Identification of Cys385 in the isolated kinase insertion domain of heme-regulated eIF2α kinase (HRI) as the heme axial ligand by site-directed mutagenesis and spectral characterization

Journal of Inorganic Biochemistry

Volumn 101, Issue 8, 2007, Pages 1172-1179

  Hirai, Kyoko ^a   Martinkova, Marketa ^a   Igarashi, Jotaro ^a   Saiful, Islam ^a   Yamauchi, Seigo ^a   El Mashtoly, Samir ^b   Kitagawa, Teizo ^b   Shimizu, Toru ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b NATIONAL INSTITUTES OF NATURAL SCIENCES   (Japan)

Author keywords

ESR; Heme axial ligands; Kinase; Resonance Raman; Translation

Indexed keywords

CYSTEINE; FERRIC ION; FERROUS ION; HEME; HEME REGULATED EIF 2ALPHA KINASE; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ISOLATION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SITE DIRECTED MUTAGENESIS;

ANIMALS; CYSTEINE; EIF-2 KINASE; ELECTRON SPIN RESONANCE SPECTROSCOPY; GENE EXPRESSION REGULATION; HEME; LIGANDS; MICE; MUTAGENESIS, INSERTIONAL; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SERINE; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTRUM ANALYSIS, RAMAN;

EID: 34347382331     PISSN: 01620134     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jinorgbio.2007.05.004     Document Type: Article

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