cancer (HNPCC)