The Sec61p complex is a dynamic precursor activated channel

Molecular Cell

Volumn 12, Issue 1, 2003, Pages 261-268

  Wirth, Andreas ^a   Jung, Martin ^b   Bies, Christiane ^b   Frien, Michael ^b   Tyedmers, Jens ^b   Zimmermann, Richard ^b   Wagner, Richard ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ION CHANNEL; POLYPEPTIDE; PROTEIN; PROTEIN SEC61; UNCLASSIFIED DRUG;

ARTICLE; CHANNEL GATING; ENDOPLASMIC RETICULUM; ION CONDUCTANCE; LIPID BILAYER; NONHUMAN; PRECURSOR; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; TRANSPORT KINETICS;

EID: 0042671220     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(03)00283-1     Document Type: Article

References (23)

1. Akabas M.H., Cohen F.S., Finkelstein A. Separation of the osmotically driven fusion event from vesicle-planar membrane attachment in a model system for exocytosis. J. Cell Biol. 98:1984;1063-1071.
2. Beckmann R., Bubeck D., Grassucci R., Penczek P., Verschoor A., Blobel G., Frank J. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex. Science. 278:1997;2123-2126.
3. Beckmann R., Spahn C.M., Eswar N., Helmers J., Penczek P.A., Sali A., Frank J., Blobel G. Architecture of the protein-conducting channel associated with the translating 80S ribosome. Cell. 107:2001;361-372.
4. Blobel G., Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67:1975;835-851.
5. Dierks T., Volkmer J., Schlenstedt G., Jung C., Sandholzer U., Zachmann K., Schlotterhose P., Neifer K., Schmidt B., Zimmermann R. A microsomal ATP-binding protein involved in efficient protein transport into the mammalian endoplasmic reticulum. EMBO J. 15:1996;6931-6942.
6. Gilmore R., Blobel G. Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants. Cell. 42:1985;497-505.
7. Görlich D., Rapoport T.A. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. Cell. 75:1993;615-630.
8. Haigh N.G., Johnson A.E. A new role for BiP. closing the aqueous translocon pore during protein integration into the ER membrane J. Cell Biol. 156:2002;261-270.
9. Hamman B.D., Chen J.C., Johnson E.E., Johnson A.E. The aqueous pore through the translocon has a diameter of 40-60 A during co-translational protein translocation at the ER membrane. Cell. 89:1997;535-544.
10. Hamman B.D., Hendershot L.M., Johnson A.E. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocon pore before and early in translocation. Cell. 92:1998;747-758.
11. Hanein D., Matlack K.E.S., Jungnickel B., Plath K., Kalies K.-U., Miller K.R., Rapoport T.A., Akey C.W. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell. 87:1996;721-732.
12. Hille B. Ionic Channels of Excitable Membranes. Third Edition:2001;Sinauer Ass. Inc, Sunderland, MA.
13. Hinnah S.C., Wagner R., Sveshnikova N., Harrer R., Soll J. The chloroplast protein import channel Toc75. pore properties and interaction with transit peptides Biophys. J. 83:2002;899-911.
14. Klappa P., Mayinger P., Pipkorn R., Zimmermann M., Zimmermann R. A microsomal protein is involved in ATP-dependent transport of presecretory proteins into mammalian microsomes. EMBO J. 10:1991;2795-2803.
15. Koopmann J.O., Albring J., Huter E., Bulbuc N., Spee P., Neefjes J., Hammerling G.J., Momburg F. Export of antigenic peptides from the endoplasmic reticulum intersects with retrograde protein translocation through the Sec61p channel. Immunity. 13:2000;117-127.
16. Lauring B., Sakai H., Kreibich G., Wiedmann M. Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA. 92:1995;5411-5415.
17. Liao S., Lin J., Do H., Johnson A.E. Both lumenal and cytosolic gating of the aqueous ER translocon pore are regulated from inside the ribosome during membrane protein integration. Cell. 90:1997;31-41.
18. Menetret J.F., Neuhof A., Morgan D.G., Plath K., Radermacher M., Rapoport T.A., Akey C.W. The structure of ribosome-channel complexes engaged in protein translocation. Mol. Cell. 6:2000;1219-1232.
19. Nicchitta C.V., Blobel G. Lumenal proteins of the mammalian endoplasmic reticulum are required to complete protein translocation. Cell. 73:1993;989-998.
20. Simon S.M., Blobel G. A protein-conducting channel in the endoplasmic reticulum. Cell. 65:1991;371-380.
21. Simon S.M., Blobel G., Zimmerberg J. Large aqueous channels in membrane vesicles derived from the rough endoplasmic reticulum of canine pancreas or the plasma membrane of Escherichia coli. Proc. Natl. Acad. Sci. USA. 86:1989;6176-6180.
22. Tyedmers J., Lerner M., Wiedmann M., Volkmer J., Zimmermann R. Polypeptide-binding proteins mediate completion of co-translational protein translocation into the mammalian endoplasmic reticulum. EMBO Rep. 4:2003;505-510.
23. Woodbury D.J., Hall J.E. Role of channels in the fusion of vesicles with a planar bilayer. Biophys. J. 54:1988;1053-1063.