Substrate activity screening (SAS): A general procedure for the preparation and screening of a fragment-based non-peptidic protease substrate library for inhibitor discovery

Nature Protocols

Volumn 2, Issue 2, 2007, Pages 424-433

  Patterson, Andrew W ^a   Wood, Warren J L ^a,b   Ellman, Jonathan A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOCOUMARIN DERIVATIVE; CYSTEINE PROTEINASE; PROTEINASE INHIBITOR; SERINE PROTEINASE; SUICIDE SUBSTRATE; 7 AMINO 4 METHYLCOUMARIN 3 ACETIC ACID; 7-AMINO-4-METHYLCOUMARIN-3-ACETIC ACID; 9 FLUORENYLMETHOXYCARBONYL; 9-FLUORENYLMETHOXYCARBONYL; COUMARIN DERIVATIVE; FLUORENE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CHEMICAL STRUCTURE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; PHARMACOPHORE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SCREENING TEST; SOLID PHASE SYNTHESIS; CHEMISTRY; COMBINATORIAL CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; SPECTROFLUOROMETRY; STRUCTURE ACTIVITY RELATION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMBINATORIAL CHEMISTRY TECHNIQUES; COUMARINS; FLUORENES; MOLECULAR STRUCTURE; PROTEASE INHIBITORS; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 34347226326     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.28     Document Type: Article

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