Molecular cloning and characterization of thermostable β-lactam acylase with broad substrate specificity from Bacillus badius

Journal of Bioscience and Bioengineering

Volumn 103, Issue 5, 2007, Pages 457-463

  Rajendhran, Jeyaprakash ^a   Gunasekaran, Paramasamy ^a  

^a Center for Excellence in Genomic Sciences   (India)

Author keywords

gene expression; genome walking polymerase chain reaction; His tagged protein; penicillin acylase; promoter; recombinant enzyme

Indexed keywords

DIRECT GENOME SEQUENCING; PENICILLIN ACYLASE; POLYMERASE CHAIN REACTION; THERMOSTABILITY;

AMINO ACIDS; CLONING; MOLECULAR ORIENTATION; SYNTHESIS (CHEMICAL);

ENZYMES;

6 NITRO 3 PHENYACETYLAMIDOBENZOIC ACID; AMIDASE; AMINO ACID; BENZOIC ACID DERIVATIVE; BETA LACTAM ACYLASE; BETA LACTAM DERIVATIVE; CHROMOGENIC SUBSTRATE; NITRILOTRIACETIC ACID; PENICILLIN G; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BETA CHAIN; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE SEQUENCE; HYDROLYSIS; MOLECULAR CLONING; OPEN READING FRAME; PAC GENE; PH; POLYMERASE CHAIN REACTION; PROMOTER REGION; THERMOSTABILITY;

BACILLUS; CLONING, MOLECULAR; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; LACTAMS; PENICILLIN AMIDASE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

BACILLUS BADIUS; ESCHERICHIA COLI;

EID: 34250878415     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.103.457     Document Type: Article

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