Electrophysiological studies in Xenopus oocytes for the opening of Escherichia coli SecA-dependent protein-conducting channels

Journal of Membrane Biology

Volumn 214, Issue 1-2, 2006, Pages 103-113

  Lin, Bor Ruei ^a   Gierasch, Lila M ^b   Jiang, Chun ^a   Tai, Phang C ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

E. coli inverted membrane vesicle; Protein conducting channel; SecA; Signal peptide; Voltage clamp; Xenopus oocyte

Indexed keywords

ADENOSINE TRIPHOSPHATE; BETA,GAMMA METHYLENEADENOSINE TRIPHOSPHATE; LAMB SIGNAL PEPTIDE; OUTER MEMBRANE PROTEIN A; POTASSIUM CHLORIDE; PROTEIN PRECURSOR; PROTEIN SECA; PUROMYCIN; SIGNAL PEPTIDE; SODIUM AZIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL MEMBRANE; CONTROLLED STUDY; ELECTRODE; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; HYDROLYSIS; ION CURRENT; MEMBRANE VESICLE; NONHUMAN; OOCYTE; VOLTAGE CLAMP; XENOPUS;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CELL MEMBRANE; ENZYME INHIBITORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FEMALE; ION CHANNELS; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; OOCYTES; POTASSIUM CHLORIDE; PROTEIN PRECURSORS; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; SODIUM AZIDE; XENOPUS LAEVIS;

ESCHERICHIA COLI;

EID: 34447119691     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-006-0079-1     Document Type: Article

References (49)

1. Breyton C., Haase W., Rapoport T.A., Kuhlbrandt W., Collinson I. 2002. Three-dimensional structure of the bacterial protein-translocation complex SecYEG. Nature 418:662-665
2. Cabelli R.J., Chen L., Tai P.C., Oliver D.B. 1988. SecA protein is required for secretory protein translocation into E. coli membrane vesicles. Cell 55:683-692
3. Cabelli R.J., Dolan K.M., Qian L.P., Oliver D.B. 1991. Characterization of membrane-associated and soluble states of SecA protein from wild-type and SecA51(TS) mutant strains of Escherichia coli. J. Biol. Chem. 266:24420-24427
4. Chen L., Tai P.C. 1986a. Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles. J. Bacteriol. 168:828-832
5. Chen L., Tai P.C., Briggs M.S., Gierasch L.M. 1987. Protein translocation into Escherichia coli membrane vesicles is inhibited by functional synthetic signal peptides. J. Biol. Chem. 262:1427-1429
6. Chen L.L., Tai P.C. 1985. ATP is essential for protein translocation into Escherichia coli membrane vesicles. Proc. Natl. Acad. Sci. USA 82:4384-4388
7. +-ATPase and proton motive force in ATP-dependent protein translocation in vitro. J. Bacteriol. 167:389-392
8. Chen X., Brown T., Tai P.C. 1998. Identification and characterization of protease-resistant SecA fragments: SecA has two membrane-integral forms. J. Bacteriol. 180:527-537
9. Chen X., Xu H., Tai P.C. 1996. A significant fraction of functional SecA is permanently embedded in the membrane. SecA cycling on and off the membrane is not essential during protein translocation. J. Biol. Chem. 271:29698-29706
10. Darzacq X., Singer R.H., Shav-Tal Y. 2005. Dynamics of transcription and mRNA export. Curr. Opin. Cell Biol. 17:332-339
11. Economou A. 1999. Following the leader: Bacterial protein export through the Sec pathway. Trends Microbiol. 7:315-320
12. Economou A., Wickner W. 1994. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78:835-843
13. Emr S.D., Silhavy T.J. 1983. Importance of secondary structure in the signal sequence for protein secretion. Proc. Natl. Acad. Sci. USA 80:4599-4603
14. Geller B.L., Movva N.R., Wickner W. 1986. Both ATP and the electrochemical potential are required for optimal assembly of proOmpA into Escherichia coli inner membrane vesicles. Proc. Natl. Acad. Sci. USA 83:4219-4222
15. Gorlich D., Prehn S., Hartmann E., Kalies K.U., Rapoport T.A. 1992. A mammalian homolog of Sec61p and SecYp is associated with ribosomes and nascent polypeptides during translocation. Cell 71:489-503
16. Hanein D., Matlack K.E., Jungnickel B., Plath K., Kalies K.U., Miller K.R., Rapoport T.A., Akey C.W. 1996. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell 87:721-732
17. Jungnickel B., Rapoport T.A., Hartmann E. 1994. Protein translocation: Common themes from bacteria to man. FEBS Lett. 346:73-77
18. Kawasaki S., Mizushima S., Tokuda H. 1993. Membrane vesicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and countermovement of protons in a SecA- and presecretory protein-dependent manner. J. Biol. Chem. 268:8193-8198
19. Koren R., Burstein Y., Soreq H. 1983. Synthetic leader peptide modulates secretion of proteins from microinjected Xenopus oocytes. Proc. Natl. Acad. Sci. USA 80:7205-7209
20. Manting E.H., Driessen A.J. 2000. Escherichia coli translocase: The unravelling of a molecular machine. Mol. Microbiol. 37:226-238
21. Manting E.H., van Der Does C., Remigy H., Engel A., Driessen A.J. 2000. SecYEG assembles into a tetramer to form the active protein translocation channel. EMBO J. 19:852-861
22. + channels by protein kinase C. Proc. Natl. Acad. Sci. USA 101:1087-1092
23. Meyer T.H., Menetret J.F., Breitling R., Miller K.R., Akey C.W., Rapoport T.A. 1999. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J. Mol. Biol. 285:1789-1800
24. Mitra K., Schaffitzel C., Shaikh T., Tama F., Jenni S., Brooks C.L., 3rd, Ban N., Frank J. 2005. Structure of the E. coli protein-conducting channel bound to a translating ribosome. Nature 438:318-324
25. Mizushima S., Tokuda H. 1990. In vitro translocation of bacterial secretory proteins and energy requirements. J. Bioenerg. Biomembr. 22:389-399
26. Mori H., Ito K. 2001. The Sec protein-translocation pathway. Trends Microbiol. 9:494-500
27. Mowry K.L., Cote C.A. 1999. RNA sorting in Xenopus oocytes and embryos. FASEB J. 13:435-445
28. Oliver D.B., Beckwith J. 1982. Regulation of a membrane component required for protein secretion in Escherichia coli. Cell 30:311-319
29. Palacios I.M., St Johnston D. 2001. Getting the message across: The intracellular localization of mRNAs in higher eukaryotes. Annu. Rev. Cell Dev. Biol. 17:569-614
30. Schiebel E., Wickner W. 1992. Preprotein translocation creates a halide anion permeability in the Escherichia coli plasma membrane. J. Biol. Chem. 267:7505-7510
31. Schmidt M.G., Rollo E.E., Grodberg J., Oliver D.B. 1988. Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli. J. Bacteriol. 170:3404-3414
32. Simon S.M., Blobel G. 1991. A protein-conducting channel in the endoplasmic reticulum. Cell 65:371-380
33. Simon S.M., Blobel G. 1992. Signal peptides open protein-conducting channels in E. coli. Cell 69:677-684
34. Simon S.M., Blobel G., Zimmerberg J. 1989. Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli. Proc. Natl. Acad. Sci. USA 86:6176-6180
35. Soreq H., Seidman S. 1992. Xenopus oocyte microinjection: From gene to protein. Methods Enzymol. 207:225-265
36. Tai P.C., Tian G., Xu H., Lian J.P., Yu J.N. 1991. In vitro protein translocation into Escherichia coli inverted membrane vesicles. Methods Cell Biol. 34:167-187
37. Uchida K., Mori H., Mizushima S. 1995. Stepwise movement of preproteins in the process of translocation across the cytoplasmic membrane of Escherichia coli. J. Biol. Chem. 270:30862-30868
38. van der Wolk J.P., de Wit J.G., Driessen A.J. 1997. The catalytic cycle of the Escherichia coli SecA ATPase comprises two distinct preprotein translocation events. EMBO J. 16:7297-7304
39. Veenendaal A.K., van der Does C., Driessen A.J. 2004. The protein-conducting channel SecYEG. Biochim. Biophys. Acta 1694:81-95
40. Vrontou E., Economou A. 2004. Structure and function of SecA, the preprotein translocase nanomotor. Biochim. Biophys. Acta 1694:67-80
41. Wang H.W., Chen Y., Yang H., Chen X., Duan M.X., Tai P.C., Sui S.F. 2003. Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels. Proc. Natl. Acad. Sci. USA 100:4221-4226
42. Watanabe M., Blobel G. 1993. SecA protein is required for translocation of a model precursor protein into inverted vesicles of Escherichia coli plasma membrane. Proc. Natl. Acad. Sci. USA 90:9011-9015
43. Watanabe M., Nicchitta C.V., Blobel G. 1990. Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins. Proc. Natl. Acad. Sci. USA 87:1960-1964
44. Wickner W., Leonard M.R. 1996. Escherichia coli preprotein translocase. J. Biol. Chem. 271:29514-29516
45. Wirth A., Jung M., Bies C., Frien M., Tyedmers J., Zimmermann R., Wagner R. 2003. The Sec61p complex is a dynamic precursor activated channel. Mol. Cell 12:261-268
46. ATP channels by intracellular acidosis. J. Biol. Chem. 276:12898-12902
47. Yang Y.B., Lian J., Tai P.C. 1997a. Differential translocation of protein precursors across SecY-deficient membranes of Escherichia coli: SecY is not obligatorily required for translocation of certain secretory proteins in vitro. J. Bacteriol. 179:7386-7393
48. Yang Y.B., Yu N., Tai P.C. 1997b. SecE-depleted membranes of Escherichia coli are active. SecE is not obligatorily required for the in vitro translocation of certain protein precursors. J. Biol. Chem. 272:13660-13665
49. Zhu G., Qu Z., Cui N., Jiang C. 1999. Suppression of Kir2.3 activity by protein kinase C phosphorylation of the channel protein at threonine 53. J. Biol. Chem. 274:11643-11646