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Volumn 108, Issue 28, 2004, Pages 10160-10167

Excited-state properties of flavin radicals in flavoproteins: Femtosecond spectroscopy of DNA photolyase, glucose oxidase, and flavodoxin

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION; AMINO ACIDS; ANISOTROPY; BLEACHING; CATALYST DEACTIVATION; CHARGE TRANSFER; DENSITY (SPECIFIC GRAVITY); GLUCOSE; INFRARED RADIATION; MOLECULAR STRUCTURE; PROTEINS; QUENCHING; REDOX REACTIONS; SPECTROSCOPY; ULTRAVIOLET SPECTROSCOPY;

EID: 3442876665     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp037837b     Document Type: Article
Times cited : (39)

References (48)
  • 4
    • 0038305458 scopus 로고    scopus 로고
    • Sancar, A. Chem. Rev. 2003, 103, 2203-2238.
    • (2003) Chem. Rev. , vol.103 , pp. 2203-2238
    • Sancar, A.1
  • 18
    • 0030952249 scopus 로고    scopus 로고
    • Monaco, H. L. EMBO J. 1997, 16, 1475-1483.
    • (1997) EMBO J. , vol.16 , pp. 1475-1483
    • Monaco, H.L.1
  • 25
    • 3442886888 scopus 로고    scopus 로고
    • note
    • The yield and stability of the FMNḢ were tested for flavodoxin from Synechococcus sp. PCC 7002 and D. gigas. The latter was found to be more favorable.
  • 34
    • 3442902006 scopus 로고    scopus 로고
    • note
    • Around 575 nm, the signals for glucose oxidase and flavodoxin are dominated by ground-state bleaching, as indicated by the polarization ratio of 3, as expected for pumping and probing the same transition. For photolyase, we assume the signal in this region to be a superposition of ground-state bleaching of the flavin (ΔB) and induced absorption (ΔA). For the ground-state bleaching ΔB∥/ΔB_ = 3. From the decomposition of Figure 6a, we can estimate the ratio a of the contributions of Isotropic ΔA and ΔB, α = (ΔA∥ + 2ΔA⊥)/(ΔB∥ + 2ΔB⊥). From the polarization data of Figure 5a we can estimate the polarization ratio of the total signal: β = (ΔA∥ + Δβ∥)/(ΔA⊥ + ΔB⊥.). Solving this set of equations yields the polarization ratio ΔA∥/ΔA⊥ = (β + 2χ)/(1 - χ), with χ = (β - 3)/5α. For α ∼ -0.4 and β ∼ 8, we find a value of ∼ 0.9.
  • 37
    • 3442897991 scopus 로고    scopus 로고
    • Personal communication
    • Moura, J. J. G. Personal communication.
    • Moura, J.J.G.1
  • 41
    • 3442887146 scopus 로고    scopus 로고
    • note
    • 2 > 1/(0.7 ps).
  • 42
    • 3442899717 scopus 로고    scopus 로고
    • note
    • Electron donors could be aromatic residues. For photolyase it could also be the adenine moiety of the cofactor.
  • 46
    • 3442887145 scopus 로고    scopus 로고
    • note
    • Transition moment dipole directions for adenine radicals have not been reported, but supposedly lie in the adenine plane.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.