Construction of a thermostable cytochrome P450 chimera derived from self-sufficient mesophilic parents

Applied Microbiology and Biotechnology

Volumn 75, Issue 5, 2007, Pages 1055-1061

  Eiben, Sabine ^a   Bartelmäs, Heike ^a   Urlacher, Vlada B ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Chimera; Cytochrome P450 monooxygenase; Self sufficient; Thermostability

Indexed keywords

CHIMERA; CYTOCHROME P450 MONOOXYGENASE; HYDROXYLATE SUBSTRATES; PARENTAL ENZYMES;

BACTERIA; BIOCATALYSTS; HYDROXYLATION; THERMODYNAMIC STABILITY;

PROTEINS;

BACTERIAL ENZYME; BACTERIAL PROTEIN; CHIMERIC PROTEIN; CYTOCHROME P450; CYTOCHROME P450 BM3; CYTOCHROME P450 CYP102 A3; HYBRID PROTEIN; OXIDOREDUCTASE; UNCLASSIFIED DRUG;

BACTERIUM; CATALYSIS; ENZYME ACTIVITY; PROTEIN;

ARTICLE; BACILLUS MEGATERIUM; BACILLUS SUBTILIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; HIGH TEMPERATURE; HYDROXYLATION; MESOPHILIC BACTERIUM; NONHUMAN; PROTEIN DOMAIN; SPECTROPHOTOMETRY; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME STABILITY; HEAT; MIXED FUNCTION OXYGENASES; RECOMBINANT FUSION PROTEINS;

BACILLUS MEGATERIUM; BACILLUS SUBTILIS;

EID: 34250679406     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0922-z     Document Type: Article

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