Allosteric mechanisms in cytochrome P450 3A4 studied by high-pressure spectroscopy: Pivotal role of substrate-induced changes in the accessibility and degree of hydration of the heme pocket

Biochemistry

Volumn 46, Issue 26, 2007, Pages 7852-7864

  Davydov, Dmitri R ^a   Baas, Bradley J ^b   Sligar, Stephen G ^b   Halpert, James R ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BROMOCRIPTINE (BCT); CYTOCHROME P450 3A4; HIGH-PRESSURE SPECTROSCOPY; NANODISCS;

HORMONES; HYDROSTATIC PRESSURE; IRON; MONOMERS; OLIGOMERS; PARAMETER ESTIMATION;

ENZYMES;

1 PYRENEBUTANOL; BROMOCRIPTINE; CYTOCHROME P450 3A4; HEME; OLIGOMER; PYRENE DERIVATIVE; TESTOSTERONE; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; CHEMICAL REACTION; ENZYME SUBSTRATE; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; HIGH PRESSURE SPECTROSCOPY; HYDRATION; HYDROSTATIC PRESSURE; NONHUMAN; PRINCIPAL COMPONENT ANALYSIS; PRIORITY JOURNAL; SPECTROSCOPY;

ALLOSTERIC REGULATION; BROMOCRIPTINE; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON SPIN RESONANCE SPECTROSCOPY; HEME; HYDROSTATIC PRESSURE; NANOTECHNOLOGY; PRINCIPAL COMPONENT ANALYSIS; PROTEIN CONFORMATION; PYRENES; SPECTROPHOTOMETRY, ULTRAVIOLET; TESTOSTERONE;

EID: 34347326224     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi602400y     Document Type: Article

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