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Around 575 nm, the signals for glucose oxidase and flavodoxin are dominated by ground-state bleaching, as indicated by the polarization ratio of 3, as expected for pumping and probing the same transition. For photolyase, we assume the signal in this region to be a superposition of ground-state bleaching of the flavin (ΔB) and induced absorption (ΔA). For the ground-state bleaching ΔB∥/ΔB_ = 3. From the decomposition of Figure 6a, we can estimate the ratio a of the contributions of Isotropic ΔA and ΔB, α = (ΔA∥ + 2ΔA⊥)/(ΔB∥ + 2ΔB⊥). From the polarization data of Figure 5a we can estimate the polarization ratio of the total signal: β = (ΔA∥ + Δβ∥)/(ΔA⊥ + ΔB⊥.). Solving this set of equations yields the polarization ratio ΔA∥/ΔA⊥ = (β + 2χ)/(1 - χ), with χ = (β - 3)/5α. For α ∼ -0.4 and β ∼ 8, we find a value of ∼ 0.9.
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2 > 1/(0.7 ps).
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3442899717
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Electron donors could be aromatic residues. For photolyase it could also be the adenine moiety of the cofactor.
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Transition moment dipole directions for adenine radicals have not been reported, but supposedly lie in the adenine plane.
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